Q73VP3 (RNH2_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	MAP_2970c

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	Ribonuclease HII HAMAP MF_00052_B		PRO_0000235737
Sites
	Metal binding	36	1	Divalent metal cation By similarity
	Metal binding	37	1	Divalent metal cation By similarity
	Metal binding	130	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q73VP3 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 29158A50DABBC9AB	FASTA	239	25,227
	10 20 30 40 50 60 MATTWPPRTV IRKSSGLRTL ESALYRSGLG PVAGVDEVGR GACAGPLVVA ACVLGPGKLE 70 80 90 100 110 120 SLAALDDSKK LTESVRERLF PVIRRYALAY HVVFIPPAEV DRRGVHVANI EGMRRAVAGL 130 140 150 160 170 180 SLRPGYVLSD GFRVPGLAVP SLPVIGGDAV AACIAAASVL AKVSRDRLMV AMDAEHPGYG 190 200 210 220 230 FADHKGYSTP AHSAALARLG PCPQHRHSFI NVRRVANGSG GRVVADCKPD LPLQRDEGR

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS05287.1.
RefSeq	NP_961904.1. NC_002944.2.
3D structure databases
ProteinModelPortal	Q73VP3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000037388; EBMYCP00000035777; EBMYCG00000037383.
GeneID	2718023.
GenomeReviews	Gene locus MAP_2970c in contig AE016958_GR.
KEGG	mpa:MAP2970c.
NMPDR	fig|262316.1.peg.2970.
PATRIC	17998674. VBIMycAvi108102_3147.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016477.
HOGENOM	HBG584843.
OMA	RLGPTPI.
ProtClustDB	PRK00015.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_MYCPA

Accession

Primary (citable) accession number: Q73VP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program