Q73ZQ2 (MASZ_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	MAP_1549c

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	734 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 734	734	Malate synthase G HAMAP MF_00641		PRO_1000056906
Sites
	Active site	341	1	Proton acceptor By similarity
	Active site	638	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q73ZQ2 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: A9B428116E736C30	FASTA	734	80,345
	10 20 30 40 50 60 MTDRVSAGNL RVARVLYDFV NDEALPGTDI DPDSFWAGVD KVVTDLTPRN QELLRRRDEL 70 80 90 100 110 120 QAQIDKWHRQ RVIEPLDIDA YRDFLIEIGY LLPEPEDFTI TTSGVDDEIT TTAGPQLVVP 130 140 150 160 170 180 VLNARFALNA ANARWGSLYD ALYGTDVIPE TDGAEKGSSY NKVRGDKVIA YARNFLDQAV 190 200 210 220 230 240 PLESGSWADA TGLSVEDGRL QVATADGSVG LAEPAKFAGY TGQLGSPDWS VLLVNHGLHI 250 260 270 280 290 300 EILIDPQSPV GKTDRAGIKD VVLESAVTTI MDFEDSVAAV DADDKVLGYR NWLGLNKGDL 310 320 330 340 350 360 SEEVSKDGKT FTRVLNADRT YTTPDGQGEL TLPGRSLLFV RNVGHLMTND AIVWSDGDEE 370 380 390 400 410 420 KEVFEGIMDA LFTGLTAIHG LKTGEANGPL QNSRTGSIYI VKPKMHGPDE VAFTCELFSR 430 440 450 460 470 480 VEDVLGLPQG TLKIGIMDEE RRTTVNLKAC IKAAADRVVF INTGFLDRTG DEIHTSMEAG 490 500 510 520 530 540 PMIRKGAMKN TTWIKAYEDA NVDIGLAAGF KGKAQIGKGM WAMTELMADM VEQKIGQPKA 550 560 570 580 590 600 GATTAWVPSP TAATLHAMHY HYVDVGAVQE ELAGKKRTTI EQLLTIPLAK ELAWAPEEIR 610 620 630 640 650 660 EEVDNNCQSI LGYVVRWVAQ GVGCSKVPDI HDVALMEDRA TLRISSQLLA NWLRHGVITE 670 680 690 700 710 720 EDVRASLERM APLVDAQNAK DAAYQPMAPN FDDSLAFLAA QDLILTGTQQ PNGYTEPILH 730 RRRREVKARA AQSN

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS03866.1.
RefSeq	NP_960483.1. NC_002944.2.
3D structure databases
HSSP	HSSP built from PDB template 1N8W based on UniProtKB P0A5J4.
ProteinModelPortal	Q73ZQ2.
SMR	Q73ZQ2. Positions 5-732.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000037379; EBMYCP00000035768; EBMYCG00000037374.
GeneID	2719627.
GenomeReviews	Gene locus MAP_1549c in contig AE016958_GR.
KEGG	mpa:MAP1549c.
NMPDR	fig|262316.1.peg.1549.
PATRIC	17995624. VBIMycAvi108102_1640.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00070000032288.
HOGENOM	HBG350005.
OMA	PKMHGPD.
ProtClustDB	PRK02999.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_MYCPA

Accession

Primary (citable) accession number: Q73ZQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program