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Protein

Ornithine carbamoyltransferase

Gene

argF

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.UniRule annotation

Pathway:iL-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei7 – 71Carbamoyl phosphateUniRule annotation
Sitei28 – 281Important for structural integrityUniRule annotation
Binding sitei78 – 781Carbamoyl phosphateUniRule annotation
Binding sitei102 – 1021Carbamoyl phosphateUniRule annotation
Sitei142 – 1421Important for structural integrityUniRule annotation
Binding sitei161 – 1611OrnithineBy similarity
Binding sitei225 – 2251OrnithineBy similarity
Binding sitei275 – 2751Carbamoyl phosphateUniRule annotation
Binding sitei293 – 2931Carbamoyl phosphateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

BioCyciMAVI262316:GCQR-1386-MONOMER.
UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferaseUniRule annotation (EC:2.1.3.3UniRule annotation)
Short name:
OTCaseUniRule annotation
Gene namesi
Name:argFUniRule annotation
Ordered Locus Names:MAP_1365
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
ProteomesiUP000000580 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Ornithine carbamoyltransferasePRO_0000112952Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi262316.MAP1365.

Structurei

3D structure databases

ProteinModelPortaliQ740I5.
SMRiQ740I5. Positions 4-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni51 – 555Carbamoyl phosphate bindingUniRule annotation
Regioni129 – 1324Carbamoyl phosphate bindingUniRule annotation
Regioni229 – 2302Ornithine bindingUniRule annotation
Regioni264 – 2674Carbamoyl phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATCase/OTCase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0078.
KOiK00611.
OMAiVWISMGQ.
OrthoDBiEOG690MGV.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q740I5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPRHFLRDD DLSPAEQAEV LALAAELKKD PFSARPLEGP RGVAVLFDKN
60 70 80 90 100
STRTRFSFEV GIAQLGGHAV VVDARSTQLG RDETLEDTAR VLSRYVEAIV
110 120 130 140 150
WRTFEQQRLE AMAGAATVPV INALSDEFHP CQMLADLQAI AEHKGSLSGL
160 170 180 190 200
RMCYLGDGAN NMAHSLMLGG VTAGIHVTIA APDGFTPAPE FVAAARRRAE
210 220 230 240 250
STGATVTLTT DARAAARGVD VLVTDTWTSM GQEDDGLDRR TPFWPYQLNA
260 270 280 290 300
DLVSLADPEA IVLHCLPAHR GEEITDEVMD GPSSVVWDEA ENRLHAQKAL

LTWLLERQS
Length:309
Mass (Da):33,595
Last modified:July 5, 2004 - v1
Checksum:i7500E6BE03398101
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS03682.1.
RefSeqiWP_003877772.1. NC_002944.2.

Genome annotation databases

EnsemblBacteriaiAAS03682; AAS03682; MAP_1365.
GeneIDi2720213.
KEGGimpa:MAP1365.
PATRICi17995219. VBIMycAvi108102_1439.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS03682.1.
RefSeqiWP_003877772.1. NC_002944.2.

3D structure databases

ProteinModelPortaliQ740I5.
SMRiQ740I5. Positions 4-307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262316.MAP1365.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS03682; AAS03682; MAP_1365.
GeneIDi2720213.
KEGGimpa:MAP1365.
PATRICi17995219. VBIMycAvi108102_1439.

Phylogenomic databases

eggNOGiCOG0078.
KOiK00611.
OMAiVWISMGQ.
OrthoDBiEOG690MGV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.
BioCyciMAVI262316:GCQR-1386-MONOMER.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
    Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
    Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-968 / K-10.

Entry informationi

Entry nameiOTC_MYCPA
AccessioniPrimary (citable) accession number: Q740I5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.