Reviewed, UniProtKB/Swiss-Prot Q73XM9 (CLPP1_MYCPA)

Names and origin

Protein names

Recommended name:
    ATP-dependent Clp protease proteolytic subunit 1
    EC=3.4.21.92
Alternative name(s):
    Endopeptidase Clp 1

Gene names

Name:	clpP1
Ordered Locus Names:	MAP_2280c

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity HAMAP MF_00444.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	ATP-dependent Clp protease proteolytic subunit 1 HAMAP MF_00444		PRO_0000179594
Sites
	Active site	107	1	By similarity
	Active site	132	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q73XM9-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 791DEBF93F974C19	FASTA	211	23,292
	10 20 30 40 50 60 MNPQIQPQAR YILPSFIEHS SFGIKESNPY NKLFEERIIF LGVQVDDASA NDIMAQLLVL 70 80 90 100 110 120 ESLDPDRDIT MYINSPGGGF TSLMAIYDTM QYVRADIQTV CLGQAASAAA VLLAAGTPGK 130 140 150 160 170 180 RMALPNARVL IHQPSLQGVI QGQFSDLEIQ AAEIERMRTL METTLARHTG KDAATIRKDT 190 200 210 DRDKILTAEE AKDYGIIDTV LEYRKLSAQT A

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS04597.1.
RefSeq	NP_961214.1.
3D structure databases
SMR	Q73XM9. Positions 22-205.
ModBase	Search...
Protein family/group databases
MEROPS	S14.009.
Genome annotation databases
GeneID	2720079.
GenomeReviews	Gene locus MAP_2280c in contig AE016958_GR.
KEGG	mpa:MAP2280c.
NMPDR	fig|262316.1.peg.2280.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG558421.
OMA	RKLSAQK.
Enzyme and pathway databases
BRENDA	3.4.21.92. 189774.
Family and domain databases
HAMAP	MF_00444. ClpP. [Tree]
InterPro	IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP1_MYCPA

Accession

Primary (citable) accession number: Q73XM9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	January 19, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)