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Q73WB6 (CRPE_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Catalase-related peroxidase

EC=1.11.1.-
Gene names
Ordered Locus Names:MAP_2744c
OrganismMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10) [Complete proteome] [HAMAP]
Taxonomic identifier262316 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has an organic peroxide-dependent peroxidase activity. Exhibits strong peroxidase activity using organic hydroperoxides as cosubstrates, weak peroxidase activity using hydrogen peroxide and negligible catalase activity. May have a role in elimination of reactive oxygen species, in particular by deactivating hydroperoxides. Ref.3

Cofactor

Heme group. Ref.2 Ref.3

Subunit structure

Monomer. Ref.3

Sequence similarities

Belongs to the catalase family.

Biophysicochemical properties

Kinetic parameters:

Kcat is 13 sec(-1) for H2O2. Kcat is 320 sec(-1) for t-butyl hydroperoxide. Kcat is 330 sec(-1) for cumene hydroperoxide. Kcat is 529 sec(-1) for 9S-HPODE.

KM=30 mM for H2O2 in 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) oxidation assay (at pH 7.0) Ref.3

KM=22 mM for t-butyl hydroperoxide in ABTS oxidation assay (at pH 7.0)

KM=3 mM for cumene hydroperoxide in ABTS oxidation assay (at pH 7.0)

KM=49 µM for 9S-hydroperoxy-octadecadienoic acid (9S-HPODE) used as a cosubstrate with ABTS (at pH 7.0)

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 313313Catalase-related peroxidase
PRO_0000424152

Sites

Active site281 By similarity
Metal binding2941Iron (heme axial ligand)

Secondary structure

................................................. 313
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q73WB6 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 7EE2BE95125CD14E

FASTA31333,321
        10         20         30         40         50         60 
MSGGLTPDQA IDAIRGTGGA QPGCRALHAK GTLYRGTFTA TRDAVMLSAA PHLDGSTVPA 

        70         80         90        100        110        120 
LIRFSNGSGN PKQRDGAPGV RGMAVKFTLP DGSTTDVSAQ TARLLVSSTP EGFIDLLKAM 

       130        140        150        160        170        180 
RPGLTTPLRL ATHLLTHPRL LGALPLLREA NRIPASYATT EYHGLHAFRW IAADGSARFV 

       190        200        210        220        230        240 
RYHLVPTAAE EYLSASDARG KDPDFLTDEL AARLQDGPVR FDFRVQIAGP TDSTVDPSSA 

       250        260        270        280        290        300 
WQSTQIVTVG TVTITGPDTE REHGGDIVVF DPMRVTDGIE PSDDPVLRFR TLVYSASVKL 

       310 
RTGVDRGAQA PPV 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-968 / K-10.
[2]"Coordination modes of tyrosinate-ligated catalase-type heme enzymes: magnetic circular dichroism studies of Plexaura homomalla allene oxide synthase, Mycobacterium avium ssp. paratuberculosis protein-2744c, and bovine liver catalase in their ferric and ferrous states."
Bandara D.M., Sono M., Bruce G.S., Brash A.R., Dawson J.H.
J. Inorg. Biochem. 105:1786-1794(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: MAGNETIC CIRCULAR DICHROISM, COFACTOR.
[3]"The structure and peroxidase activity of a 33-kDa catalase-related protein from Mycobacterium avium ssp. paratuberculosis."
Pakhomova S., Gao B., Boeglin W.E., Brash A.R., Newcomer M.E.
Protein Sci. 18:2559-2568(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH HEME, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016958 Genomic DNA. Translation: AAS05061.1.
RefSeqNP_961678.1. NC_002944.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3E4WX-ray1.80A/B1-313[»]
3E4YX-ray2.60A1-313[»]
ProteinModelPortalQ73WB6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING262316.MAP2744c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS05061; AAS05061; MAP_2744c.
GeneID2720786.
KEGGmpa:MAP2744c.
PATRIC17998202. VBIMycAvi108102_2915.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK03781.
OMAANAKPHG.
OrthoDBEOG6742T3.
ProtClustDBCLSK763620.

Enzyme and pathway databases

BioCycMAVI262316:GCQR-2780-MONOMER.

Family and domain databases

Gene3D2.40.180.10. 1 hit.
InterProIPR018028. Catalase.
IPR020835. Catalase-like_dom.
IPR011614. Catalase_core.
IPR024168. Catalase_SrpA-type_pred.
[Graphical view]
PANTHERPTHR11465. PTHR11465. 1 hit.
PfamPF00199. Catalase. 1 hit.
[Graphical view]
PIRSFPIRSF000296. SrpA. 1 hit.
PRINTSPR00067. CATALASE.
SMARTSM01060. Catalase. 1 hit.
[Graphical view]
SUPFAMSSF56634. SSF56634. 1 hit.
PROSITEPS51402. CATALASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ73WB6.

Entry information

Entry nameCRPE_MYCPA
AccessionPrimary (citable) accession number: Q73WB6
Entry history
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references