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Q740L5 (Q740L5_MYCPA) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
UvrABC system protein B HAMAP-Rule MF_00204
Short name=Protein UvrB HAMAP-Rule MF_00204
Alternative name(s):
Excinuclease ABC subunit B HAMAP-Rule MF_00204
Gene names
Name:uvrB HAMAP-Rule MF_00204 EMBL AAS03652.1
Ordered Locus Names:MAP_1335
OrganismMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10) [Complete proteome] [HAMAP]
Taxonomic identifier262316 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC) ›

Protein attributes

Sequence length722 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage By similarity. HAMAP-Rule MF_00204 SAAS SAAS024759

Subunit structure

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex By similarity. RuleBase RU003587 HAMAP-Rule MF_00204 SAAS SAAS024759

Subcellular location

Cytoplasm By similarity RuleBase RU003587 HAMAP-Rule MF_00204 SAAS SAAS024759.

Domain

The beta-hairpin motif is involved in DNA binding By similarity. HAMAP-Rule MF_00204

Sequence similarities

Belongs to the UvrB family. RuleBase RU003587 HAMAP-Rule MF_00204

Contains 1 UVR domain. RuleBase RU003587 SAAS SAAS024759 HAMAP-Rule MF_00204

Contains 1 helicase ATP-binding domain. SAAS SAAS024759 HAMAP-Rule MF_00204

Contains 1 helicase C-terminal domain. HAMAP-Rule MF_00204 SAAS SAAS024759

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain53 – 213161Helicase ATP-binding By similarity HAMAP-Rule MF_00204
Domain457 – 623167Helicase C-terminal By similarity HAMAP-Rule MF_00204
Domain677 – 71236UVR By similarity HAMAP-Rule MF_00204
Nucleotide binding66 – 738ATP By similarity HAMAP-Rule MF_00204
Motif119 – 14224Beta-hairpin By similarity HAMAP-Rule MF_00204

Sequences

Sequence LengthMass (Da)Tools
Q740L5 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 208339B4571069BC

FASTA72280,921
        10         20         30         40         50         60 
MAFATEHPVV AHSEYRPAEE AVEGLVRAGG RFEVVSPHAP AGDQPAAIDE LERRIRAGER 

        70         80         90        100        110        120 
DVVLLGATGT GKSATTAWLI ERLQRPTLVM APNKTLAAQL ANELREMLPH NAVEYFVSYY 

       130        140        150        160        170        180 
DYYQPEAYIA QTDTYIEKDS SINDDVERLR HSATSSLLSR RDVVVVASVS CIYGLGTPQS 

       190        200        210        220        230        240 
YLDRSVELRV GTEVPRDALL RLLVDVQYTR NDLSFTRGSF RVRGDTVEII PSYEELAVRI 

       250        260        270        280        290        300 
EFFGDEIEAL YYLHPLTGDV IRQVDSLRIF PATHYVAGPE RMAHAISTIE QELAERLAEL 

       310        320        330        340        350        360 
EGQGKLLEAQ RLRMRTNYDI EMMRQVGFCS GIENYSRHID GRGPGSPPAT LLDYFPEDFL 

       370        380        390        400        410        420 
LVIDESHVTV PQIGGMYEGD MSRKRNLVEY GFRLPSACDN RPLTWEEFAD RIGQTVYLSA 

       430        440        450        460        470        480 
TPGPYELSQT GGEFVEQVIR PTGLVDPKVV VKPTKGQIDD LIGEIRKRTE ADERVLVTTL 

       490        500        510        520        530        540 
TKKMAEDLTD YLLEMGIRVR YLHSEVDTLR RVELLRQLRL GEYDVLVGIN LLREGLDLPE 

       550        560        570        580        590        600 
VSLVAILDAD KEGFLRSARS LIQTIGRAAR NVSGEVHMYA DTITESMKEA IDETERRRAK 

       610        620        630        640        650        660 
QIAYNEAHGI DPQPLRKKIA DILDQVYREA DDTDTVEIGS GRSMSRGRRA QGEPGRAVSA 

       670        680        690        700        710        720 
GIVEGRDTTN MPRAELADLI KDLTAQMMAA ARDLQFELAA RFRDEIADLK KELRGMDAAG 


LK

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References

[1]"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016958 Genomic DNA. Translation: AAS03652.1.
RefSeqNP_960269.1. NC_002944.2.

3D structure databases

ProteinModelPortalQ740L5.
ModBaseSearch...

Protein-protein interaction databases

STRING262316.MAP1335.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS03652; AAS03652; MAP_1335.
GeneID2720724.
KEGGmpa:MAP1335.
PATRIC17995157. VBIMycAvi108102_1408.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK03702.
OMACIYGLGI.
ProtClustDBPRK05298.

Family and domain databases

Gene3D4.10.860.10. 1 hit.
HAMAPMF_00204. UvrB.
InterProIPR006935. Helicase/UvrB_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR001943. UVR_dom.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
[Graphical view]
PANTHERPTHR24029. PTHR24029. 1 hit.
PfamPF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF46600. UvrB_C. 1 hit.
TIGRFAMsTIGR00631. uvrb. 1 hit.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ740L5_MYCPA
AccessionPrimary (citable) accession number: Q740L5
Entry history
Integrated into UniProtKB/TrEMBL: July 5, 2004
Last sequence update: July 5, 2004
Last modified: May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info