Q746S4 (NUBCD_GEOSL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit B/C/D
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit B/C/D
NDH-1 subunit B/C/D

Gene names

Name:	nuoBCD
Synonyms:	nuoB, nuoC, nuoD
Ordered Locus Names:	GSU3444

Organism

Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA)

Taxonomic identifier

243231 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	792 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01356
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01356
Subunit structure	NDH-1 is composed of about 13 different subunits. Subunits NuoBCD, E, F, and G constitute the peripheral sector of the complex By similarity.
Subcellular location	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP MF_01356.
Sequence similarities	In the N-terminal section; belongs to the complex I 20 kDa subunit family. In the central section; belongs to the complex I 30 kDa subunit family. In the C-terminal section; belongs to the complex I 49 kDa subunit family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 792	792	NADH-quinone oxidoreductase subunit B/C/D HAMAP MF_01356		PRO_0000358644
Regions
	Region	1 – 156	156	NADH dehydrogenase I subunit B By similarity
	Region	230 – 385	156	NADH dehydrogenase I subunit C By similarity
	Region	412 – 792	381	NADH dehydrogenase I subunit D By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q746S4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 8BE2808B4070E130	FASTA	792	90,137
	10 20 30 40 50 60 MSETEVPQNN IILASLDDLI NWGRANSLWP MFFGLSCCFV EMMTSFTSRY DVSRFGAEVL 70 80 90 100 110 120 RGTPREADLM VIAGTVFKKM APSILRLYEQ MAEPKWVISM GSCANSGGMY DVYSVVQGVN 130 140 150 160 170 180 QIIPVDVHVP GCPPRPEAFL QGLMLLQEKI RREERPARKV LHLAGGSEGT TRPVLVDGVT 190 200 210 220 230 240 KSRDTRGPGM EGIAIRGTSV QHPHFPMPRS DEMWRPPAPK HQFPDFGLAG ELETAFGHRV 250 260 270 280 290 300 VRDDHATDML TYRCPPELLP DVLRHLKTRS AAPFRRLEDV ACVDESCRRE RSRFPDFTVN 310 320 330 340 350 360 YHLLNFHIPG HLRIKAELRG DTPEIPSATS VFPAADWYER EAFDMYGIQF AGHPNLRRIL 370 380 390 400 410 420 MPPDWEGHPL RKNHPFRATE MHPYTTDDAR RHQALPASDF FDRIDEETLI LNLGPQHPGT 430 440 450 460 470 480 HGIIRFVLKL DGEEIVDMDT DIGYHHRGAE KIGERQHWNQ FIPYTDRIDY LAGVQNNLAY 490 500 510 520 530 540 VNSVERLCGI TVPDRGIAIR VMLAELFRIA NHLVWLGTFA ADVGAMTPVF YTFTDREKIF 550 560 570 580 590 600 DIVEMVTGGR MHPSWFRIGG VADDLPEGWD GAVKAFLDWM PGRLKEYEDL LKGNPIFRER 610 620 630 640 650 660 LKGVGVITGD EALEWGITGP NLRACGVEWD LRKKIPYNGY QHFHFEVPTE EGGDCWARYR 670 680 690 700 710 720 VRIEEIRQSL HIVRQCWKEM PAGRWITDDY RYVLPKKRDA LHDIESLIHH FINATRGMAP 730 740 750 760 770 780 PKGENYSAIE APKGENGYFV VSDGLNVPYR VRIKTPSFPH IQALPLMSRG WLVADFLAII 790 GSIDFVLADL DR

References

[1]

"Genome of Geobacter sulfurreducens: metal reduction in subsurface environments." Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.C., Heidelberg J.F., Wu D., Wu M., Ward N.L., Beanan M.J., Dodson R.J., Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn M.L., Kolonay J.F. , Sullivan S.A., Haft D.H., Selengut J., Davidsen T.M., Zafar N., White O., Tran B., Romero C., Forberger H.A., Weidman J.F., Khouri H.M., Feldblyum T.V., Utterback T.R., Van Aken S.E., Lovley D.R., Fraser C.M. Science 302:1967-1969(2003) [PubMed: 14671304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51573 / DSM 12127 / PCA.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017180 Genomic DNA. Translation: AAR36834.1.
RefSeq	NP_954484.1. NC_002939.4.
3D structure databases
HSSP	HSSP built from PDB template 2FUG based on UniProtKB Q56218.
ProteinModelPortal	Q746S4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2688148.
GenomeReviews	Gene locus GSU3444 in contig AE017180_GR.
KEGG	gsu:GSU3444.
NMPDR	fig|243231.1.peg.3422.
PATRIC	22029745. VBIGeoSul17553_3466.
TIGR	GSU3444.
Phylogenomic databases
HOGENOM	HBG459705.
OMA	PHLQQIP.
PhylomeDB	Q746S4.
ProtClustDB	PRK13292.
Enzyme and pathway databases
BioCyc	GSUL243231:GSU_3444-MONOMER.
Family and domain databases
HAMAP	MF_01356. NDH1_NuoB. Fused. [Tree] MF_01359. NDH1_NuoCD_1. Fused. [Tree]
InterPro	IPR010219. NADH_DH_1_suD. IPR010218. NADH_DH_suC. IPR001135. NADH_Q_OxRdtase_suD. IPR006137. NADH_UbQ_OxRdtase-like_20kDa. IPR001268. NADH_UbQ_OxRdtase_30kDa_su. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR020396. NADH_UbQ_OxRdtase_CS. IPR006138. NADH_UQ_OxRdtase_20Kd_su. IPR022885. NDH1_su_D/H. [Graphical view]
Gene3D	G3DSA:3.40.50.700. G3DSA:3.40.50.700. 1 hit.
KO	K13380.
Pfam	PF00329. Complex1_30kDa. 1 hit. PF00346. Complex1_49kDa. 1 hit. PF01058. Oxidored_q6. 1 hit. [Graphical view]
ProDom	PD001581. NADH_UbQ_OxRdtase_30kDa_su. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01957. NuoB_fam. 1 hit. TIGR01962. NuoD. 1 hit.
PROSITE	PS01150. COMPLEX1_20K. False negative. PS00542. COMPLEX1_30K. 1 hit. PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUBCD_GEOSL

Accession

Primary (citable) accession number: Q746S4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program