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Q74AZ0 (SYL_GEOSL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:GSU2209
OrganismGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) [Reference proteome] [HAMAP]
Taxonomic identifier243231 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter

Protein attributes

Sequence length824 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 824824Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152020

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif581 – 5855"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5841ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q74AZ0 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 5D5B9DB48DFCED55

FASTA82493,367
        10         20         30         40         50         60 
MQERYIPKNV EGKWQEIWEE NKTFTVTEDP SKPKYYLLEM FPYPSGRIHM GHVRNYSIGD 

        70         80         90        100        110        120 
VVGRFKRMRG FNVLHPMGWD AFGMPAENAA IKHGSHPAKW TYENIDYMRS QLKKMGLSYD 

       130        140        150        160        170        180 
WGRELATCDV DYYKWEQKMF LEMYEKGLVY KKSSFVNWCP ACETVLANEQ VEDGCCWRCD 

       190        200        210        220        230        240 
SDVTQKELDQ WFFRITRYAE ELLEDTWNLP GWPERVLVMQ RNWIGKSFGC EIDFPVEGKV 

       250        260        270        280        290        300 
EKVKVFTTRQ DTLYGATFMS LAPEHPQALE LTTPERRAEV EAFIDKVKKT DKIKRTAEDF 

       310        320        330        340        350        360 
EKEGVFTGAY CINPVTNLRM PVYLANFVLL DYGTGAVMAV PTHDQRDFEF ARTYDLPLQV 

       370        380        390        400        410        420 
VIQPEGETLD PAAMTAAYTE VGTMVNSGPF NGMKSDEAKE KIADYLEQEG VGTKTVNYRL 

       430        440        450        460        470        480 
RDWGISRQRY WGNPIPVINC DICGVVPVPD KDLPVVLPMD AEFTGEGGNP LARVESFVNV 

       490        500        510        520        530        540 
TCPQCGAEAR RETDTMDTFV QSSWYFLRYC CPDFACGPID RARAGYWMPV DQYIGGIEHA 

       550        560        570        580        590        600 
VLHLLYSRFF TKALRDLGYV TVAEPFKNLL TQGMVIKDGA KMSKSKGNVV DPDALIERYG 

       610        620        630        640        650        660 
ADTARLFTLF AAPPEKDLDW SDQGVEGSFR FLNRVWRLVF EVLPFIGSAG KPDPAALGDG 

       670        680        690        700        710        720 
ARDLRRTVHK TIRKVTDDLD ERFHFNTAIS AVMELVNAIQ SFEPKNAPEN APVLREAVES 

       730        740        750        760        770        780 
VVQLLAPFVP HVAEELWESL GHQGGVEASG WPSYDPEATV EEELLIVVQV NGKLRGKVTV 

       790        800        810        820 
AVDAGEEQVK AAAFADDKVK PWLDGKQIRK AIYVPGKLLN IVVG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017180 Genomic DNA. Translation: AAR35585.1.
RefSeqNP_953258.1. NC_002939.5.

3D structure databases

ProteinModelPortalQ74AZ0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243231.GSU2209.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAR35585; AAR35585; GSU2209.
GeneID2687062.
KEGGgsu:GSU2209.
PATRIC22027275. VBIGeoSul17553_2241.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHELEVYT.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycGSUL243231:GH27-2241-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_GEOSL
AccessionPrimary (citable) accession number: Q74AZ0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries