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Protein

Phosphoenolpyruvate carboxykinase [GTP]

Gene

pckG

Organism
Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.UniRule annotation

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei73SubstrateUniRule annotation1
Metal bindingi221ManganeseUniRule annotation1
Metal bindingi241Manganese; via tele nitrogenUniRule annotation1
Binding sitei263SubstrateUniRule annotation1
Active sitei265UniRule annotation1
Metal bindingi292ManganeseUniRule annotation1
Binding sitei384GTPUniRule annotation1
Binding sitei415GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi264 – 269GTPUniRule annotation6
Nucleotide bindingi508 – 511GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

GTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [GTP]UniRule annotation (EC:4.1.1.32UniRule annotation)
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckGUniRule annotation
Ordered Locus Names:GSU3385
OrganismiGeobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)
Taxonomic identifieri243231 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfuromonadalesGeobacteraceaeGeobacter
Proteomesi
  • UP000000577 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036051 – 618Phosphoenolpyruvate carboxykinase [GTP]Add BLAST618

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243231.GSU3385.

Structurei

3D structure databases

ProteinModelPortaliQ746Y3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 214Substrate bindingUniRule annotation3
Regioni382 – 384Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
InParanoidiQ746Y3.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q746Y3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYELPNNEK LLKWVEEVAI LCEPDRIHWC DGSQEEYDEL CELLVQNGTF
60 70 80 90 100
RKLNPEKRPN SYLAWSDPID VARVEDRTFI CSLSKQDAGP TNNWMNPKEM
110 120 130 140 150
KEKLLGLFKG CMKGRTMYVI PFSMGPLGSP IAKIGVEITD SPYVVVNMRI
160 170 180 190 200
MTRMGKKVLD ILGEQGEFVP CLHSVGAPLE QGQKDSTWPC NKEKYIVHFP
210 220 230 240 250
EERSIWSFGS GYGGNALLGK KCLALRIASK QGKDEGWLAE HMLILGVESP
260 270 280 290 300
TGDKTYVGAA FPSACGKTNF AMLIPPKPLQ DKGWKVTTIG DDIAWIKPGP
310 320 330 340 350
DGQVYAINPE YGYFGVAPGT NVKTNPNAMA SCSANTIFTN VALTPDDDVW
360 370 380 390 400
WEGMTDEPPA QLTDWQGNPW TPDCGRPAAH PNARFTSPAS QCPSIDPDWE
410 420 430 440 450
NPKGVPMSAF IFGGRRKDTV PLVYQAFNWS NGVYLAATLG SETTAAAVGA
460 470 480 490 500
VGNVRRDPFA MLPFCGYHMA DYFGHWLQFG RSIPRPPRIF SVNWFRKDAN
510 520 530 540 550
GKFIWPGYGD NMRVLKWIVE RVQGKAGAVE SPLGWMPRYE DMDWDGLDMP
560 570 580 590 600
REKFNELTSV DRDVWQNELI SHEELFVKLF DRLPKEFLHM RELILSSLWR
610
SPEHWEQIGD VHPEGWNE
Length:618
Mass (Da):69,613
Last modified:July 5, 2004 - v1
Checksum:i2D790FCF9D4692C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR36775.1.
RefSeqiNP_954425.1. NC_002939.5.
WP_010943996.1. NC_002939.5.

Genome annotation databases

EnsemblBacteriaiAAR36775; AAR36775; GSU3385.
GeneIDi2688197.
KEGGigsu:GSU3385.
PATRICi22029625. VBIGeoSul17553_3407.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017180 Genomic DNA. Translation: AAR36775.1.
RefSeqiNP_954425.1. NC_002939.5.
WP_010943996.1. NC_002939.5.

3D structure databases

ProteinModelPortaliQ746Y3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243231.GSU3385.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAR36775; AAR36775; GSU3385.
GeneIDi2688197.
KEGGigsu:GSU3385.
PATRICi22029625. VBIGeoSul17553_3407.

Phylogenomic databases

eggNOGiENOG4105C0M. Bacteria.
COG1274. LUCA.
HOGENOMiHOG000191700.
InParanoidiQ746Y3.
KOiK01596.
OMAiYGENMRV.
OrthoDBiPOG091H0B4F.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00452. PEPCK_GTP. 1 hit.
InterProiIPR018091. PEP_carboxykin_GTP_CS.
IPR013035. PEP_carboxykinase_C.
IPR008209. PEP_carboxykinase_GTP.
IPR008210. PEP_carboxykinase_N.
[Graphical view]
PANTHERiPTHR11561. PTHR11561. 1 hit.
PfamiPF00821. PEPCK. 1 hit.
[Graphical view]
PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
PROSITEiPS00505. PEPCK_GTP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKG_GEOSL
AccessioniPrimary (citable) accession number: Q746Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.