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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei634 – 6341ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMAVI262316:GCQR-1266-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:MAP_1246
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
ProteomesiUP000000580 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10531053Isoleucine--tRNA ligasePRO_0000098549Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi262316.MAP1246.

Structurei

3D structure databases

ProteinModelPortaliQ740U6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi69 – 7911"HIGH" regionAdd
BLAST
Motifi631 – 6355"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q740U6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDNLGFQAD REAQPADSVQ TYPKPAGGAP DFPALELEVL DYWARDDTFR
60 70 80 90 100
ASIARRDGAE EYVFYDGPPF ANGLPHYGHL LTGYVKDIVP RYRTMRGNKV
110 120 130 140 150
ERRFGWDTHG LPAELEVERQ LGITDKSQID DMGIAAFNDA CRESVLRYTD
160 170 180 190 200
EWRAYVTRQA RWVDFDHDYK TLDLPYMESV IWAFKQLWDK GLAYEGYRVL
210 220 230 240 250
PYCWRDETPL SNHELRMDDD VYPSRQDPAL TVGFKVVGGE LDGAHLLIWT
260 270 280 290 300
TTPWTLPSNL AVAVHPEVTY VQVRSGDRRF VLAQPRLAAY ARELGPEPEI
310 320 330 340 350
LGSYRGAELL GTRYLPPFPY FMDNPNAFRV LAGEFVTTED GTGIVHLAPA
360 370 380 390 400
YGEDDMAVAD AAGIAPVTPV DSNGRFDAAV PDYQGLNVFD ANPQIIWDLK
410 420 430 440 450
NGSGAAAANA PVLLRHETYE HPYPHCWRCR NPLIYRAVSS WFVAVTRFRD
460 470 480 490 500
RMVELNQQIT WYPEHVKDGQ FGKWLAGARD WSISRNRYWG TPIPVWKSDD
510 520 530 540 550
PAYPRIDVYG SLDELERDFG VRPDNLHRPY IDELTRPNPD DPSGRSTMRR
560 570 580 590 600
IPDVLDVWFD SGSMPYAQVH YPFENRDWFE THYPGDFIVE YIGQTRGWFY
610 620 630 640 650
TLHVLATALF DRPAFKTCVA HGIVLGSDGQ KMSKSLRNYP DVSEVFDRDG
660 670 680 690 700
SDAMRWFLMA SPILRGGNLI VTEQGIRDGV RQVLLPFWNA YSFLALYAPK
710 720 730 740 750
IGTWRTDSPQ VLDRYILAKL AELRDDLTRE MDACDISRAC EQLRQFTEAL
760 770 780 790 800
TNWYVRRSRS RFWSEDADAI DTLHTVLEVT ARLAAPLLPS VTELVWRGLT
810 820 830 840 850
GGRSVHLTDW PRSGELPADP GLVAAMDQVR EVCSAASSLR KAKKLRVRLP
860 870 880 890 900
LPKLTVAVEN PQHLAPFVDL IADELNVKSV ELTDAIDTYG TFELTVNARV
910 920 930 940 950
AGPRLGKDVQ AAIKAVKAGE GVVNADGTLT AGPAVLRPEE YSSRLVAADP
960 970 980 990 1000
EFTAALPNGA GLVVLDGTVT PELEAEGWAK DRIRELQELR KSTGLDVSDR
1010 1020 1030 1040 1050
ISVVMSVPAG RAEWARTHRD LIAGEILATR FEFGEPADPV AIGDGVRVSI

SKV
Length:1,053
Mass (Da):118,305
Last modified:July 5, 2004 - v1
Checksum:i0F210268429438E0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS03563.1.
RefSeqiNP_960180.1. NC_002944.2.
WP_003877692.1. NC_002944.2.

Genome annotation databases

EnsemblBacteriaiAAS03563; AAS03563; MAP_1246.
KEGGimpa:MAP1246.
PATRICi17994959. VBIMycAvi108102_1310.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS03563.1.
RefSeqiNP_960180.1. NC_002944.2.
WP_003877692.1. NC_002944.2.

3D structure databases

ProteinModelPortaliQ740U6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262316.MAP1246.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS03563; AAS03563; MAP_1246.
KEGGimpa:MAP1246.
PATRICi17994959. VBIMycAvi108102_1310.

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiIPLPIWR.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciMAVI262316:GCQR-1266-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
    Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
    Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-968 / K-10.

Entry informationi

Entry nameiSYI_MYCPA
AccessioniPrimary (citable) accession number: Q740U6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: June 24, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.