Q747F5 (HEMH_GEOSL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:	hemH
Ordered Locus Names:	GSU3312

Organism

Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)

Taxonomic identifier

243231 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Ferrochelatase HAMAP MF_00323		PRO_0000175145
Sites
	Metal binding	192	1	Iron By similarity
	Metal binding	271	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q747F5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 67B1EB8A3F981A11	FASTA	317	36,023
	10 20 30 40 50 60 MSDKTAVLLL QMGGPDSLDA VEPFLLNLFS DRDIIRIGPA FLQPFIARLI AKRRSPGVER 70 80 90 100 110 120 KYEEIGGKSP IRELTESQAR ALEDVLGDGY RCFVAMRYWK PSTMEALAAI RREGISRVIA 130 140 150 160 170 180 LSLYPHYSRA TTGSSVNELK RVLSQSGVQF QMMYVDRFFD HPLYIDALAE KIREGLDDFH 190 200 210 220 230 240 PLAEVQVLFS AHSLPQSFID EGDPYLDHIR ETVRLVMERF EGVTHHLAFQ SRAGPVKWLE 250 260 270 280 290 300 PSTDEMLEHL AAHQVKNLLI VPLSFVSDHI ETLHEIDIEY AQEAHKLGYS RFRRSPSLNT 310 SPTFISCLAD LVRRVEG

References

[1]

"Genome of Geobacter sulfurreducens: metal reduction in subsurface environments." Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.C., Heidelberg J.F., Wu D., Wu M., Ward N.L., Beanan M.J., Dodson R.J., Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn M.L., Kolonay J.F. , Sullivan S.A., Haft D.H., Selengut J., Davidsen T.M., Zafar N., White O., Tran B., Romero C., Forberger H.A., Weidman J.F., Khouri H.M., Feldblyum T.V., Utterback T.R., Van Aken S.E., Lovley D.R., Fraser C.M. Science 302:1967-1969(2003) [PubMed: 14671304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51573 / DSM 12127 / PCA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017180 Genomic DNA. Translation: AAR36702.1.
RefSeq	NP_954352.1. NC_002939.4.
3D structure databases
ProteinModelPortal	Q747F5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2686646.
GenomeReviews	Gene locus GSU3312 in contig AE017180_GR.
KEGG	gsu:GSU3312.
NMPDR	fig|243231.1.peg.3290.
PATRIC	22029471. VBIGeoSul17553_3330.
TIGR	GSU3312.
Phylogenomic databases
HOGENOM	HBG697135.
OMA	LLQMGGP.
PhylomeDB	Q747F5.
ProtClustDB	CLSK790160.
Enzyme and pathway databases
BioCyc	GSUL243231:GSU_3312-MONOMER.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_GEOSL

Accession

Primary (citable) accession number: Q747F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program