Q73ZD5 (KATG_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	MAP_1668c

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 748	748	Catalase-peroxidase HAMAP MF_01961		PRO_0000354839
Sites
	Active site	114	1	Proton acceptor By similarity
	Metal binding	279	1	Iron (heme axial ligand) By similarity
	Site	110	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	113 ↔ 238		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-264) By similarity
	Cross-link	238 ↔ 264		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-113) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q73ZD5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4413057EF877C69F	FASTA	748	81,750
	10 20 30 40 50 60 MSSDTSASRP PQPDTRTASK SESENPAIPS PHPKSNAPLT NRDWWPNQID VSRLHPHVAE 70 80 90 100 110 120 ANPLGEDFDY AEEFAKLDVE ALKADVISVM TTSQDWWPAD YGHYGGLFIR MSWHAAGTYR 130 140 150 160 170 180 IHDGRGGGGQ GMQRFAPLNS WPDNVSLDKA RRLLWPVKKK YGNKISWADL IIFAGNCALE 190 200 210 220 230 240 SMGFKTFGFA FGREDVWEPE EILWGEEDEW LGTDKRYPGT GERELAQPYG ATTMGLIYVN 250 260 270 280 290 300 PEGPEGKPDP IAAAIDIRET FGRMAMNDEE TAALIVGGHS FGKTHGAGDA DLVGPEPEAA 310 320 330 340 350 360 PIEQQGLGWK SSHGTGVGKD AITSGLEVVW TPTPTKWDNT FLETLYGYEW ELTKSPAGAW 370 380 390 400 410 420 QFTAKDGAGA GTIPDPFGGP GRAPTMLVTD ISLRESPIYR DITRRWLDHP EELADAFAKA 430 440 450 460 470 480 WYKLLHRDMG LVSRFLGPWV PEPQLWQDPV PPVDHPLVDD NDVAALKDKV LASGLSVPQL 490 500 510 520 530 540 VKTAWSAAGS YRNTDKRGGA NGGRLRLQPQ RNWEANEPSE LDKVLPVLEK IQQDFNASAS 550 560 570 580 590 600 GGKKISLADL IVLAGSAAVE KAAKDAGYEI SVHFAPGRTD ASQESTDVDS FAVLEPRADG 610 620 630 640 650 660 FRNFARPGEK APLEQLLLER AYLLGVTGPE MTVLVGGLRA LGANHGGSKH GVFTDRPGAL 670 680 690 700 710 720 TNDFFVNLLD MGTEWKASET AENVYEGHDR ATGALKWTAT ANDLVFGSNS VLRALAEVYA 730 740 QDDNQGKFVE DFVAAWVKVM NNDRFDLK

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS03985.1.
RefSeq	NP_960602.1. NC_002944.2.
3D structure databases
HSSP	HSSP built from PDB template 2CCA based on UniProtKB Q50555.
ProteinModelPortal	Q73ZD5.
SMR	Q73ZD5. Positions 41-748.
ModBase	Search...
Protein family/group databases
PeroxiBase	2712. MavpCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000038293; EBMYCP00000036682; EBMYCG00000038288.
GeneID	2719240.
GenomeReviews	Gene locus MAP_1668c in contig AE016958_GR.
KEGG	mpa:MAP1668c.
NMPDR	fig|262316.1.peg.1668.
PATRIC	17995868. VBIMycAvi108102_1762.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000017549.
HOGENOM	HBG285610.
OMA	QGKFVED.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_MYCPA

Accession

Primary (citable) accession number: Q73ZD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program