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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciGSUL243231:GH27-2580-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid
Ordered Locus Names:GSU2547
OrganismiGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Taxonomic identifieri243231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000000577 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117245Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi243231.GSU2547.

Structurei

3D structure databases

ProteinModelPortaliQ74A44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
InParanoidiQ74A44.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q74A44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTGAVTIIGG GLAGCEAAWQ IAGRGVRVVL REMKPQRYSP AHHLSGLAEL
60 70 80 90 100
VCSNSLRGES LENAVGLLKE ELRRAGSLIM AAADATRVPA GGALAVDREL
110 120 130 140 150
FSSYVTERIE GHPLIELVRE EVTELPAEGV LVIASGPLTS DALAERLKQI
160 170 180 190 200
TGDSLYFYDA IAPIVAADSL DSDKVFRASR YGKGDGDDYL NCPLSEEEYE
210 220 230 240 250
RFVDAVLAAE KVPARDFEKV VHFEGCMPVE EMAERGRETL RFGPLKPVGL
260 270 280 290 300
TDPRTGREPH AVVQLRAENR EGTLFNLVGF QTKLTWPEQR RVFRMIPGLE
310 320 330 340 350
SAEFVRLGSM HRNTFINAPT LLEPTFRLKG DPRTFFAGQI TGVEGYVESA
360 370 380 390 400
GSGFLAGINS ARLVRGEDPA VPPPVTALGA LVAHITTAPA RHFQPMNVNY
410 420 430
GLFPPLEGKV KKKDRRGRLA ERALAELDHW LATV
Length:434
Mass (Da):47,290
Last modified:July 5, 2004 - v1
Checksum:iBCE01F1E79BD63BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR35920.1.
RefSeqiNP_953593.1. NC_002939.5.
WP_010943183.1. NC_002939.5.

Genome annotation databases

EnsemblBacteriaiAAR35920; AAR35920; GSU2547.
GeneIDi2686108.
KEGGigsu:GSU2547.
PATRICi22027945. VBIGeoSul17553_2577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR35920.1.
RefSeqiNP_953593.1. NC_002939.5.
WP_010943183.1. NC_002939.5.

3D structure databases

ProteinModelPortaliQ74A44.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243231.GSU2547.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAR35920; AAR35920; GSU2547.
GeneIDi2686108.
KEGGigsu:GSU2547.
PATRICi22027945. VBIGeoSul17553_2577.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
InParanoidiQ74A44.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiEOG6J74VT.

Enzyme and pathway databases

BioCyciGSUL243231:GH27-2580-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
HAMAPiMF_01037. TrmFO.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR020595. MnmG-rel_CS.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51573 / DSM 12127 / PCA.

Entry informationi

Entry nameiTRMFO_GEOSL
AccessioniPrimary (citable) accession number: Q74A44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: January 20, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.