Q748D6 (MURG_GEOSL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase
EC=2.4.1.227

Alternative name(s):
Undecaprenyl-PP-MurNAc-pentapeptide-UDPGlcNAc GlcNAc transferase

Gene names

Name:	murG
Ordered Locus Names:	GSU3069

Organism

Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA) [Reference proteome] [HAMAP]

Taxonomic identifier

243231 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter › Geobacter sulfurreducens

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II) By similarity. HAMAP-Rule MF_00033
Catalytic activity	UDP-N-acetylglucosamine + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = UDP + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP-Rule MF_00033
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00033
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00033.
Sequence similarities	Belongs to the glycosyltransferase 28 family. MurG subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	UDP-N-acetylgalactosamine biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW lipid glycosylation Inferred from electronic annotation. Source: HAMAP peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol 4-beta-N-acetylglucosaminlytransferase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase HAMAP-Rule MF_00033		PRO_0000225056

Sequences

	Sequence		Length	Mass (Da)
	Q748D6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 2FC5D921AED888A1	FASTA	364	39,639
	10 20 30 40 50 60 MKLLIAGGGT GGHLFPGIAV AEEFLARDKQ NEVLFVGTWK GIEARVLPKT GYRLECITAA 70 80 90 100 110 120 GIRGKGSLAR AKGLAKFLYG YAQSRKILKE FRPDLVLGVG GYASAPTLMA ARGMQIPRFI 130 140 150 160 170 180 HEQNAIPGFT NRMLAKVADK IFISLEESRT YFPEDKTLLT GNPLRRQILE QVALAESRER 190 200 210 220 230 240 GDDAFHLLVF GGSAGAHRIN LTMGEALPSL KEAKGRLRIT HQTGENDLED VTAAYEEQGF 250 260 270 280 290 300 TADVVAFIDS MADAYRWADL IVCRAGATTL AEVTACGKPC IFIPYPHAVD DHQRRNAESL 310 320 330 340 350 360 LKRGAGFVII EQELSGEVLA QAIRDLMDDP ARLKAVGEAA QELARLDAAQ AIVDEMVAST RKEE

References

[1]

"Genome of Geobacter sulfurreducens: metal reduction in subsurface environments." Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.C., Heidelberg J.F., Wu D., Wu M., Ward N.L., Beanan M.J., Dodson R.J., Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn M.L., Kolonay J.F. , Sullivan S.A., Haft D.H., Selengut J., Davidsen T.M., Zafar N., White O., Tran B., Romero C., Forberger H.A., Weidman J.F., Khouri H.M., Feldblyum T.V., Utterback T.R., Van Aken S.E., Lovley D.R., Fraser C.M. Science 302:1967-1969(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51573 / DSM 12127 / PCA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017180 Genomic DNA. Translation: AAR36461.1.
RefSeq	NP_954111.1. NC_002939.5.
3D structure databases
ProteinModelPortal	Q748D6.
ModBase	Search...
Protein-protein interaction databases
STRING	243231.GSU3069.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAR36461; AAR36461; GSU3069.
GeneID	2686309.
KEGG	gsu:GSU3069.
PATRIC	22028989. VBIGeoSul17553_3092.
Phylogenomic databases
eggNOG	COG0707.
HOGENOM	HOG000218323.
KO	K02563.
OMA	DDHQTKN.
ProtClustDB	PRK00726.
Enzyme and pathway databases
BioCyc	GSUL243231:GH27-3018-MONOMER.
UniPathway	UPA00219.
Family and domain databases
HAMAP	MF_00033. MurG.
InterPro	IPR006009. GlcNAc_MurG. IPR004276. Glyco_trans_28. IPR007235. Glyco_trans_28_C. [Graphical view]
PANTHER	PTHR21015:SF22. PTHR21015:SF22. 1 hit.
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF03033. Glyco_transf_28. 1 hit. [Graphical view]
TIGRFAMs	TIGR01133. murG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURG_GEOSL

Accession

Primary (citable) accession number: Q748D6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program