Q741W1 (ISPE_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	MAP_0976

Organism

Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10) [Complete proteome] [HAMAP]

Taxonomic identifier

262316 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC) › Mycobacterium avium › Mycobacterium paratuberculosis

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP-Rule MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP-Rule MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP-Rule MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: UniProtKB-UniPathway terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP-Rule MF_00061		PRO_0000189234
Regions
	Nucleotide binding	108 – 118	11	ATP Potential
Sites
	Active site	23	1	By similarity
	Active site	150	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q741W1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9DB2627B6C9CAFA7	FASTA	316	32,460
	10 20 30 40 50 60 MSDGNTAAAW VPTGSVTVRV PGKVNLYLAV GDRREDGYHE LTTIFQAVSL LDEVTVRNAD 70 80 90 100 110 120 VLSLDIVGEG ADKLPTDERN LAWQAAELMA DHVGRAPDVS IMIDKSIPVA GGMAGGSADA 130 140 150 160 170 180 AAVLVAMNSL WELNVPRRDL RMLAARLGSD VPFALHGGTA LGTGRGEELA TVLSRNTFHW 190 200 210 220 230 240 VLAFADGELL TRKVFAELDR LRRAGDPPRL PGPGPVLAAL AAGDADQLAP LLGNEMQAAA 250 260 270 280 290 300 VSLNPGLRRT LRAGVQAGAL AGIVSGSGPT CAFLCPSAAA AVDVGTEVSG VGVCRTVRVA 310 SGPVAGARVV PAPTEV

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS03293.1.
RefSeq	NP_959910.1. NC_002944.2.
3D structure databases
ProteinModelPortal	Q741W1.
ModBase	Search...
Protein-protein interaction databases
STRING	262316.MAP0976.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAS03293; AAS03293; MAP_0976.
GeneID	2721469.
KEGG	mpa:MAP0976.
PATRIC	17994375. VBIMycAvi108102_1020.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
KO	K00919.
OMA	GYHELNT.
ProtClustDB	PRK03188.
Enzyme and pathway databases
UniPathway	UPA00056; UER00094.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00061. IspE.
InterPro	IPR013750. GHMP_kinase_C_dom. IPR006204. GHMP_kinase_N_dom. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
PANTHER	PTHR20861:SF2. PTHR20861:SF2. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_MYCPA

Accession

Primary (citable) accession number: Q741W1

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program