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Q747X9

- SYI_GEOSL

UniProt

Q747X9 - SYI_GEOSL

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Protein
Isoleucine--tRNA ligase
Gene
ileS, GSU3136
Organism
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei560 – 5601Aminoacyl-adenylate By similarity
Binding sitei604 – 6041ATP By similarity
Metal bindingi895 – 8951Zinc By similarity
Metal bindingi898 – 8981Zinc By similarity
Metal bindingi915 – 9151Zinc By similarity
Metal bindingi918 – 9181Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciGSUL243231:GH27-3177-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:GSU3136
OrganismiGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Taxonomic identifieri243231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
ProteomesiUP000000577: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 923923Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098391Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi243231.GSU3136.

Structurei

3D structure databases

ProteinModelPortaliQ747X9.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi601 – 6055"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiYETLYEC.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q747X9-1 [UniParc]FASTAAdd to Basket

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MDYKSTLNLP VTDFPMKANL PQREPEILAA WQQGDLYGTI AKAGKGKPRY    50
VLHDGPPYAN GHIHIGHALN KILKDIILKS KRMQGFDAPY VPGWDCHGLP 100
IELQVEKNLG SKKHETTKLQ MRKQCREYAE KFVHVQRAEF ERLGVLGDWD 150
RPYLTMSYDY EGITARELAR FAENGGLYKG KKPVHWCSSC VTALAEAEVE 200
YADKTSPSIY VKFLLQDDIG GAVPALAGKR VSLVIWTTTP WTIPANLAVA 250
LHPELDYVAL ETGGEVLVVA EGLKDAFMAA TGVQGDVIAT FRADILYRKR 300
CKHPFYDRDS IVLLGEHVTL DAGTGCVHTA PGHGQEDYEL ALVEGLDIYN 350
PVDNRGRYIQ SLEFFGGMFV FDANAAVMEK LREVGALVGQ GSVEHSYPHC 400
WRCKKPIIFR ATEQWFISME KNDLRQKALT EIDRVSWVPK WGRERIHGMI 450
ENRPDWCISR QRSWGVPITA FYCTECGEIL ADGKTMHHVA DLFMAGGADL 500
WYEKEAAELL PPGTVCPQCG KSAFEKEMDI LDVWFDSGVS HAAVLENRPE 550
LGSPANMYLE GSDQHRGWFH SSLLASVGTR GVAPYREVLT HGFVVDGSGR 600
KMSKSVGNVV APEEVIKKYG AEILRLWVAA QDYRDDVRIS QEILTRLAEA 650
YRRIRNTCRY LLGNLNDFDP DVDMVPYDRM TELDRWALHQ LEVLKEKVAA 700
AYNEYEFHIL YHAVNGFCTV EMSAFYLDII KERYTSRKDA PERRSAQSVM 750
YLVLESLVTL MAPVLSFTAD EVWGYMPKRS ESSVHLATFP EFRPEWKDES 800
LVERWARIMA VRGDVSKALE QARVQKTIGH SLDAAVTLAA EPGLLSFLQE 850
YAGELSTIFI VSRVDLVEEG AGDYPAAEGV SGLRIGVSAA PGEKCERCWH 900
YDEEIGGDSE HPTLCPKCAA AVK 923
Length:923
Mass (Da):103,533
Last modified:July 5, 2004 - v1
Checksum:i308E14182570D001
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017180 Genomic DNA. Translation: AAR36527.1.
RefSeqiNP_954177.1. NC_002939.5.
WP_010943758.1. NC_002939.5.

Genome annotation databases

EnsemblBacteriaiAAR36527; AAR36527; GSU3136.
GeneIDi2688441.
KEGGigsu:GSU3136.
PATRICi22029129. VBIGeoSul17553_3161.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017180 Genomic DNA. Translation: AAR36527.1 .
RefSeqi NP_954177.1. NC_002939.5.
WP_010943758.1. NC_002939.5.

3D structure databases

ProteinModelPortali Q747X9.
ModBasei Search...

Protein-protein interaction databases

STRINGi 243231.GSU3136.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAR36527 ; AAR36527 ; GSU3136 .
GeneIDi 2688441.
KEGGi gsu:GSU3136.
PATRICi 22029129. VBIGeoSul17553_3161.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi YETLYEC.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci GSUL243231:GH27-3177-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51573 / DSM 12127 / PCA.

Entry informationi

Entry nameiSYI_GEOSL
AccessioniPrimary (citable) accession number: Q747X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi