Q73X75 (GLGB_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	MAP_2434

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685		PRO_0000188717
Sites
	Active site	411	1	Nucleophile By similarity
	Active site	464	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q73X75 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: ED7DEAD33683C636	FASTA	731	81,660
	10 20 30 40 50 60 MSHADQLART HLAPDPADLS RLVAGTHHDP HGILGAHEYG DHTVIRAFRP HATEVAALVG 70 80 90 100 110 120 GDRFAMQHIE SGLFAVALPF TNLIDYRLQI TYPDSEPYVV ADAYRFLPTL GEVDLHLFGE 130 140 150 160 170 180 GRHERLWEVL GAHPRSFTTA DGVVTGVSFA VWAPNAKGIS LIGEFNGWTG TEAPMRVLGS 190 200 210 220 230 240 SGVWELFWPD FPIGGLYKFK VHGADGVVTE RADPMAFATE VPPHTASRVT LSSYTWGDAD 250 260 270 280 290 300 WMTQRAARNP VFEPMSTYEV HLGSWRPGLS YRQLARELTD YVVEHGFTHV ELLPVAEHPF 310 320 330 340 350 360 AGSWGYQVTS YYAPTSRFGT PDEFRALVDA LHQAGIGVIV DWVPAHFPKD AWALGRFDGT 370 380 390 400 410 420 PLYEHSDPKR GEQLDWGTYV FDFGRREVRN FLVANALYWL QEFHIDGLRV DAVASMLYLD 430 440 450 460 470 480 YSRPEGGWTP NIYGGRENLE AVQFLQEMNA TAHKSAPGIV TIAEESTSWP GVTRPTNLGG 490 500 510 520 530 540 LGFSMKWNMG WMHDTLDYIS RDPIYRSYHH HEMTFSMLYA FSENYVLPLS HDEVVHGKGT 550 560 570 580 590 600 LWGRMPGNNH VKAAGLRSLL AYQWAHPGKQ LLFMGQEFGQ RAEWSEERGL DWWQLDEQGF 610 620 630 640 650 660 SNGILRLVRD INDIYRSHPA LWSQDTVPDG YSWIDANDSA NNVLSFLRYG KDGSVMACVF 670 680 690 700 710 720 NFAGAEHSGY RLGLPSAGRW REVLNTDATV YNGSGIGNMG GVDATEDPWH GRPASAVLVL 730 PPTSALWLEP K

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS04751.1.
RefSeq	NP_961368.1. NC_002944.2.
3D structure databases
ProteinModelPortal	Q73X75.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000036900; EBMYCP00000035289; EBMYCG00000036895.
GeneID	2720716.
GenomeReviews	Gene locus MAP_2434 in contig AE016958_GR.
KEGG	mpa:MAP2434.
NMPDR	fig|262316.1.peg.2434.
PATRIC	17997534. VBIMycAvi108102_2585.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016657.
HOGENOM	HBG287139.
OMA	DGTCLYE.
ProtClustDB	PRK05402.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_MYCPA

Accession

Primary (citable) accession number: Q73X75

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program