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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei584 – 5841ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciGSUL243231:GH27-2241-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:GSU2209
OrganismiGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Taxonomic identifieri243231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
ProteomesiUP000000577: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 824824Leucine--tRNA ligasePRO_0000152020Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243231.GSU2209.

Structurei

3D structure databases

ProteinModelPortaliQ74AZ0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi581 – 5855"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
InParanoidiQ74AZ0.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q74AZ0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQERYIPKNV EGKWQEIWEE NKTFTVTEDP SKPKYYLLEM FPYPSGRIHM
60 70 80 90 100
GHVRNYSIGD VVGRFKRMRG FNVLHPMGWD AFGMPAENAA IKHGSHPAKW
110 120 130 140 150
TYENIDYMRS QLKKMGLSYD WGRELATCDV DYYKWEQKMF LEMYEKGLVY
160 170 180 190 200
KKSSFVNWCP ACETVLANEQ VEDGCCWRCD SDVTQKELDQ WFFRITRYAE
210 220 230 240 250
ELLEDTWNLP GWPERVLVMQ RNWIGKSFGC EIDFPVEGKV EKVKVFTTRQ
260 270 280 290 300
DTLYGATFMS LAPEHPQALE LTTPERRAEV EAFIDKVKKT DKIKRTAEDF
310 320 330 340 350
EKEGVFTGAY CINPVTNLRM PVYLANFVLL DYGTGAVMAV PTHDQRDFEF
360 370 380 390 400
ARTYDLPLQV VIQPEGETLD PAAMTAAYTE VGTMVNSGPF NGMKSDEAKE
410 420 430 440 450
KIADYLEQEG VGTKTVNYRL RDWGISRQRY WGNPIPVINC DICGVVPVPD
460 470 480 490 500
KDLPVVLPMD AEFTGEGGNP LARVESFVNV TCPQCGAEAR RETDTMDTFV
510 520 530 540 550
QSSWYFLRYC CPDFACGPID RARAGYWMPV DQYIGGIEHA VLHLLYSRFF
560 570 580 590 600
TKALRDLGYV TVAEPFKNLL TQGMVIKDGA KMSKSKGNVV DPDALIERYG
610 620 630 640 650
ADTARLFTLF AAPPEKDLDW SDQGVEGSFR FLNRVWRLVF EVLPFIGSAG
660 670 680 690 700
KPDPAALGDG ARDLRRTVHK TIRKVTDDLD ERFHFNTAIS AVMELVNAIQ
710 720 730 740 750
SFEPKNAPEN APVLREAVES VVQLLAPFVP HVAEELWESL GHQGGVEASG
760 770 780 790 800
WPSYDPEATV EEELLIVVQV NGKLRGKVTV AVDAGEEQVK AAAFADDKVK
810 820
PWLDGKQIRK AIYVPGKLLN IVVG
Length:824
Mass (Da):93,367
Last modified:July 5, 2004 - v1
Checksum:i5D5B9DB48DFCED55
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR35585.1.
RefSeqiNP_953258.1. NC_002939.5.

Genome annotation databases

EnsemblBacteriaiAAR35585; AAR35585; GSU2209.
GeneIDi2687062.
KEGGigsu:GSU2209.
PATRICi22027275. VBIGeoSul17553_2241.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR35585.1.
RefSeqiNP_953258.1. NC_002939.5.

3D structure databases

ProteinModelPortaliQ74AZ0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243231.GSU2209.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAR35585; AAR35585; GSU2209.
GeneIDi2687062.
KEGGigsu:GSU2209.
PATRICi22027275. VBIGeoSul17553_2241.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
InParanoidiQ74AZ0.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciGSUL243231:GH27-2241-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51573 / DSM 12127 / PCA.

Entry informationi

Entry nameiSYL_GEOSL
AccessioniPrimary (citable) accession number: Q74AZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: March 4, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.