Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glucosyl-3-phosphoglycerate synthase

Gene

MAP_2569c

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of methylglucose lipopolysaccharides (MGLPs). Catalyzes the condensation of UDP-glucose and 3-phospho-glycerate (3-PGA) to yield glucosyl-3-phosphoglycerate (GPG). Although UDP-glucose is the natural donor sugar, GpgS can also use UDP-GalNAc, UDPGlcNAc, UDP-alpha-L-arabinose, UDP-Gal, UDP-Glc, UDP-alpha-Dglucuronic acid, and UDP-alpha-D-xylose as donor substrates.1 Publication

Catalytic activityi

NDP-glucose + 3-phospho-D-glycerate = NDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate.1 Publication

Cofactori

Mg2+1 Publication, Ca2+1 Publication, Mn2+1 Publication, Fe2+1 Publication, Co2+1 PublicationNote: Divalent metal cations. Probably Mg2+, but can also use Ca2+, Mn2+, Fe2+ or Co2+ ions.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86NDP-glucose1
Binding sitei116NDP-glucose1
Metal bindingi141Magnesium1
Binding sitei234NDP-glucose1
Sitei263Important for coordination of the donor substrate and for stabilization of the donor substrate product1
Binding sitei266NDP-glucose1
Binding sitei320NDP-glucose1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT81. Glycosyltransferase Family 81.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucosyl-3-phosphoglycerate synthase (EC:2.4.1.266)
Gene namesi
Ordered Locus Names:MAP_2569c
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi
  • UP000000580 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004201641 – 329Glucosyl-3-phosphoglycerate synthaseAdd BLAST329

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi262316.MAP2569c.

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 26Combined sources3
Turni27 – 29Combined sources3
Beta strandi30 – 32Combined sources3
Helixi38 – 43Combined sources6
Turni44 – 47Combined sources4
Beta strandi50 – 58Combined sources9
Turni60 – 62Combined sources3
Helixi63 – 70Combined sources8
Helixi71 – 73Combined sources3
Turni75 – 77Combined sources3
Beta strandi78 – 85Combined sources8
Helixi92 – 98Combined sources7
Beta strandi102 – 105Combined sources4
Helixi106 – 109Combined sources4
Helixi119 – 129Combined sources11
Beta strandi133 – 137Combined sources5
Beta strandi142 – 144Combined sources3
Helixi149 – 158Combined sources10
Beta strandi159 – 162Combined sources4
Beta strandi165 – 171Combined sources7
Helixi190 – 194Combined sources5
Helixi196 – 203Combined sources8
Helixi205 – 209Combined sources5
Beta strandi218 – 221Combined sources4
Helixi222 – 225Combined sources4
Helixi233 – 235Combined sources3
Helixi236 – 248Combined sources13
Helixi250 – 252Combined sources3
Beta strandi253 – 260Combined sources8
Helixi268 – 270Combined sources3
Helixi271 – 285Combined sources15
Beta strandi296 – 302Combined sources7
Beta strandi308 – 312Combined sources5
Helixi323 – 325Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CKJX-ray1.80A1-329[»]
3CKNX-ray2.20A1-329[»]
3CKOX-ray2.50A1-329[»]
3CKQX-ray3.00A1-329[»]
3CKVX-ray2.00A1-329[»]
ProteinModelPortaliQ73WU1.
SMRiQ73WU1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ73WU1.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni55 – 59NDP-glucose binding5
Regioni140 – 141NDP-glucose binding2
Regioni189 – 1923-phospho-D-glycerate bindingBy similarity4

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Phylogenomic databases

eggNOGiENOG4105C3Q. Bacteria.
COG0463. LUCA.
KOiK13693.
OMAiIDPDPMF.
OrthoDBiPOG091H01A8.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q73WU1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTSDLVAGE LAGDGLRDTR PGDTWLADRS WNRPGWTVAE LEAAKAGRTI
60 70 80 90 100
SVVLPALDEE DTIGSVIDSI SPLVDGLVDE LIVLDSGSTD DTEIRAVAAG
110 120 130 140 150
ARVVSREQAL PEVPIRPGKG EALWRSLAAS RGDIVVFVDS DLINPHPMFV
160 170 180 190 200
PWLVGPLLTG DGVHLVKSFY RRPLNVGDAG GGAGATGGGR VTELVARPLL
210 220 230 240 250
AALRPELGCI LQPLGGEYAA TRELLTSVPF APGYGVEIGL LVDTFDRLGL
260 270 280 290 300
DAIAQVNLGV REHRNRPLAE LGAMSRQVIA TLLSRCGIPD SGVGLTQFVA
310 320
DGPEGQSYTQ HTWPVSLADR PPMQAIRPR
Length:329
Mass (Da):34,795
Last modified:July 5, 2004 - v1
Checksum:i06493E07C809B6E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS04886.1.
RefSeqiWP_003878473.1. NC_002944.2.

Genome annotation databases

EnsemblBacteriaiAAS04886; AAS04886; MAP_2569c.
GeneIDi2717539.
KEGGimpa:MAP_2569c.
PATRICi17997830. VBIMycAvi108102_2729.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS04886.1.
RefSeqiWP_003878473.1. NC_002944.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CKJX-ray1.80A1-329[»]
3CKNX-ray2.20A1-329[»]
3CKOX-ray2.50A1-329[»]
3CKQX-ray3.00A1-329[»]
3CKVX-ray2.00A1-329[»]
ProteinModelPortaliQ73WU1.
SMRiQ73WU1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262316.MAP2569c.

Protein family/group databases

CAZyiGT81. Glycosyltransferase Family 81.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS04886; AAS04886; MAP_2569c.
GeneIDi2717539.
KEGGimpa:MAP_2569c.
PATRICi17997830. VBIMycAvi108102_2729.

Phylogenomic databases

eggNOGiENOG4105C3Q. Bacteria.
COG0463. LUCA.
KOiK13693.
OMAiIDPDPMF.
OrthoDBiPOG091H01A8.

Miscellaneous databases

EvolutionaryTraceiQ73WU1.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGPGS_MYCPA
AccessioniPrimary (citable) accession number: Q73WU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2012
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.