Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q740Q9 (BIOB_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length344 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 344344Biotin synthase HAMAP-Rule MF_01694
PRO_0000381481

Sites

Metal binding801Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding841Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding871Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1231Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1561Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2151Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2851Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q740Q9 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: AE80DB3CE6BD706B

FASTA34437,083
        10         20         30         40         50         60 
MTQAATRPTA EIGNEDILAV ARRQVLEGGQ GLSRDQVLQV LRLPDDRLDD LLALAHEVRM 

        70         80         90        100        110        120 
RWCGPEVEVE GIISLKTGGC PEDCHFCSQS GLFASPVRSA RLDIPSLVEA AKQTAKSGAT 

       130        140        150        160        170        180 
EFCIVAAVRG PDERLLAQVA AGIEAVRNEV EINIACSLGM LTAEQVEQLA EMGVHRYNHN 

       190        200        210        220        230        240 
LETARSFFPN VVTTHTWEER WDTLSMVRDA GMEVCCGGIL GMGETLEQRA EFAAELAELG 

       250        260        270        280        290        300 
PDEVPLNFLN PRPGTPFGDL EVMPATEALK SVAAFRLALP RTMLRFAGGR EITLGDLGAK 

       310        320        330        340 
KGILGGINAV IVGNYLTTLG RPAESDLELL DDLQMPLKGL NASL 

« Hide

References

[1]"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016958 Genomic DNA. Translation: AAS03600.1.
RefSeqNP_960217.1. NC_002944.2.

3D structure databases

ProteinModelPortalQ740Q9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING262316.MAP1283.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS03600; AAS03600; MAP_1283.
GeneID2718143.
KEGGmpa:MAP1283.
PATRIC17995043. VBIMycAvi108102_1352.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
KOK01012.
OMAETHHNID.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycMAVI262316:GCQR-1303-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_MYCPA
AccessionPrimary (citable) accession number: Q740Q9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways