Q744B6 (Q744B6_MYCPA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL AAS02742.1
Ordered Locus Names:	MAP_0425

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	932 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding RuleBase RU000518 SAAS SAAS005733 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q744B6 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0F9E6367C15D922D	FASTA	932	102,376
	10 20 30 40 50 60 MADPKLKENT SGGNGSRRRL VIVESPTKAR KLASYLGSRY IVESSRGHIR DLPRAAADVP 70 80 90 100 110 120 AKYKSEPWAR LGVNVDADFE PLYIISPEKK HTVSELKGLL KDVDELYLAT DGDREGEAIA 130 140 150 160 170 180 WHLLETLKPN IPVKRMVFHE ITEPAILEAA ENPRDLDIDL VDAQETRRIL DRLYGYEVTP 190 200 210 220 230 240 VLWKKVAPKL SAGRVQSVAT RIIVQRERDR MAFRSASYWD IVAQLDASVS DPQASPPTFA 250 260 270 280 290 300 ARLTSVDGLR VATGRDFDSQ GQLRKADEVI VLDEPQATEL AAGLQGAQLS VASVEEKPYT 310 320 330 340 350 360 RRPYAPFMTS TLQQEAGRKL RFSAERTMSI AQRLYENGYI TYMRTDSTTL SGSAINAART 370 380 390 400 410 420 QARQLYGEEY VSPSPRQYTR KVKNAQEAHE AIRPAGETFA TPDAVRRELD GDEFRLYELI 430 440 450 460 470 480 WQRTVASQMA DARGTTLSLR IGGQAGDRQV VFSASGRTIT FAGFLKAYVE TVDELAGGEA 490 500 510 520 530 540 DDAESRLPQL TQGQRLDAIE LTPDGHATNP PPRYTEASLV KALEELGIGR PSTYSSIIKT 550 560 570 580 590 600 IQDRGYVHKK GSALVPSWVA FAVTGLLEQH FGRLVDYDFT AAMEDELDAI ASGQERRTDW 610 620 630 640 650 660 LNNFYFGGEH GVADSIARSG GLKKLVGVNL EGIDAREVNS IKLFDDEQGR PVYVRVGKNG 670 680 690 700 710 720 PYLERMVTGD DGEPTPQRAN LNDSLTPDEL TLEVAEQLFA TPQEGRVLGV DPATGHEIVA 730 740 750 760 770 780 KDGRYGPYVA EVLPEPPPDD DDGSGAPAKK GKKPTGPKPR TASLLRTMDL QTVTLEDALR 790 800 810 820 830 840 LLSLPRVVGV DPETGEEITA QNGRYGPYLK RGNDSRSLAS EEQLFDITLE EALKIYAEPK 850 860 870 880 890 900 RRGRQGAAAP PLRELGADPA TGKPMVIKDG RFGPYVTDGE TNASLRKGDD VMSLTDQRAA 910 920 930 ELLADRRARG PAKRTAKKTS RKAPAKKAAK RG

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS02742.1.
RefSeq	NP_959359.1. NC_002944.2.
3D structure databases
ProteinModelPortal	Q744B6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000037383; EBMYCP00000035772; EBMYCG00000037378.
GeneID	2718433.
GenomeReviews	Gene locus MAP_0425 in contig AE016958_GR.
KEGG	mpa:MAP0425.
NMPDR	fig|262316.1.peg.425.
PATRIC	17993222. VBIMycAvi108102_0452.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016895.
HOGENOM	HBG512935.
OMA	KKVMPRL.
ProtClustDB	PRK07561.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q744B6_MYCPA

Accession

Primary (citable) accession number: Q744B6

Entry history

Integrated into UniProtKB/TrEMBL:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)