Q740J1 (SYFA_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	MAP_1359

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_0000231995
Sites
	Metal binding	265	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q740J1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5FB9EBE9F21AFA13	FASTA	347	37,935
	10 20 30 40 50 60 MGDQPVDLSE TALAEAVGAA RQAFARAGDL DALARLKTEH LGDRAPLALA RQALGGVPKD 70 80 90 100 110 120 QRADAGRRVN AARAQAQQAY DERLDVLRAE RDAAVLVAER IDVTLPSTRQ PVGARHPITI 130 140 150 160 170 180 LAEHIADTFI AMGWELAEGP EVEAEQFNFD ALNFPADHPA RSEQDTFYIA PEGSRQLLRT 190 200 210 220 230 240 HTSPVQVRTL LARELPVYVI SIGRTFRTDE LDATHTPVFH QVEGLAVDRG LSMAHLRGTL 250 260 270 280 290 300 DAFARAEFGP QARTRIRPHF FPFTEPSAEV DVWFVGKKGG AGWVEWGGCG MVHPNVLRAA 310 320 330 340 GIDPDVYSGF AFGMGLERTL QFRNGIPDMR DMVEGDVRFL LPFGVGA

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS03676.1.
RefSeq	NP_960293.1. NC_002944.2.
3D structure databases
ProteinModelPortal	Q740J1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000036907; EBMYCP00000035296; EBMYCG00000036902.
GeneID	2719916.
GenomeReviews	Gene locus MAP_1359 in contig AE016958_GR.
KEGG	mpa:MAP1359.
NMPDR	fig|262316.1.peg.1359.
PATRIC	17995207. VBIMycAvi108102_1433.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016426.
HOGENOM	HBG284353.
OMA	FRASYFP.
ProtClustDB	PRK00488.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_MYCPA

Accession

Primary (citable) accession number: Q740J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program