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Q73WX4

- KGD_MYCPA

UniProt

Q73WX4 - KGD_MYCPA

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Protein
Multifunctional 2-oxoglutarate metabolism enzyme
Gene
kgd, MAP_2536
Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Shows three enzymatic activities that share a first common step, the attack of thiamine-PP on 2-oxoglutarate (alpha-ketoglutarate, KG), leading to the formation of an enamine-thiamine-PP intermediate upon decarboxylation. Thus, displays KGD activity, catalyzing the decarboxylation from five-carbon 2-oxoglutarate to four-carbon succinate semialdehyde (SSA). Also catalyzes C-C bond formation between the activated aldehyde formed after decarboxylation of alpha-ketoglutarate and the carbonyl of glyoxylate (GLX), to yield 2-hydroxy-3-oxoadipate (HOA), which spontaneously decarboxylates to form 5-hydroxylevulinate (HLA). And is also a component of the 2-oxoglutarate dehydrogenase (ODH) complex, that catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. The KG decarboxylase and KG dehydrogenase reactions provide two alternative, tightly regulated, pathways connecting the oxidative and reductive branches of the TCA cycle By similarity.

Catalytic activityi

2-oxoglutarate + glyoxylate = 2-hydroxy-3-oxoadipate + CO2.
2-oxoglutarate = succinate semialdehyde + CO2.
2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2.
Succinyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-succinyldihydrolipoyl)lysine.

Cofactori

Magnesium By similarity.
Thiamine pyrophosphate By similarity.

Enzyme regulationi

Alpha-ketoglutarate dehydrogenase and decarboxylase activities are inhibited by unphosphorylated GarA, and allosterically activated by acetyl-CoA, the main substrate of the TCA cycle By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei332 – 3321Proton acceptor; for succinyltransferase activity By similarity
Binding sitei597 – 59712-oxoglutarate By similarity
Binding sitei622 – 62212-oxoglutarate By similarity
Metal bindingi665 – 6651Magnesium By similarity
Metal bindingi698 – 6981Magnesium By similarity
Metal bindingi700 – 7001Magnesium; via carbonyl oxygen By similarity
Binding sitei972 – 9721Thiamine pyrophosphate By similarity
Binding sitei1040 – 104012-oxoglutarate By similarity
Binding sitei1058 – 10581Allosteric activator By similarity
Binding sitei1074 – 10741Allosteric activator By similarity
Binding sitei1162 – 11621Allosteric activator By similarity

GO - Molecular functioni

  1. 2-hydroxy-3-oxoadipate synthase activity Source: UniProtKB-EC
  2. 2-oxoglutarate decarboxylase activity Source: UniProtKB-EC
  3. dihydrolipoyllysine-residue succinyltransferase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW
  5. oxoglutarate dehydrogenase (succinyl-transferring) activity Source: UniProtKB-EC
  6. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Decarboxylase, Lyase, Oxidoreductase, Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciMAVI262316:GCQR-2572-MONOMER.
UniPathwayiUPA00223; UER00997.
UPA00223; UER01001.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional 2-oxoglutarate metabolism enzyme
Alternative name(s):
2-hydroxy-3-oxoadipate synthase (EC:2.2.1.5)
Short name:
HOA synthase
Short name:
HOAS
2-oxoglutarate carboxy-lyase
2-oxoglutarate decarboxylase
Alpha-ketoglutarate decarboxylase (EC:4.1.1.71)
Short name:
KG decarboxylase
Short name:
KGD
Alpha-ketoglutarate-glyoxylate carboligase
Including the following 2 domains:
2-oxoglutarate dehydrogenase E1 component (EC:1.2.4.2)
Short name:
ODH E1 component
Alternative name(s):
Alpha-ketoglutarate dehydrogenase E1 component
Short name:
KDH E1 component
Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (EC:2.3.1.61)
Alternative name(s):
2-oxoglutarate dehydrogenase complex E2 component
Short name:
ODH E2 component
Short name:
OGDC-E2
Dihydrolipoamide succinyltransferase
Gene namesi
Name:kgd
Ordered Locus Names:MAP_2536
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
ProteomesiUP000000580: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12471247Multifunctional 2-oxoglutarate metabolism enzyme
PRO_0000310717Add
BLAST

Interactioni

Subunit structurei

Homodimer. The 2-oxoglutarate dehydrogenase (ODH) complex contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.

