Q743W3 (SYC_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Synonyms:	cysS1
Ordered Locus Names:	MAP_0478

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	468 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 468	468	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000159439
Regions
	Motif	35 – 45	11	"HIGH" region HAMAP MF_00041
	Motif	267 – 271	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	33	1	Zinc By similarity
	Metal binding	211	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Metal binding	240	1	Zinc By similarity
	Binding site	270	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q743W3 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 7BC02C8FE91923E0	FASTA	468	51,929
	10 20 30 40 50 60 MTDRPRLRLH DTAAGAVRDF VPLRDGHVSI YLCGATVQGL PHIGHVRSGV AFDILRRWLI 70 80 90 100 110 120 ALGYDVAFIR NVTDIDDKIL NKAAAAGRPW WEWAATYERA FSAAYDALDV LPPSAEPRAT 130 140 150 160 170 180 GHITQMVELI ERLIEKGHAY TGDGDVYFDV LSYPEYGQLS GHRIDDVHQG EGVASGKRDQ 190 200 210 220 230 240 RDFTLWKGAK PGEPSWPTPW GRGRPGWHTE CVAMAHEYLG PEFDIHCGGM DLVFPHHENE 250 260 270 280 290 300 IAQSRAAGDG FARYWLHNGW VTMGGEKMSK SLGNVLAIPA MLQRVRPAEL RYYLGSAHYR 310 320 330 340 350 360 SMLEFSDTAL QDAVKAYVGV EEFLHRVRVR VGAVEPGEPN PRFADALNDD LAVPAALAEV 370 380 390 400 410 420 HQARAEGNRA LDSGDHEGAL RQARSIRAMM GILGCDPLHE RWETRDETSA ALAAVDVLVR 430 440 450 460 AELQNREKAR EQRNWALADE IRNRLKQAGI EVTDTADGPQ WTLGGDGK

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS02795.1.
RefSeq	NP_959412.1. NC_002944.2.
3D structure databases
ProteinModelPortal	Q743W3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000038943; EBMYCP00000037332; EBMYCG00000038938.
GeneID	2720735.
GenomeReviews	Gene locus MAP_0478 in contig AE016958_GR.
KEGG	mpa:MAP0478.
NMPDR	fig|262316.1.peg.478.
PATRIC	17993338. VBIMycAvi108102_0509.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016491.
HOGENOM	HBG327651.
OMA	DFDALNM.
ProtClustDB	PRK00260.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_MYCPA

Accession

Primary (citable) accession number: Q743W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program