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Protein

Malate synthase G

Gene

glcB

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei276Acetyl-CoAUniRule annotation1
Binding sitei313Acetyl-CoAUniRule annotation1
Active sitei341Proton acceptorUniRule annotation1
Binding sitei341GlyoxylateUniRule annotation1
Metal bindingi439MagnesiumUniRule annotation1
Binding sitei439GlyoxylateUniRule annotation1
Metal bindingi467MagnesiumUniRule annotation1
Binding sitei548Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei638Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciMAVI262316:G1G0E-1619-MONOMER
UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:MAP_1549c
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi
  • UP000000580 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000569061 – 734Malate synthase GAdd BLAST734

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei624Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi262316.MAP1549c

Structurei

3D structure databases

ProteinModelPortaliQ73ZQ2
SMRiQ73ZQ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni464 – 467Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3 Bacteria
COG2225 LUCA
KOiK01638
OMAiTEPVLHA
OrthoDBiPOG091H05P0

Family and domain databases

CDDicd00728 malate_synt_G, 1 hit
Gene3Di2.170.170.11, 1 hit
HAMAPiMF_00641 Malate_synth_G, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR023310 Malate_synth_G_beta_sub_dom
IPR001465 Malate_synthase
IPR006253 Malate_synthG
PANTHERiPTHR42739 PTHR42739, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01345 malate_syn_G, 1 hit

Sequencei

Sequence statusi: Complete.

Q73ZQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRVSAGNL RVARVLYDFV NDEALPGTDI DPDSFWAGVD KVVTDLTPRN
60 70 80 90 100
QELLRRRDEL QAQIDKWHRQ RVIEPLDIDA YRDFLIEIGY LLPEPEDFTI
110 120 130 140 150
TTSGVDDEIT TTAGPQLVVP VLNARFALNA ANARWGSLYD ALYGTDVIPE
160 170 180 190 200
TDGAEKGSSY NKVRGDKVIA YARNFLDQAV PLESGSWADA TGLSVEDGRL
210 220 230 240 250
QVATADGSVG LAEPAKFAGY TGQLGSPDWS VLLVNHGLHI EILIDPQSPV
260 270 280 290 300
GKTDRAGIKD VVLESAVTTI MDFEDSVAAV DADDKVLGYR NWLGLNKGDL
310 320 330 340 350
SEEVSKDGKT FTRVLNADRT YTTPDGQGEL TLPGRSLLFV RNVGHLMTND
360 370 380 390 400
AIVWSDGDEE KEVFEGIMDA LFTGLTAIHG LKTGEANGPL QNSRTGSIYI
410 420 430 440 450
VKPKMHGPDE VAFTCELFSR VEDVLGLPQG TLKIGIMDEE RRTTVNLKAC
460 470 480 490 500
IKAAADRVVF INTGFLDRTG DEIHTSMEAG PMIRKGAMKN TTWIKAYEDA
510 520 530 540 550
NVDIGLAAGF KGKAQIGKGM WAMTELMADM VEQKIGQPKA GATTAWVPSP
560 570 580 590 600
TAATLHAMHY HYVDVGAVQE ELAGKKRTTI EQLLTIPLAK ELAWAPEEIR
610 620 630 640 650
EEVDNNCQSI LGYVVRWVAQ GVGCSKVPDI HDVALMEDRA TLRISSQLLA
660 670 680 690 700
NWLRHGVITE EDVRASLERM APLVDAQNAK DAAYQPMAPN FDDSLAFLAA
710 720 730
QDLILTGTQQ PNGYTEPILH RRRREVKARA AQSN
Length:734
Mass (Da):80,345
Last modified:July 5, 2004 - v1
Checksum:iA9B428116E736C30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA Translation: AAS03866.1
RefSeqiWP_003876515.1, NC_002944.2

Genome annotation databases

EnsemblBacteriaiAAS03866; AAS03866; MAP_1549c
GeneIDi2719627
KEGGimpa:MAP_1549c

Similar proteinsi

Entry informationi

Entry nameiMASZ_MYCPA
AccessioniPrimary (citable) accession number: Q73ZQ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 5, 2004
Last modified: April 25, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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