Protein-protein interaction databases

STRINGi262316.MAP2536.

Structurei

3D structure databases

ProteinModelPortaliQ73WX4.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 41412-oxoglutarate dehydrogenase E1, N-terminal part
Add
BLAST
Regioni42 – 10463Linker
Add
BLAST
Regioni105 – 353249Succinyltransferase E2
Add
BLAST
Regioni354 – 12478942-oxoglutarate dehydrogenase E1, C-terminal part
Add
BLAST
Regioni557 – 5582Thiamine pyrophosphate binding By similarity
Regioni622 – 6243Thiamine pyrophosphate binding By similarity
Regioni665 – 6673Thiamine pyrophosphate binding By similarity
Regioni1109 – 11124Allosteric activator By similarity
Regioni1169 – 11702Allosteric activator By similarity

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili803 – 83331 Reviewed prediction
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi51 – 12979Ala-rich
Add
BLAST

Domaini

Is a fusion protein with two major domains exhibiting structural features of an E1 and E2 protein, and a short sequence stretch of E1 localized at the N-terminus, which is connected by a linker region to the rest of the protein By similarity.

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0508.
KOiK01616.
OMAiQIDKEAR.
OrthoDBiEOG6V1M1F.

Family and domain databases

Gene3Di3.30.559.10. 1 hit.
3.40.50.970. 2 hits.
InterProiIPR001078. 2-oxoacid_DH_actylTfrase.
IPR011603. 2oxoglutarate_DH_E1.
IPR023213. CAT-like_dom.
IPR001017. DH_E1.
IPR029061. THDP-binding.
IPR005475. Transketolase-like_Pyr-bd.
[Graphical view]
PANTHERiPTHR23152. PTHR23152. 1 hit.
PfamiPF00198. 2-oxoacid_dh. 1 hit.
PF00676. E1_dh. 1 hit.
PF02779. Transket_pyr. 1 hit.
[Graphical view]
PIRSFiPIRSF000157. Oxoglu_dh_E1. 1 hit.
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00239. 2oxo_dh_E1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q73WX4-1 [UniParc]FASTAAdd to Basket

