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Q73WB6

- CRPE_MYCPA

UniProt

Q73WB6 - CRPE_MYCPA

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Protein

Catalase-related peroxidase

Gene

MAP_2744c

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Has an organic peroxide-dependent peroxidase activity. Exhibits strong peroxidase activity using organic hydroperoxides as cosubstrates, weak peroxidase activity using hydrogen peroxide and negligible catalase activity. May have a role in elimination of reactive oxygen species, in particular by deactivating hydroperoxides.1 Publication

Cofactori

heme2 Publications

Kineticsi

Kcat is 13 sec(-1) for H2O2. Kcat is 320 sec(-1) for t-butyl hydroperoxide. Kcat is 330 sec(-1) for cumene hydroperoxide. Kcat is 529 sec(-1) for 9S-HPODE.1 Publication

  1. KM=30 mM for H2O2 in 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) oxidation assay (at pH 7.0)1 Publication
  2. KM=22 mM for t-butyl hydroperoxide in ABTS oxidation assay (at pH 7.0)1 Publication
  3. KM=3 mM for cumene hydroperoxide in ABTS oxidation assay (at pH 7.0)1 Publication
  4. KM=49 µM for 9S-hydroperoxy-octadecadienoic acid (9S-HPODE) used as a cosubstrate with ABTS (at pH 7.0)1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei28 – 281By similarity
Metal bindingi294 – 2941Iron (heme axial ligand)

GO - Molecular functioni

  1. catalase activity Source: InterPro
  2. heme binding Source: UniProtKB
  3. iron ion binding Source: UniProtKB
  4. peroxidase activity Source: UniProtKB

GO - Biological processi

  1. hydrogen peroxide metabolic process Source: UniProtKB
  2. oxidation-reduction process Source: UniProtKB
  3. regulation of reactive oxygen species metabolic process Source: UniProtKB
  4. response to oxidative stress Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Peroxidase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciMAVI262316:GCQR-2780-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Catalase-related peroxidase1 Publication (EC:1.11.1.-1 Publication)
Gene namesi
Ordered Locus Names:MAP_2744c
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
ProteomesiUP000000580: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 313313Catalase-related peroxidasePRO_0000424152Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi262316.MAP2744c.

Structurei

Secondary structure

1
313
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 1610Combined sources
Beta strandi26 – 283Combined sources
Beta strandi30 – 4011Combined sources
Helixi44 – 463Combined sources
Helixi51 – 533Combined sources
Beta strandi57 – 6913Combined sources
Beta strandi81 – 888Combined sources
Beta strandi94 – 1018Combined sources
Helixi110 – 11910Combined sources
Beta strandi121 – 1233Combined sources
Helixi126 – 13611Combined sources
Helixi138 – 1414Combined sources
Helixi144 – 1507Combined sources
Beta strandi153 – 1553Combined sources
Helixi157 – 1593Combined sources
Beta strandi162 – 17110Combined sources
Beta strandi177 – 18812Combined sources
Helixi195 – 1995Combined sources
Helixi205 – 21612Combined sources
Beta strandi219 – 2279Combined sources
Beta strandi246 – 25712Combined sources
Beta strandi260 – 2634Combined sources
Beta strandi279 – 2813Combined sources
Helixi285 – 30218Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3E4WX-ray1.80A/B1-313[»]
3E4YX-ray2.60A1-313[»]
ProteinModelPortaliQ73WB6.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ73WB6.

Family & Domainsi

Sequence similaritiesi

Belongs to the catalase family.Sequence Analysis

Phylogenomic databases

KOiK03781.
OMAiGNDYLED.
OrthoDBiEOG6742T3.

