Reviewed, UniProtKB/Swiss-Prot Q742Z0 (MHPB_MYCPA)

Names and origin

Protein names

Recommended name:
    2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
    EC=1.13.11.16

Gene names

Name:	mhpB
Ordered Locus Names:	MAP_0695

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity.
Catalytic activity	3-(2,3-dihydroxyphenyl)propanoate + O(2) = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. 2,3-dihydroxicinnamic acid + O(2) = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate.
Cofactor	Fe(2+) ion By similarity.
Pathway	Aromatic compound metabolism; 3-phenylpropionic acid degradation.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2,3-dihydroxy-phenylpropionate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP 3-carboxyethylcatechol 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro ferrous iron binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase		PRO_0000337654
Sites
	Active site	115	1	Proton donor By similarity
	Active site	179	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q742Z0-1 [UniParc]. Last modified May 20, 2008. Version 2. Checksum: 49A3010C87232A85	FASTA	312	33,150
	10 20 30 40 50 60 MALALCCMSH SPLLNLPGPS RDLLDDIDAA IARARGFVED YDPQLVVIFS PDHYNGFFYK 70 80 90 100 110 120 VMPPFCIGSS ASGVGDYGTH AGPLDVPEKL AVDCAQAVLD AGVDIAVSAS MDVDHGTVQP 130 140 150 160 170 180 LEKLFGTAIS RPVIPVFVNA IGVPLGPMHR CRALGAAVGT YLATLDKRVL VMGSGGLSHS 190 200 210 220 230 240 PPVPTLATAP EPVLQRIVHG APMTAEQRQA RQAAVIDAAR SFAAGDSALQ PLNPTWDHRF 250 260 270 280 290 300 LEIIDRGSLS ELDRWSNSFV THEGGGSAHE IRTWVAAFAA LQAAGPYETT MRYYTPAPEL 310 IAGFAIRTAR PK

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016958 Genomic DNA. Translation: AAS03012.1. Different initiation.
RefSeq	NP_959629.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2720204.
GenomeReviews	Gene locus MAP_0695 in contig AE016958_GR.
KEGG	mpa:MAP0695.
NMPDR	fig|262316.1.peg.695.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q742Z0.
Family and domain databases
HAMAP	MF_01653. [Tree]
InterPro	IPR004183. Xdiol_dOase_3B. [Graphical view]
Gene3D	G3DSA:3.40.830.10. Xdiol_dOase_3B. 1 hit.
Pfam	PF02900. LigB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MHPB_MYCPA

Accession

Primary (citable) accession number: Q742Z0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	May 20, 2008
Last modified:	November 25, 2008
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)