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Q747X9

- SYI_GEOSL

UniProt

Q747X9 - SYI_GEOSL

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei560 – 5601Aminoacyl-adenylateUniRule annotation
    Binding sitei604 – 6041ATPUniRule annotation
    Metal bindingi895 – 8951ZincUniRule annotation
    Metal bindingi898 – 8981ZincUniRule annotation
    Metal bindingi915 – 9151ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciGSUL243231:GH27-3177-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:GSU3136
    OrganismiGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
    Taxonomic identifieri243231 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
    ProteomesiUP000000577: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 923923Isoleucine--tRNA ligasePRO_0000098391Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243231.GSU3136.

    Structurei

    3D structure databases

    ProteinModelPortaliQ747X9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi601 – 6055"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiYETLYEC.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q747X9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKSTLNLP VTDFPMKANL PQREPEILAA WQQGDLYGTI AKAGKGKPRY    50
    VLHDGPPYAN GHIHIGHALN KILKDIILKS KRMQGFDAPY VPGWDCHGLP 100
    IELQVEKNLG SKKHETTKLQ MRKQCREYAE KFVHVQRAEF ERLGVLGDWD 150
    RPYLTMSYDY EGITARELAR FAENGGLYKG KKPVHWCSSC VTALAEAEVE 200
    YADKTSPSIY VKFLLQDDIG GAVPALAGKR VSLVIWTTTP WTIPANLAVA 250
    LHPELDYVAL ETGGEVLVVA EGLKDAFMAA TGVQGDVIAT FRADILYRKR 300
    CKHPFYDRDS IVLLGEHVTL DAGTGCVHTA PGHGQEDYEL ALVEGLDIYN 350
    PVDNRGRYIQ SLEFFGGMFV FDANAAVMEK LREVGALVGQ GSVEHSYPHC 400
    WRCKKPIIFR ATEQWFISME KNDLRQKALT EIDRVSWVPK WGRERIHGMI 450
    ENRPDWCISR QRSWGVPITA FYCTECGEIL ADGKTMHHVA DLFMAGGADL 500
    WYEKEAAELL PPGTVCPQCG KSAFEKEMDI LDVWFDSGVS HAAVLENRPE 550
    LGSPANMYLE GSDQHRGWFH SSLLASVGTR GVAPYREVLT HGFVVDGSGR 600
    KMSKSVGNVV APEEVIKKYG AEILRLWVAA QDYRDDVRIS QEILTRLAEA 650
    YRRIRNTCRY LLGNLNDFDP DVDMVPYDRM TELDRWALHQ LEVLKEKVAA 700
    AYNEYEFHIL YHAVNGFCTV EMSAFYLDII KERYTSRKDA PERRSAQSVM 750
    YLVLESLVTL MAPVLSFTAD EVWGYMPKRS ESSVHLATFP EFRPEWKDES 800
    LVERWARIMA VRGDVSKALE QARVQKTIGH SLDAAVTLAA EPGLLSFLQE 850
    YAGELSTIFI VSRVDLVEEG AGDYPAAEGV SGLRIGVSAA PGEKCERCWH 900
    YDEEIGGDSE HPTLCPKCAA AVK 923
    Length:923
    Mass (Da):103,533
    Last modified:July 5, 2004 - v1
    Checksum:i308E14182570D001
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017180 Genomic DNA. Translation: AAR36527.1.
    RefSeqiNP_954177.1. NC_002939.5.
    WP_010943758.1. NC_002939.5.

    Genome annotation databases

    EnsemblBacteriaiAAR36527; AAR36527; GSU3136.
    GeneIDi2688441.
    KEGGigsu:GSU3136.
    PATRICi22029129. VBIGeoSul17553_3161.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017180 Genomic DNA. Translation: AAR36527.1 .
    RefSeqi NP_954177.1. NC_002939.5.
    WP_010943758.1. NC_002939.5.

    3D structure databases

    ProteinModelPortali Q747X9.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243231.GSU3136.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAR36527 ; AAR36527 ; GSU3136 .
    GeneIDi 2688441.
    KEGGi gsu:GSU3136.
    PATRICi 22029129. VBIGeoSul17553_3161.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi YETLYEC.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci GSUL243231:GH27-3177-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51573 / DSM 12127 / PCA.

    Entry informationi

    Entry nameiSYI_GEOSL
    AccessioniPrimary (citable) accession number: Q747X9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3