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Q73YS9 (MSHC_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase
Short name=L-Cys:GlcN-Ins ligase
EC=6.3.1.13
Alternative name(s):
Mycothiol ligase
Short name=MSH ligase
Gene names
Name:mshC
Synonyms:cysS2
Ordered Locus Names:MAP_1876c
OrganismMycobacterium paratuberculosis [Complete proteome] [HAMAP]
Taxonomic identifier1770 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the ATP-dependent condensation of GlcN-Ins and L-cysteine to form L-Cys-GlcN-Ins By similarity. HAMAP MF_01697

Catalytic activity

1-O-(2-amino-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + L-cysteine + ATP = 1-O-(2-(L-cysteinamido)-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + AMP + diphosphate. HAMAP MF_01697

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_01697

Subunit structure

Monomer By similarity. HAMAP MF_01697

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MshC subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415L-cysteine:1D-myo-inositol 2-amino-2-deoxy-alpha-D-glucopyranoside ligase HAMAP MF_01697
PRO_0000159440

Regions

Region47 – 504Cysteinyl adenylate binding By similarity
Region85 – 873Cysteinyl adenylate binding By similarity
Region252 – 2543Cysteinyl adenylate binding By similarity
Motif49 – 5911"HIGH" region HAMAP MF_01697
Motif190 – 1956"ERGGDP" region HAMAP MF_01697
Motif292 – 2965"KMSKS" region HAMAP MF_01697

Sites

Metal binding471Zinc By similarity
Metal binding2341Zinc By similarity
Metal binding2591Zinc By similarity
Binding site621Cysteinyl adenylate By similarity
Binding site2301Cysteinyl adenylate By similarity
Binding site2861Cysteinyl adenylate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q73YS9 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: D7BE9F9DCCCEE206

FASTA41545,367
        10         20         30         40         50         60 
MRSWSSPQVP QLPGRGPELR LYDTSDRQVR PVAAGAGPGS AATMYVCGIT PYDATHLGHA 

        70         80         90        100        110        120 
ATYLAFDLIY RQWLDLGHDV HYVQNVTDVD DPLLERAARD GVDWRALAER EVSLFREDMA 

       130        140        150        160        170        180 
ALRILAPRDY VGATEAIADV VELVEKMLAS GAAYVVDGEF PDIYYRADAT LQFGYESGYD 

       190        200        210        220        230        240 
RETMLRLFAE RGGDPQRPGK TDALDALLWR AARPGEPSWP SPFGNGRPGW HVECAAIALS 

       250        260        270        280        290        300 
RIGSGLDIQG GGSDLIFPHH EFTAAHAECV RGERRFARHY VHAGMIGWDG HKMSKSRGNL 

       310        320        330        340        350        360 
VLVSQLRGRG VEPAAIRLGL LAGHYRGDRY WSDQVLDEAT ARLRRWRTAT ALPAGPDATD 

       370        380        390        400        410 
VIARVRQYLA DDLNTPKALA ALDGWTTDAL DYGGHDTAAP RLVASAVDAL LGVAL

« Hide

References

[1]"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE016958 Genomic DNA. Translation: AAS04193.1.
RefSeqNP_960810.1. NC_002944.2.

3D structure databases

ProteinModelPortalQ73YS9.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000038971; EBMYCP00000037360; EBMYCG00000038966.
GeneID2717833.
GenomeReviewsGene locus MAP_1876c in contig AE016958_GR.
KEGGmpa:MAP1876c.
NMPDRfig|262316.1.peg.1876.
PATRIC17996318. VBIMycAvi108102_1987.

Organism-specific databases

CMRSearch...

Phylogenomic databases

GeneTreeEBGT00050000016491.
HOGENOMHBG327651.
OMAALFREDM.
ProtClustDBPRK12418.

Family and domain databases

HAMAPMF_01697. MshC.
[Tree]
InterProIPR024909. Cys-tRNA/MSH_ligase.
IPR017812. Mycothiol_ligase_MshC.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK15526.
PANTHERPTHR10890. Cys_tRNA-synt_1a. 1 hit.
PfamPF01406. tRNA-synt_1e. 1 hit.
[Graphical view]
PRINTSPR00983. TRNASYNTHCYS.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR03447. Mycothiol_MshC. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMSHC_MYCPA
AccessionPrimary (citable) accession number: Q73YS9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: July 5, 2004
Last modified: January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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