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Q743L8 (OTSA_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Trehalose-phosphate synthase
Short name=TPS
EC=2.4.1.-
Alternative name(s):
Trehalose-6-phosphate synthase
Gene names
Name:otsA
Ordered Locus Names:MAP_0573c
OrganismMycobacterium paratuberculosis [Complete proteome] [HAMAP]
Taxonomic identifier1770 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length492 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6-phosphate and nucleoside diphosphate By similarity.

Catalytic activity

Nucleoside diphosphate-glucose + D-glucose 6-phosphate = trehalose 6-phosphate + nucleoside diphosphate.

Pathway

Glycan biosynthesis; trehalose biosynthesis.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtrehalose biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Molecular functiontransferase activity, transferring glycosyl groups

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 492492Trehalose-phosphate synthase
PRO_0000348909

Regions

Region45 – 462NDP-glucose binding By similarity
Region400 – 4045NDP-glucose binding By similarity

Sites

Binding site251Glucose-6-phosphate By similarity
Binding site1011Glucose-6-phosphate By similarity
Binding site1551Glucose-6-phosphate By similarity
Binding site2971NDP-glucose By similarity
Binding site3021NDP-glucose By similarity
Binding site3351Glucose-6-phosphate By similarity
Site1101Involved in alpha anomer selectivity By similarity
Site1801Involved in alpha anomer selectivity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q743L8 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 7CBAEC3BDA3C2291

FASTA49255,204
        10         20         30         40         50         60 
MAPGGGRGSK TAGYGNSDFV VVANRLPVDQ ERLPDGSTAW KRSPGGLVTA LEPLLRRQRG 

        70         80         90        100        110        120 
AWVGWPGIVD EDVDHEDDPI VQDDLELRPV KLSADDVAEY YEGFSNATLW PLYHDVIVKP 

       130        140        150        160        170        180 
IYHREWWDRY VAVNRRFAEA TSRAAARGAT VWVQDYQLQL VPAMLRELRP DLTIGFFLHI 

       190        200        210        220        230        240 
PFPPVELFMQ LPWRTEIVKG LLGADLVGFH LTGGAQNFLF LSRRLIGANT SRGAVGMRSR 

       250        260        270        280        290        300 
YGEVELESRV VRVGAFPISI DSTALDQTAR HRDIRRRARE IRAELGNPRK VLLGVDRLDY 

       310        320        330        340        350        360 
TKGIDVRLKA FSELLAEGRA KRDDTVLVQL ATPSRERVDS YQQLRNDIER QVGHINGEYG 

       370        380        390        400        410        420 
EVGHPVVHYL HRPVPRNELI AFFVAADVML VTPLRDGMNL VAKEYVACRS DLGGALVLSE 

       430        440        450        460        470        480 
FTGAAAELRQ AYLVNPHDLE GVKDTVEAAL NQSVEEGRRR MRSLRRQVLA HDVDRWARSF 

       490 
LDALAESGPR DG

« Hide

References

[1]"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016958 Genomic DNA. Translation: AAS02890.1.
RefSeqNP_959507.1. NC_002944.2.

3D structure databases

HSSPHSSP built from PDB template 1GZ5 based on UniProtKB P31677.
ProteinModelPortalQ743L8.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000039374; EBMYCP00000037763; EBMYCG00000039369.
GeneID2721288.
GenomeReviewsGene locus MAP_0573c in contig AE016958_GR.
KEGGmpa:MAP0573c.
NMPDRfig|262316.1.peg.573.
PATRIC17993533. VBIMycAvi108102_0606.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000016525.
HOGENOMHBG559076.
OMAEYGRKEM.
ProtClustDBCLSK872185.

Family and domain databases

InterProIPR001830. Glyco_trans_20.
[Graphical view]
KOK00697.
PfamPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameOTSA_MYCPA
AccessionPrimary (citable) accession number: Q743L8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: July 5, 2004
Last modified: January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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