Reviewed, UniProtKB/Swiss-Prot Q746S3 (NUOA2_GEOSL)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit A 2
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit A 2
    NDH-1 subunit A 2
    NUO1 2

Gene names

Name:	nuoA2
Ordered Locus Names:	GSU3445

Organism

Geobacter sulfurreducens [Complete proteome] [HAMAP]

Taxonomic identifier

35554 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Deltaproteobacteria › Desulfuromonadales › Geobacteraceae › Geobacter

Protein attributes

Sequence length	142 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol. HAMAP MF_01394
Subunit structure	NDH-1 is composed of 14 different subunits. Subunits nuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the complex I subunit 3 family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP photosynthesis, light reaction Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 142	142	NADH-quinone oxidoreductase subunit A 2 HAMAP MF_01394		PRO_0000362694
Regions
	Transmembrane	18 – 38	21	Potential
	Transmembrane	73 – 93	21	Potential
	Transmembrane	104 – 124	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q746S3-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 70ACF87F9F72B3CE	FASTA	142	15,776
	10 20 30 40 50 60 MQPAGISHSL FPSLPPEFLP LALYTLAASI LIGVLLLAAW WLGAKTTNRN KELPYESGAI 70 80 90 100 110 120 PTGSARLAYP VPFYLIAIFF IVFDVEAAFI FAWATAWREL GLQGLVHITF FIVILLLGLV 130 140 WLWLKGGLDW GPSRARRGHV RD

References

[1]

"Genome of Geobacter sulfurreducens: metal reduction in subsurface environments." Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.C., Heidelberg J.F., Wu D., Wu M., Ward N.L., Beanan M.J., Dodson R.J., Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S., Gwinn M.L., Kolonay J.F. , Sullivan S.A., Haft D.H., Selengut J., Davidsen T.M., Zafar N., White O., Tran B., Romero C., Forberger H.A., Weidman J.F., Khouri H.M., Feldblyum T.V., Utterback T.R., Van Aken S.E., Lovley D.R., Fraser C.M. Science 302:1967-1969(2003) [PubMed: 14671304] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51573 / DSM 12127 / PCA.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017180 Genomic DNA. Translation: AAR36835.1.
RefSeq	NP_954485.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.D.1.5.1. proton-translocating NADH dehydrogenase (NDH) family.
Genome annotation databases
GeneID	2688147.
GenomeReviews	Gene locus GSU3445 in contig AE017180_GR.
KEGG	gsu:GSU3445.
NMPDR	fig|243231.1.peg.3423.
TIGR	GSU3445.
Phylogenomic databases
HOGENOM	Q746S3.
OMA	FIEATIF.
Enzyme and pathway databases
BioCyc	GSUL243231:GSU_3445-MON.
Family and domain databases
HAMAP	MF_01394. [Tree]
InterPro	IPR000440. NADH_UbQ/plastoQ_OxRdtase_su3. [Graphical view]
PANTHER	PTHR11058. Oxidored_q4. 1 hit.
Pfam	PF00507. Oxidored_q4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOA2_GEOSL

Accession

Primary (citable) accession number: Q746S3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)