Family and domain databases

Gene3Di2.40.180.10. 1 hit.
InterProiIPR018028. Catalase.
IPR020835. Catalase-like_dom.
IPR011614. Catalase_core.
IPR024168. Catalase_SrpA-type_pred.
[Graphical view]
PANTHERiPTHR11465. PTHR11465. 1 hit.
PfamiPF00199. Catalase. 1 hit.
[Graphical view]
PIRSFiPIRSF000296. SrpA. 1 hit.
PRINTSiPR00067. CATALASE.
SMARTiSM01060. Catalase. 1 hit.
[Graphical view]
SUPFAMiSSF56634. SSF56634. 1 hit.
PROSITEiPS51402. CATALASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q73WB6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGGLTPDQA IDAIRGTGGA QPGCRALHAK GTLYRGTFTA TRDAVMLSAA
60 70 80 90 100
PHLDGSTVPA LIRFSNGSGN PKQRDGAPGV RGMAVKFTLP DGSTTDVSAQ
110 120 130 140 150
TARLLVSSTP EGFIDLLKAM RPGLTTPLRL ATHLLTHPRL LGALPLLREA
160 170 180 190 200
NRIPASYATT EYHGLHAFRW IAADGSARFV RYHLVPTAAE EYLSASDARG
210 220 230 240 250
KDPDFLTDEL AARLQDGPVR FDFRVQIAGP TDSTVDPSSA WQSTQIVTVG
260 270 280 290 300
TVTITGPDTE REHGGDIVVF DPMRVTDGIE PSDDPVLRFR TLVYSASVKL
310
RTGVDRGAQA PPV
Length:313
Mass (Da):33,321
Last modified:July 5, 2004 - v1
Checksum:i7EE2BE95125CD14E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS05061.1.
RefSeqiNP_961678.1. NC_002944.2.

Genome annotation databases

EnsemblBacteriaiAAS05061; AAS05061; MAP_2744c.
GeneIDi2720786.
KEGGimpa:MAP2744c.
PATRICi17998202. VBIMycAvi108102_2915.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS05061.1 .
RefSeqi NP_961678.1. NC_002944.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3E4W X-ray 1.80 A/B 1-313 [» ]
3E4Y X-ray 2.60 A 1-313 [» ]
ProteinModelPortali Q73WB6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 262316.MAP2744c.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS05061 ; AAS05061 ; MAP_2744c .
GeneIDi 2720786.
KEGGi mpa:MAP2744c.
PATRICi 17998202. VBIMycAvi108102_2915.

Phylogenomic databases

KOi K03781.
OMAi GNDYLED.
OrthoDBi EOG6742T3.

Enzyme and pathway databases

BioCyci MAVI262316:GCQR-2780-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q73WB6.

Family and domain databases

Gene3Di 2.40.180.10. 1 hit.
InterProi IPR018028. Catalase.
IPR020835. Catalase-like_dom.
IPR011614. Catalase_core.
IPR024168. Catalase_SrpA-type_pred.
[Graphical view ]
PANTHERi PTHR11465. PTHR11465. 1 hit.
Pfami PF00199. Catalase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000296. SrpA. 1 hit.
PRINTSi PR00067. CATALASE.
SMARTi SM01060. Catalase. 1 hit.
[Graphical view ]
SUPFAMi SSF56634. SSF56634. 1 hit.
PROSITEi PS51402. CATALASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
    Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
    Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-968 / K-10.
  2. "Coordination modes of tyrosinate-ligated catalase-type heme enzymes: magnetic circular dichroism studies of Plexaura homomalla allene oxide synthase, Mycobacterium avium ssp. paratuberculosis protein-2744c, and bovine liver catalase in their ferric and ferrous states."
    Bandara D.M., Sono M., Bruce G.S., Brash A.R., Dawson J.H.
    J. Inorg. Biochem. 105:1786-1794(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: MAGNETIC CIRCULAR DICHROISM, COFACTOR.
  3. "The structure and peroxidase activity of a 33-kDa catalase-related protein from Mycobacterium avium ssp. paratuberculosis."
    Pakhomova S., Gao B., Boeglin W.E., Brash A.R., Newcomer M.E.
    Protein Sci. 18:2559-2568(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS) IN COMPLEX WITH HEME, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Entry informationi

Entry nameiCRPE_MYCPA
AccessioniPrimary (citable) accession number: Q73WB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3