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Q73WB6

- CRPE_MYCPA

UniProt

Q73WB6 - CRPE_MYCPA

Protein

Catalase-related peroxidase

Gene

MAP_2744c

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Has an organic peroxide-dependent peroxidase activity. Exhibits strong peroxidase activity using organic hydroperoxides as cosubstrates, weak peroxidase activity using hydrogen peroxide and negligible catalase activity. May have a role in elimination of reactive oxygen species, in particular by deactivating hydroperoxides.1 Publication

    Cofactori

    Heme group.2 Publications

    Kineticsi

    Kcat is 13 sec(-1) for H2O2. Kcat is 320 sec(-1) for t-butyl hydroperoxide. Kcat is 330 sec(-1) for cumene hydroperoxide. Kcat is 529 sec(-1) for 9S-HPODE.1 Publication

    1. KM=30 mM for H2O2 in 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) oxidation assay (at pH 7.0)1 Publication
    2. KM=22 mM for t-butyl hydroperoxide in ABTS oxidation assay (at pH 7.0)1 Publication
    3. KM=3 mM for cumene hydroperoxide in ABTS oxidation assay (at pH 7.0)1 Publication
    4. KM=49 µM for 9S-hydroperoxy-octadecadienoic acid (9S-HPODE) used as a cosubstrate with ABTS (at pH 7.0)1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei28 – 281By similarity
    Metal bindingi294 – 2941Iron (heme axial ligand)

    GO - Molecular functioni

    1. catalase activity Source: InterPro
    2. heme binding Source: UniProtKB
    3. iron ion binding Source: UniProtKB
    4. peroxidase activity Source: UniProtKB

    GO - Biological processi

    1. hydrogen peroxide metabolic process Source: UniProtKB
    2. oxidation-reduction process Source: UniProtKB
    3. regulation of reactive oxygen species metabolic process Source: UniProtKB
    4. response to oxidative stress Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase, Peroxidase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciMAVI262316:GCQR-2780-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Catalase-related peroxidase1 Publication (EC:1.11.1.-1 Publication)
    Gene namesi
    Ordered Locus Names:MAP_2744c
    OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
    Taxonomic identifieri262316 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
    ProteomesiUP000000580: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Catalase-related peroxidasePRO_0000424152Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi262316.MAP2744c.

    Structurei

    Secondary structure

    1
    313
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 1610
    Beta strandi26 – 283
    Beta strandi30 – 4011
    Helixi44 – 463
    Helixi51 – 533
    Beta strandi57 – 6913
    Beta strandi81 – 888
    Beta strandi94 – 1018
    Helixi110 – 11910
    Beta strandi121 – 1233
    Helixi126 – 13611
    Helixi138 – 1414
    Helixi144 – 1507
    Beta strandi153 – 1553
    Helixi157 – 1593
    Beta strandi162 – 17110
    Beta strandi177 – 18812
    Helixi195 – 1995
    Helixi205 – 21612
    Beta strandi219 – 2279
    Beta strandi246 – 25712
    Beta strandi260 – 2634
    Beta strandi279 – 2813
    Helixi285 – 30218

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3E4WX-ray1.80A/B1-313[»]
    3E4YX-ray2.60A1-313[»]
    ProteinModelPortaliQ73WB6.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ73WB6.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the catalase family.Sequence Analysis

    Phylogenomic databases

    KOiK03781.
    OMAiGNDYLED.
    OrthoDBiEOG6742T3.