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MSNISSPFGQ NEWLVEEMYR KFRDDPSSVD PSWHEFLVDY NPEPTGDSVL     50
AAPASTDGPS APAPAAPQTA QPALPAQTAP PAQTAQPARP APQPAAAPGN 100
GASTRPAKPA TPPPAEGDEL QTLRGAAAAV VKNMSASLEV PTATSVRAIP 150
AKLLIDNRIV INNQLKRTRG GKISFTHLLG YALVQAIKKF PNMNRHYAEI 200
DGKPTAITPA HTNLGLAIDL QGKDGKRSLV VAGIKNCETM RFAQFVTAYE 250
DIVRRARDGK LTAEDFAGVT ISLTNPGTIG TVHSVPRLMN GQGAIIGVGA 300
MEYPAEFQGA SEERIAELGI GKLITLTSTY DHRIIQGAES GDFLRTIHEM 350
LLSDGFWDDI FRELSIPYLP IRWSTDNPDS IVDKNARVME LIAAYRNRGH 400
LMADIDPLRL DGSRFRSHPD LEILNHGLTL WDLDRVFKVN GFAGAEYKKL 450
RDILGLLRDA YCRHIGVEYT HILDPEQQEW LQQRVETKHV KPTVAEQKFI 500
LSKLNAAEAF ETFLQTKYVG QKRFSLEGAE SVIPMMDAAI DQCAEHGLDE 550
VVIGMPHRGR LNVLANIVGK PYSQIFSEFE GNLNPAQAHG SGDVKYHLGA 600
TGVYLQMFGD NDIQVSLTAN PSHLEAVDPV LEGLVRAKQD LLDHGADDQG 650
DEKAFSVVPM MLHGDAAFAG QGVVAETLNL THLPGYRVGG TIHIIVNNQI 700
GFTTAPEHSR SSEYCTDVAK MIGAPIFHVN GDDPEACAWV AKLAVDFRQK 750
FKKDVVIDML CYRKRGHNEG DDPSMTNPAM YDVVDIKRGV RKSYTEALIG 800
RGDISMKEAE DALRDYQGQL ERVFNEVREL EKHGVGPSES VEADQMLPAG 850
LATAVDKALL ARIGDAFLAL PEGFTAHPRV QPVLEKRREM AYEGKIDWAF 900
AELLALGSLV AGGKLVRLSG QDTRRGTFSQ RHSVIIDRNT GEEFTPLQLL 950
ATNPDGTPTG GKFLVYDSPL SEYAAVGFEY GYTVGNPDAL VLWEAQFGDF 1000
VNGAQSIIDE FISSGEAKWG QLSNVVLLLP HGHEGQGPDH TSARIERFLQ 1050
LWAEGSMTIA MPSTPSNYFH LLRRHALDGI KRPLIVFTPK SMLRNKAAVS 1100
DIKDFTEIKF RSVLEEPTYE DGIGDRGKVS RVLLTSGKLY YELVARKNKD 1150
NRDDVAIVRI EQLAPLPKRR LGETLDRYPN VREYFWVQEE PANQGAWPRF 1200
GLELPELLPD KLTGLKRISR RAMSAPSSGS SKVHAVEQQE IIDTAFA 1247
Length:1,247
Mass (Da):137,073
Last modified:July 5, 2004 - v1
Checksum:iD31E9876D146519A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016958 Genomic DNA. Translation: AAS04853.1.
RefSeqiNP_961470.1. NC_002944.2.

Genome annotation databases

EnsemblBacteriaiAAS04853; AAS04853; MAP_2536.
GeneIDi2719042.
KEGGimpa:MAP2536.
PATRICi17997756. VBIMycAvi108102_2692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE016958 Genomic DNA. Translation: AAS04853.1 .
RefSeqi NP_961470.1. NC_002944.2.

3D structure databases

ProteinModelPortali Q73WX4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 262316.MAP2536.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS04853 ; AAS04853 ; MAP_2536 .
GeneIDi 2719042.
KEGGi mpa:MAP2536.
PATRICi 17997756. VBIMycAvi108102_2692.

Phylogenomic databases

eggNOGi COG0508.
KOi K01616.
OMAi QIDKEAR.
OrthoDBi EOG6V1M1F.

Enzyme and pathway databases

UniPathwayi UPA00223 ; UER00997 .
UPA00223 ; UER01001 .
BioCyci MAVI262316:GCQR-2572-MONOMER.

Family and domain databases

Gene3Di 3.30.559.10. 1 hit.
3.40.50.970. 2 hits.
InterProi IPR001078. 2-oxoacid_DH_actylTfrase.
IPR011603. 2oxoglutarate_DH_E1.
IPR023213. CAT-like_dom.
IPR001017. DH_E1.
IPR029061. THDP-binding.
IPR005475. Transketolase-like_Pyr-bd.
[Graphical view ]
PANTHERi PTHR23152. PTHR23152. 1 hit.
Pfami PF00198. 2-oxoacid_dh. 1 hit.
PF00676. E1_dh. 1 hit.
PF02779. Transket_pyr. 1 hit.
[Graphical view ]
PIRSFi PIRSF000157. Oxoglu_dh_E1. 1 hit.
SMARTi SM00861. Transket_pyr. 1 hit.
[Graphical view ]
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
    Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
    Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-968 / K-10.

Entry informationi

Entry nameiKGD_MYCPA
AccessioniPrimary (citable) accession number: Q73WX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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