Q73X87 (RNPH_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:	rph
Synonyms:	rphA
Ordered Locus Names:	MAP_2422c

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	259 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	tRNA binding Inferred from electronic annotation. Source: InterPro tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: EC tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 259	259	Ribonuclease PH HAMAP MF_00564		PRO_0000139911

Sequences

	Sequence		Length	Mass (Da)
	Q73X87 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: C836BF24308E39F6	FASTA	259	27,375
	10 20 30 40 50 60 MTRREDGRLD DELRPLVITR GFTEHPAGSV LIEFGHTKVM CTASVTEGVP RWRKGSGLGW 70 80 90 100 110 120 LTAEYAMLPS ATHTRSDRES VKGRLSGRTQ EISRLIGRSL RACIDLAALG ENTIAVDCDV 130 140 150 160 170 180 LQADGGTRTA AITGAFVALA DAVTYLSAAG KLSDPRPLSC AIAAVSVGVV DGRIRVDLPY 190 200 210 220 230 240 EEDARAEVDM NVVATDTGTL VEVQGTGEGA TFPRSTLDKL LDAALAACDK LFAAQREALK 250 LPYPGVLPEG PPPPKAFGS

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS04739.1.
RefSeq	NP_961356.1. NC_002944.2.
3D structure databases
ProteinModelPortal	Q73X87.
SMR	Q73X87. Positions 3-248.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000037022; EBMYCP00000035411; EBMYCG00000037017.
GeneID	2717627.
GenomeReviews	Gene locus MAP_2422c in contig AE016958_GR.
KEGG	mpa:MAP2422c.
NMPDR	fig|262316.1.peg.2422.
PATRIC	17997510. VBIMycAvi108102_2573.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000017525.
HOGENOM	HBG737187.
OMA	MLPRATG.
ProtClustDB	PRK00173.
Family and domain databases
HAMAP	MF_00564. RNase_PH. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
KO	K00989.
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_MYCPA

Accession

Primary (citable) accession number: Q73X87

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program