Reviewed, UniProtKB/Swiss-Prot Q73XY8 (MBTG_MYCPA)

Names and origin

Protein names

Recommended name:
    L-lysine 6-monooxygenase mbtG
    EC=1.14.13.59
Alternative name(s):
    Lysine 6-N-hydroxylase
    Lysine N(6)-hydroxylase
    Lysine-N-oxygenase
    Mycobactin synthetase protein G

Gene names

Name:	mbtG
Ordered Locus Names:	MAP_2170c

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Flavoprotein monooxygenase required for N-hydroxylation of the two acylated lysine residues during mycobactin assembly, thus producing the hydroxamate groups necessary for iron sequestration. Is also able, but less efficiently, to hydroxylate L-lysine (non acylated) in vitro By similarity.
Catalytic activity	L-lysine + NADPH + O(2) = N(6)-hydroxy-L-lysine + NADP(+) + H(2)O.
Cofactor	FAD By similarity.
Pathway	Siderophore biosynthesis; mycobactin biosynthesis.
Sequence similarities	Belongs to the lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family.

Ontologies

Keywords
Biological process	Ion transport Iron transport Transport
Domain	Signal
Ligand	FAD Flavoprotein Iron NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	iron ion transport Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-lysine 6-monooxygenase (NADPH) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 428	408	L-lysine 6-monooxygenase mbtG		PRO_0000261310

Sequences

	Sequence		Length	Mass (Da)
	Q73XY8-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 44C8EE39A4AFA801	FASTA	428	46,532
	10 20 30 40 50 60 MSTLAILGAG AKAVAVAAKA SVLRDMGVEV PDVVAVERIG VAANWQASGG WTDGAHRLGT 70 80 90 100 110 120 SPEKDVGFPY RSALVPRRNA ELDERMTRYS WQSYLIATAS FAEWIDRGRP APTHRRWSQY 130 140 150 160 170 180 LSWVADHVGM TVVHGEVEQL AVTGDRWALH THETTVHADA LMITGPGQAE KSLLPGNPRM 190 200 210 220 230 240 LSIAQFWDRA ANHDRISAER VAVIGGGETA AAMLNELFRH RVSSITVISP QATLFTRGEG 250 260 270 280 290 300 YFENSLFSDP TNWPALTLAE RRDALARTDR GVFSSSVQEA LLADDRIHHL RGRVTHAVGV 310 320 330 340 350 360 QGQIRLTLST NRGSENLETV HGFDLVIDGS GADSLWFAPL FSQEALDLLE LGLGGPLSSE 370 380 390 400 410 420 RLQEAIGYDL AVTDVTPKLF LPNLSGLTQG PGFPNLSCLG LLSDRVLGST LGPTNYPARR RHDERQPL

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016958 Genomic DNA. Translation: AAS04487.1.
RefSeq	NP_961104.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2719638.
GenomeReviews	Gene locus MAP_2170c in contig AE016958_GR.
KEGG	mpa:MAP2170c.
NMPDR	fig|262316.1.peg.2170.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q73XY8.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

MBTG_MYCPA

Accession

Primary (citable) accession number: Q73XY8

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	July 5, 2004
Last modified:	November 4, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)