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Q740U6

- SYI_MYCPA

UniProt

Q740U6 - SYI_MYCPA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei634 – 6341ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMAVI262316:GCQR-1266-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:MAP_1246
    OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
    Taxonomic identifieri262316 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
    ProteomesiUP000000580: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10531053Isoleucine--tRNA ligasePRO_0000098549Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi262316.MAP1246.

    Structurei

    3D structure databases

    ProteinModelPortaliQ740U6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi69 – 7911"HIGH" regionAdd
    BLAST
    Motifi631 – 6355"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    KOiK01870.
    OMAiENRDWFE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q740U6-1 [UniParc]FASTAAdd to Basket

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    MTDNLGFQAD REAQPADSVQ TYPKPAGGAP DFPALELEVL DYWARDDTFR     50
    ASIARRDGAE EYVFYDGPPF ANGLPHYGHL LTGYVKDIVP RYRTMRGNKV 100
    ERRFGWDTHG LPAELEVERQ LGITDKSQID DMGIAAFNDA CRESVLRYTD 150
    EWRAYVTRQA RWVDFDHDYK TLDLPYMESV IWAFKQLWDK GLAYEGYRVL 200
    PYCWRDETPL SNHELRMDDD VYPSRQDPAL TVGFKVVGGE LDGAHLLIWT 250
    TTPWTLPSNL AVAVHPEVTY VQVRSGDRRF VLAQPRLAAY ARELGPEPEI 300
    LGSYRGAELL GTRYLPPFPY FMDNPNAFRV LAGEFVTTED GTGIVHLAPA 350
    YGEDDMAVAD AAGIAPVTPV DSNGRFDAAV PDYQGLNVFD ANPQIIWDLK 400
    NGSGAAAANA PVLLRHETYE HPYPHCWRCR NPLIYRAVSS WFVAVTRFRD 450
    RMVELNQQIT WYPEHVKDGQ FGKWLAGARD WSISRNRYWG TPIPVWKSDD 500
    PAYPRIDVYG SLDELERDFG VRPDNLHRPY IDELTRPNPD DPSGRSTMRR 550
    IPDVLDVWFD SGSMPYAQVH YPFENRDWFE THYPGDFIVE YIGQTRGWFY 600
    TLHVLATALF DRPAFKTCVA HGIVLGSDGQ KMSKSLRNYP DVSEVFDRDG 650
    SDAMRWFLMA SPILRGGNLI VTEQGIRDGV RQVLLPFWNA YSFLALYAPK 700
    IGTWRTDSPQ VLDRYILAKL AELRDDLTRE MDACDISRAC EQLRQFTEAL 750
    TNWYVRRSRS RFWSEDADAI DTLHTVLEVT ARLAAPLLPS VTELVWRGLT 800
    GGRSVHLTDW PRSGELPADP GLVAAMDQVR EVCSAASSLR KAKKLRVRLP 850
    LPKLTVAVEN PQHLAPFVDL IADELNVKSV ELTDAIDTYG TFELTVNARV 900
    AGPRLGKDVQ AAIKAVKAGE GVVNADGTLT AGPAVLRPEE YSSRLVAADP 950
    EFTAALPNGA GLVVLDGTVT PELEAEGWAK DRIRELQELR KSTGLDVSDR 1000
    ISVVMSVPAG RAEWARTHRD LIAGEILATR FEFGEPADPV AIGDGVRVSI 1050
    SKV 1053
    Length:1,053
    Mass (Da):118,305
    Last modified:July 5, 2004 - v1
    Checksum:i0F210268429438E0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016958 Genomic DNA. Translation: AAS03563.1.
    RefSeqiNP_960180.1. NC_002944.2.

    Genome annotation databases

    EnsemblBacteriaiAAS03563; AAS03563; MAP_1246.
    GeneIDi2719583.
    KEGGimpa:MAP1246.
    PATRICi17994959. VBIMycAvi108102_1310.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016958 Genomic DNA. Translation: AAS03563.1 .
    RefSeqi NP_960180.1. NC_002944.2.

    3D structure databases

    ProteinModelPortali Q740U6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 262316.MAP1246.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAS03563 ; AAS03563 ; MAP_1246 .
    GeneIDi 2719583.
    KEGGi mpa:MAP1246.
    PATRICi 17994959. VBIMycAvi108102_1310.

    Phylogenomic databases

    eggNOGi COG0060.
    KOi K01870.
    OMAi ENRDWFE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci MAVI262316:GCQR-1266-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
      Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
      Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-968 / K-10.

    Entry informationi

    Entry nameiSYI_MYCPA
    AccessioniPrimary (citable) accession number: Q740U6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3