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Protein

Bifunctional protein HldE

Gene

hldE

Organism
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-glycero-D-manno-heptose 7-phosphate at the C-1 position to selectively form D-glycero-beta-D-manno-heptose-1,7-bisphosphate.UniRule annotation
Catalyzes the ADP transfer from ATP to D-glycero-beta-D-manno-heptose 1-phosphate, yielding ADP-D-glycero-beta-D-manno-heptose.UniRule annotation

Catalytic activityi

D-glycero-beta-D-manno-heptose 7-phosphate + ATP = D-glycero-beta-D-manno-heptose 1,7-bisphosphate + ADP.UniRule annotation
D-glycero-beta-D-manno-heptose 1-phosphate + ATP = ADP-D-glycero-beta-D-manno-heptose + diphosphate.UniRule annotation

Pathwayi: ADP-L-glycero-beta-D-manno-heptose biosynthesis

This protein is involved in step 1 and 3 of the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Bifunctional protein HldE (hldE)
  2. no protein annotated in this organism
  3. Bifunctional protein HldE (hldE)
  4. no protein annotated in this organism
This subpathway is part of the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ADP-L-glycero-beta-D-manno-heptose from D-glycero-beta-D-manno-heptose 7-phosphate, the pathway ADP-L-glycero-beta-D-manno-heptose biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei275 – 2751UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi205 – 2084ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywords - Molecular functioni

Kinase, Nucleotidyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGSUL243231:GH27-2115-MONOMER.
UniPathwayiUPA00356; UER00437.
UPA00356; UER00439.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein HldEUniRule annotation
Including the following 2 domains:
D-beta-D-heptose 7-phosphate kinaseUniRule annotation (EC:2.7.1.167UniRule annotation)
Alternative name(s):
D-beta-D-heptose 7-phosphotransferaseUniRule annotation
D-glycero-beta-D-manno-heptose-7-phosphate kinaseUniRule annotation
D-beta-D-heptose 1-phosphate adenylyltransferaseUniRule annotation (EC:2.7.7.70UniRule annotation)
Alternative name(s):
D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferaseUniRule annotation
Gene namesi
Name:hldEUniRule annotation
Synonyms:rfaE
Ordered Locus Names:GSU2085
OrganismiGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Taxonomic identifieri243231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000000577 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 490490Bifunctional protein HldEPRO_0000080110Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243231.GSU2085.

Structurei

3D structure databases

ProteinModelPortaliQ74BF6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 330330RibokinaseAdd
BLAST
Regioni356 – 490135CytidylyltransferaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the carbohydrate kinase PfkB family.UniRule annotation
In the C-terminal section; belongs to the cytidylyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DW0. Bacteria.
COG2870. LUCA.
HOGENOMiHOG000237584.
InParanoidiQ74BF6.
KOiK03272.
OMAiILHKGHA.
OrthoDBiPOG091H01Y4.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01603. HldE. 1 hit.
InterProiIPR023030. Bifunc_HldE.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR004821. Cyt_trans-like.
IPR011611. PfkB_dom.
IPR011913. RfaE_dom_I.
IPR011914. RfaE_dom_II.
IPR029056. Ribokinase-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
PF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR02198. rfaE_dom_I. 1 hit.
TIGR02199. rfaE_dom_II. 1 hit.
PROSITEiPS00583. PFKB_KINASES_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q74BF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERKNVESLF AHAGAINALV IGDLMLDQYL WGKAERISPE APVQVVDVTR
60 70 80 90 100
EEIRIGGAGN VANNLVALGC RVSVASVVGG DENGTILLHA FSGKGIDVTG
110 120 130 140 150
IVEDPSRTTS RKTRVLASNQ QIVRIDRESR DEIGPDNERR IVDYLNAHGD
160 170 180 190 200
RFNVILVSDY LKGVLTPTLL AEVIAFARKR EIPVVVDPKG SDYAKYRGAT
210 220 230 240 250
VLTPNRKEAE AASGIAIRDD ESLCRAGERL LETADLTALV ITRSEEGMSL
260 270 280 290 300
FLRGGQVVHI PTYAREVFDV TGAGDTVLAV LGMALAGGVG FADGARLANV
310 320 330 340 350
AAGVAVGKVG TSTVSPAEIV GSLGFQHGEG DAKVKNLDVL AGIIAAEKAQ
360 370 380 390 400
GKRVVFTNGC FDLLHVGHVK YLQKARSFGD LLVLGLNSDA SIRRLKGEKR
410 420 430 440 450
PLIGQEERAH ILAALDCIDY VVVFDEDTPL NLIETLRPAV LVKGGDYTLD
460 470 480 490
GVVGREVVES YGGRVELVAF VDGRSTTNII EKILKAYGEE
Length:490
Mass (Da):52,359
Last modified:July 5, 2004 - v1
Checksum:iA03232A3E1584347
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR35461.1.
RefSeqiNP_953134.1. NC_002939.5.
WP_010942727.1. NC_002939.5.

Genome annotation databases

EnsemblBacteriaiAAR35461; AAR35461; GSU2085.
GeneIDi2687883.
KEGGigsu:GSU2085.
PATRICi22027031. VBIGeoSul17553_2121.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR35461.1.
RefSeqiNP_953134.1. NC_002939.5.
WP_010942727.1. NC_002939.5.

3D structure databases

ProteinModelPortaliQ74BF6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243231.GSU2085.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAR35461; AAR35461; GSU2085.
GeneIDi2687883.
KEGGigsu:GSU2085.
PATRICi22027031. VBIGeoSul17553_2121.

Phylogenomic databases

eggNOGiENOG4105DW0. Bacteria.
COG2870. LUCA.
HOGENOMiHOG000237584.
InParanoidiQ74BF6.
KOiK03272.
OMAiILHKGHA.
OrthoDBiPOG091H01Y4.

Enzyme and pathway databases

UniPathwayiUPA00356; UER00437.
UPA00356; UER00439.
BioCyciGSUL243231:GH27-2115-MONOMER.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01603. HldE. 1 hit.
InterProiIPR023030. Bifunc_HldE.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR004821. Cyt_trans-like.
IPR011611. PfkB_dom.
IPR011913. RfaE_dom_I.
IPR011914. RfaE_dom_II.
IPR029056. Ribokinase-like.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01467. CTP_transf_like. 1 hit.
PF00294. PfkB. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
TIGRFAMsiTIGR00125. cyt_tran_rel. 1 hit.
TIGR02198. rfaE_dom_I. 1 hit.
TIGR02199. rfaE_dom_II. 1 hit.
PROSITEiPS00583. PFKB_KINASES_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHLDE_GEOSL
AccessioniPrimary (citable) accession number: Q74BF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: July 5, 2004
Last modified: September 7, 2016
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.