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Q73ZQ2 (MASZ_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate synthase G
EC=2.3.3.9
Gene names
Name:glcB
Ordered Locus Names:MAP_1549c
OrganismMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10) [Complete proteome] [HAMAP]
Taxonomic identifier262316 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC) ›

Protein attributes

Sequence length734 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641

Pathway

Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
   Biological processGlyoxylate bypass
Tricarboxylic acid cycle
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglyoxylate cycle

Inferred from electronic annotation. Source: HAMAP

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmalate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 734734Malate synthase G HAMAP-Rule MF_00641
PRO_1000056906

Sites

Active site3411Proton acceptor By similarity
Active site6381Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q73ZQ2 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: A9B428116E736C30

FASTA73480,345
        10         20         30         40         50         60 
MTDRVSAGNL RVARVLYDFV NDEALPGTDI DPDSFWAGVD KVVTDLTPRN QELLRRRDEL 

        70         80         90        100        110        120 
QAQIDKWHRQ RVIEPLDIDA YRDFLIEIGY LLPEPEDFTI TTSGVDDEIT TTAGPQLVVP 

       130        140        150        160        170        180 
VLNARFALNA ANARWGSLYD ALYGTDVIPE TDGAEKGSSY NKVRGDKVIA YARNFLDQAV 

       190        200        210        220        230        240 
PLESGSWADA TGLSVEDGRL QVATADGSVG LAEPAKFAGY TGQLGSPDWS VLLVNHGLHI 

       250        260        270        280        290        300 
EILIDPQSPV GKTDRAGIKD VVLESAVTTI MDFEDSVAAV DADDKVLGYR NWLGLNKGDL 

       310        320        330        340        350        360 
SEEVSKDGKT FTRVLNADRT YTTPDGQGEL TLPGRSLLFV RNVGHLMTND AIVWSDGDEE 

       370        380        390        400        410        420 
KEVFEGIMDA LFTGLTAIHG LKTGEANGPL QNSRTGSIYI VKPKMHGPDE VAFTCELFSR 

       430        440        450        460        470        480 
VEDVLGLPQG TLKIGIMDEE RRTTVNLKAC IKAAADRVVF INTGFLDRTG DEIHTSMEAG 

       490        500        510        520        530        540 
PMIRKGAMKN TTWIKAYEDA NVDIGLAAGF KGKAQIGKGM WAMTELMADM VEQKIGQPKA 

       550        560        570        580        590        600 
GATTAWVPSP TAATLHAMHY HYVDVGAVQE ELAGKKRTTI EQLLTIPLAK ELAWAPEEIR 

       610        620        630        640        650        660 
EEVDNNCQSI LGYVVRWVAQ GVGCSKVPDI HDVALMEDRA TLRISSQLLA NWLRHGVITE 

       670        680        690        700        710        720 
EDVRASLERM APLVDAQNAK DAAYQPMAPN FDDSLAFLAA QDLILTGTQQ PNGYTEPILH 

       730 
RRRREVKARA AQSN

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References

[1]"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016958 Genomic DNA. Translation: AAS03866.1.
RefSeqNP_960483.1. NC_002944.2.

3D structure databases

HSSPHSSP built from PDB template 1N8W based on UniProtKB P0A5J4.
ProteinModelPortalQ73ZQ2.
SMRQ73ZQ2. Positions 5-732.
ModBaseSearch...

Protein-protein interaction databases

STRING262316.MAP1549c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS03866; AAS03866; MAP_1549c.
GeneID2719627.
KEGGmpa:MAP1549c.
PATRIC17995624. VBIMycAvi108102_1640.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG2225.
KOK01638.
OMAVVKPKMH.
ProtClustDBPRK02999.

Enzyme and pathway databases

UniPathwayUPA00703; UER00720.

Family and domain databases

Gene3D2.170.170.11. 2 hits.
HAMAPMF_00641. Malate_synth_G.
InterProIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PANTHERPTHR21631:SF1. PTHR21631:SF1. 1 hit.
PfamPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMSSF51645. Malat_synth_like. 1 hit.
TIGRFAMsTIGR01345. malate_syn_G. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMASZ_MYCPA
AccessionPrimary (citable) accession number: Q73ZQ2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 5, 2004
Last modified: May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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