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Protein

Catalase-related peroxidase

Gene

MAP_2744c

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has an organic peroxide-dependent peroxidase activity. Exhibits strong peroxidase activity using organic hydroperoxides as cosubstrates, weak peroxidase activity using hydrogen peroxide and negligible catalase activity. May have a role in elimination of reactive oxygen species, in particular by deactivating hydroperoxides.1 Publication

Cofactori

heme2 Publications

Kineticsi

Kcat is 13 sec(-1) for H2O2. Kcat is 320 sec(-1) for t-butyl hydroperoxide. Kcat is 330 sec(-1) for cumene hydroperoxide. Kcat is 529 sec(-1) for 9S-HPODE.1 Publication

Manual assertion based on experiment ini

  1. KM=30 mM for H2O2 in 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) oxidation assay (at pH 7.0)1 Publication
  2. KM=22 mM for t-butyl hydroperoxide in ABTS oxidation assay (at pH 7.0)1 Publication
  3. KM=3 mM for cumene hydroperoxide in ABTS oxidation assay (at pH 7.0)1 Publication
  4. KM=49 µM for 9S-hydroperoxy-octadecadienoic acid (9S-HPODE) used as a cosubstrate with ABTS (at pH 7.0)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei28By similarity1
    Metal bindingi294Iron (heme axial ligand)1

    GO - Molecular functioni

    • catalase activity Source: InterPro
    • heme binding Source: UniProtKB
    • iron ion binding Source: UniProtKB
    • peroxidase activity Source: UniProtKB

    GO - Biological processi

    • hydrogen peroxide metabolic process Source: UniProtKB
    • oxidation-reduction process Source: UniProtKB
    • regulation of reactive oxygen species metabolic process Source: UniProtKB
    • response to oxidative stress Source: InterPro
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase, Peroxidase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Catalase-related peroxidase1 Publication (EC:1.11.1.-1 Publication)
    Gene namesi
    Ordered Locus Names:MAP_2744c
    OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
    Taxonomic identifieri262316 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
    Proteomesi
    • UP000000580 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004241521 – 313Catalase-related peroxidaseAdd BLAST313

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi262316.MAP2744c.

    Structurei

    Secondary structure

    1313
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi7 – 16Combined sources10
    Beta strandi26 – 28Combined sources3
    Beta strandi30 – 40Combined sources11
    Helixi44 – 46Combined sources3
    Helixi51 – 53Combined sources3
    Beta strandi57 – 69Combined sources13
    Beta strandi81 – 88Combined sources8
    Beta strandi94 – 101Combined sources8
    Helixi110 – 119Combined sources10
    Beta strandi121 – 123Combined sources3
    Helixi126 – 136Combined sources11
    Helixi138 – 141Combined sources4
    Helixi144 – 150Combined sources7
    Beta strandi153 – 155Combined sources3
    Helixi157 – 159Combined sources3
    Beta strandi162 – 171Combined sources10
    Beta strandi177 – 188Combined sources12
    Helixi195 – 199Combined sources5
    Helixi205 – 216Combined sources12
    Beta strandi219 – 227Combined sources9
    Beta strandi246 – 257Combined sources12
    Beta strandi260 – 263Combined sources4
    Beta strandi279 – 281Combined sources3
    Helixi285 – 302Combined sources18

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3E4WX-ray1.80A/B1-313[»]
    3E4YX-ray2.60A1-313[»]
    ProteinModelPortaliQ73WB6.
    SMRiQ73WB6.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ73WB6.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the catalase family.Sequence analysis

    Phylogenomic databases

    eggNOGiENOG4107VGR. Bacteria.
    COG0753. LUCA.
    KOiK03781.
    OMAiIFPMRSA.
    OrthoDBiPOG091H08GE.

    Family and domain databases

    CDDicd08153. srpA_like. 1 hit.
    Gene3Di2.40.180.10. 1 hit.
    InterProiIPR018028. Catalase.
    IPR020835. Catalase-like_dom.
    IPR011614. Catalase_core.
    IPR024168. Catalase_SrpA-type_pred.
    [Graphical view]
    PANTHERiPTHR11465. PTHR11465. 1 hit.
    PfamiPF00199. Catalase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000296. SrpA. 1 hit.
    PRINTSiPR00067. CATALASE.
    SMARTiSM01060. Catalase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56634. SSF56634. 1 hit.
    PROSITEiPS51402. CATALASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q73WB6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSGGLTPDQA IDAIRGTGGA QPGCRALHAK GTLYRGTFTA TRDAVMLSAA
    60 70 80 90 100
    PHLDGSTVPA LIRFSNGSGN PKQRDGAPGV RGMAVKFTLP DGSTTDVSAQ
    110 120 130 140 150
    TARLLVSSTP EGFIDLLKAM RPGLTTPLRL ATHLLTHPRL LGALPLLREA
    160 170 180 190 200
    NRIPASYATT EYHGLHAFRW IAADGSARFV RYHLVPTAAE EYLSASDARG
    210 220 230 240 250
    KDPDFLTDEL AARLQDGPVR FDFRVQIAGP TDSTVDPSSA WQSTQIVTVG
    260 270 280 290 300
    TVTITGPDTE REHGGDIVVF DPMRVTDGIE PSDDPVLRFR TLVYSASVKL
    310
    RTGVDRGAQA PPV
    Length:313
    Mass (Da):33,321
    Last modified:July 5, 2004 - v1
    Checksum:i7EE2BE95125CD14E
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE016958 Genomic DNA. Translation: AAS05061.1.
    RefSeqiWP_003875322.1. NC_002944.2.

    Genome annotation databases

    EnsemblBacteriaiAAS05061; AAS05061; MAP_2744c.
    GeneIDi2720786.
    KEGGimpa:MAP_2744c.
    PATRICi17998202. VBIMycAvi108102_2915.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE016958 Genomic DNA. Translation: AAS05061.1.
    RefSeqiWP_003875322.1. NC_002944.2.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    3E4WX-ray1.80A/B1-313[»]
    3E4YX-ray2.60A1-313[»]
    ProteinModelPortaliQ73WB6.
    SMRiQ73WB6.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi262316.MAP2744c.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAS05061; AAS05061; MAP_2744c.
    GeneIDi2720786.
    KEGGimpa:MAP_2744c.
    PATRICi17998202. VBIMycAvi108102_2915.

    Phylogenomic databases

    eggNOGiENOG4107VGR. Bacteria.
    COG0753. LUCA.
    KOiK03781.
    OMAiIFPMRSA.
    OrthoDBiPOG091H08GE.

    Miscellaneous databases

    EvolutionaryTraceiQ73WB6.

    Family and domain databases

    CDDicd08153. srpA_like. 1 hit.
    Gene3Di2.40.180.10. 1 hit.
    InterProiIPR018028. Catalase.
    IPR020835. Catalase-like_dom.
    IPR011614. Catalase_core.
    IPR024168. Catalase_SrpA-type_pred.
    [Graphical view]
    PANTHERiPTHR11465. PTHR11465. 1 hit.
    PfamiPF00199. Catalase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000296. SrpA. 1 hit.
    PRINTSiPR00067. CATALASE.
    SMARTiSM01060. Catalase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56634. SSF56634. 1 hit.
    PROSITEiPS51402. CATALASE_3. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCRPE_MYCPA
    AccessioniPrimary (citable) accession number: Q73WB6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 16, 2013
    Last sequence update: July 5, 2004
    Last modified: November 2, 2016
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.