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Protein

Thymidylate synthase

Gene

thyA

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei24dUMPUniRule annotation1
Binding sitei545,10-methylenetetrahydrofolateUniRule annotation1
Active sitei149NucleophileUniRule annotation1
Binding sitei1725,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei180dUMPUniRule annotation1
Binding sitei2655,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi129 – 130dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi169 – 172dUMPUniRule annotation4
Nucleotide bindingi210 – 212dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

BioCyciMAVI262316:G1G0E-2996-MONOMER
UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:MAP_2869c
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001409851 – 266Thymidylate synthaseAdd BLAST266

Proteomic databases

PRIDEiQ73VZ2

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi262316.MAP2869c

Structurei

3D structure databases

ProteinModelPortaliQ73VZ2
SMRiQ73VZ2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0207 LUCA
KOiK00560
OMAiKQYLDLC
OrthoDBiPOG091H02L6

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
TIGRFAMsiTIGR03284 thym_sym, 2 hits
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q73VZ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPIPTPYEDL LRLVLDRGTA KSDRTGTGTR SLFGQQLRYD LSAGFPLITT
60 70 80 90 100
KKVHLKSVVY ELLWFLRGDS NVDWLHRHGV TIWDEWASDT GDLGPIYGVQ
110 120 130 140 150
WRSWPTPSGE HIDQISAALD LLRTDPDSRR IIVSAWNVGE IPRMALPPCH
160 170 180 190 200
AFFQFYVADG RLSCQLYQRS ADLFLGVPFN IASYALLTHM MAAQAGLSVG
210 220 230 240 250
EFVWTGGDCH IYDNHVEQVR LQLSREPRPY PELILAHRDS IFDYTYDDVV
260
VHNYDPHPAI KAPVAV
Length:266
Mass (Da):30,148
Last modified:July 5, 2004 - v1
Checksum:iE0A68592B333FE8C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA Translation: AAS05186.1
RefSeqiWP_003875188.1, NC_002944.2

Genome annotation databases

EnsemblBacteriaiAAS05186; AAS05186; MAP_2869c
GeneIDi2718423
KEGGimpa:MAP_2869c
PATRICifig|262316.17.peg.3039

Entry informationi

Entry nameiTYSY_MYCPA
AccessioniPrimary (citable) accession number: Q73VZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 5, 2004
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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