Q74CB6 (PUR5_GEOSL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase
EC=6.3.3.1

Alternative name(s):
AIR synthase
AIRS
Phosphoribosyl-aminoimidazole synthetase

Gene names

Name:	purM
Ordered Locus Names:	GSU1758

Organism

Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) [Reference proteome] [HAMAP]

Taxonomic identifier

243231 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter › Geobacter sulfurreducens

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP-Rule MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP-Rule MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP-Rule MF_00741		PRO_0000258358

Sequences

	Sequence		Length	Mass (Da)
	Q74CB6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: ACA3088D52A5C6DB	FASTA	348	36,841
	10 20 30 40 50 60 MTKRGFTYKD AGVDIDAGNT FVGLIKPFVK ATSRPEVISD IGGFGGLFSL NTNKYRNPVL 70 80 90 100 110 120 VSGTDGVGTK LKIAMMADRH DTVGIDLVAM CVNDIIVQGA EPLFFLDYFA TGKLDPQRGA 130 140 150 160 170 180 AVVKGISEGC VQAGCALIGG ETAEMPGFYQ PGEYDLAGFT VGVVERDNII DGSSITVGNR 190 200 210 220 230 240 LVGIASSGLH SNGYSLARKI IFESMGLGID SILPGLGMSA ADALLTPTKI YVKTILNLLR 250 260 270 280 290 300 DFHVNGIAHI TGGGLLENVP RVLPNGCKAL VHLDSCPLPP LFSLLQEAGS VERDEMYRTF 310 320 330 340 NCGIGMVLAV PENEADEILI RLSGLQEKAF IIGEIAKCEP GAEMVELV

References

[1]

"Genome of Geobacter sulfurreducens: metal reduction in subsurface environments." Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.C., Heidelberg J.F., Wu D., Wu M., Ward N.L., Beanan M.J., Dodson R.J., Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn M.L., Kolonay J.F. , Sullivan S.A., Haft D.H., Selengut J., Davidsen T.M., Zafar N., White O., Tran B., Romero C., Forberger H.A., Weidman J.F., Khouri H.M., Feldblyum T.V., Utterback T.R., Van Aken S.E., Lovley D.R., Fraser C.M. Science 302:1967-1969(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51573 / DSM 12127 / PCA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017180 Genomic DNA. Translation: AAR35135.1.
RefSeq	NP_952808.1. NC_002939.5.
3D structure databases
HSSP	HSSP built from PDB template 1CLI based on UniProtKB P08178.
ProteinModelPortal	Q74CB6.
SMR	Q74CB6. Positions 6-342.
ModBase	Search...
Protein-protein interaction databases
STRING	243231.GSU1758.
Proteomic databases
PRIDE	Q74CB6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAR35135; AAR35135; GSU1758.
GeneID	2687407.
KEGG	gsu:GSU1758.
PATRIC	22026361. VBIGeoSul17553_1798.
Phylogenomic databases
eggNOG	COG0150.
HOGENOM	HOG000229091.
KO	K01933.
OMA	PRVLPKH.
ProtClustDB	PRK05385.
Enzyme and pathway databases
BioCyc	GSUL243231:GH27-1742-MONOMER.
UniPathway	UPA00074; UER00129.
Family and domain databases
HAMAP	MF_00741_B. AIRS_B.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_GEOSL

Accession

Primary (citable) accession number: Q74CB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program