    Family and domain databases

    Gene3Di2.40.180.10. 1 hit.
    InterProiIPR018028. Catalase.
    IPR020835. Catalase-like_dom.
    IPR011614. Catalase_core.
    IPR024168. Catalase_SrpA-type_pred.
    [Graphical view]
    PANTHERiPTHR11465. PTHR11465. 1 hit.
    PfamiPF00199. Catalase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000296. SrpA. 1 hit.
    PRINTSiPR00067. CATALASE.
    SMARTiSM01060. Catalase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56634. SSF56634. 1 hit.
    PROSITEiPS51402. CATALASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q73WB6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGGLTPDQA IDAIRGTGGA QPGCRALHAK GTLYRGTFTA TRDAVMLSAA    50
    PHLDGSTVPA LIRFSNGSGN PKQRDGAPGV RGMAVKFTLP DGSTTDVSAQ 100
    TARLLVSSTP EGFIDLLKAM RPGLTTPLRL ATHLLTHPRL LGALPLLREA 150
    NRIPASYATT EYHGLHAFRW IAADGSARFV RYHLVPTAAE EYLSASDARG 200
    KDPDFLTDEL AARLQDGPVR FDFRVQIAGP TDSTVDPSSA WQSTQIVTVG 250
    TVTITGPDTE REHGGDIVVF DPMRVTDGIE PSDDPVLRFR TLVYSASVKL 300
    RTGVDRGAQA PPV 313
    Length:313
    Mass (Da):33,321
    Last modified:July 5, 2004 - v1
    Checksum:i7EE2BE95125CD14E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016958 Genomic DNA. Translation: AAS05061.1.
    RefSeqiNP_961678.1. NC_002944.2.

    Genome annotation databases

    EnsemblBacteriaiAAS05061; AAS05061; MAP_2744c.
    GeneIDi2720786.
    KEGGimpa:MAP2744c.
    PATRICi17998202. VBIMycAvi108102_2915.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE016958 Genomic DNA. Translation: AAS05061.1 .
    RefSeqi NP_961678.1. NC_002944.2.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3E4W X-ray 1.80 A/B 1-313 [» ]
    3E4Y X-ray 2.60 A 1-313 [» ]
    ProteinModelPortali Q73WB6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 262316.MAP2744c.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAS05061 ; AAS05061 ; MAP_2744c .
    GeneIDi 2720786.
    KEGGi mpa:MAP2744c.
    PATRICi 17998202. VBIMycAvi108102_2915.

    Phylogenomic databases

    KOi K03781.
    OMAi GNDYLED.
    OrthoDBi EOG6742T3.

    Enzyme and pathway databases

    BioCyci MAVI262316:GCQR-2780-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei Q73WB6.

    Family and domain databases

    Gene3Di 2.40.180.10. 1 hit.
    InterProi IPR018028. Catalase.
    IPR020835. Catalase-like_dom.
    IPR011614. Catalase_core.
    IPR024168. Catalase_SrpA-type_pred.
    [Graphical view ]
    PANTHERi PTHR11465. PTHR11465. 1 hit.
    Pfami PF00199. Catalase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000296. SrpA. 1 hit.
    PRINTSi PR00067. CATALASE.
    SMARTi SM01060. Catalase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56634. SSF56634. 1 hit.
    PROSITEi PS51402. CATALASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
      Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
      Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-968 / K-10.
    2. "Coordination modes of tyrosinate-ligated catalase-type heme enzymes: magnetic circular dichroism studies of Plexaura homomalla allene oxide synthase, Mycobacterium avium ssp. paratuberculosis protein-2744c, and bovine liver catalase in their ferric and ferrous states."
      Bandara D.M., Sono M., Bruce G.S., Brash A.R., Dawson J.H.
      J. Inorg. Biochem. 105:1786-1794(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: MAGNETIC CIRCULAR DICHROISM, COFACTOR.
    3. "The structure and peroxidase activity of a 33-kDa catalase-related protein from Mycobacterium avium ssp. paratuberculosis."
      Pakhomova S., Gao B., Boeglin W.E., Brash A.R., Newcomer M.E.
      Protein Sci. 18:2559-2568(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH HEME, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

    Entry informationi

    Entry nameiCRPE_MYCPA
    AccessioniPrimary (citable) accession number: Q73WB6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 16, 2013
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3