Q73VN9 (TRMD_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase
EC=2.1.1.228

Alternative name(s):
M1G-methyltransferase
tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	MAP_2974c

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	239 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605
Subunit structure	Homodimer By similarity. HAMAP MF_00605
Subcellular location	Cytoplasm Potential HAMAP MF_00605.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 239	239	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060409
Regions
	Region	133 – 138	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	109	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q73VN9 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 41A22665C37ED6E7	FASTA	239	26,326
	10 20 30 40 50 60 MRIDVVTIFP AYLDPLRQSL PGKAIQSGLV DLRVHDLRRW THDAHRSVDD APYGGGPGMV 70 80 90 100 110 120 MKAPVWGEAL DEIASAETLL VVPTPAGVLF DQATAARWSA ERHLVFACGR YEGIDQRVVE 130 140 150 160 170 180 DAARRMRVEE VSIGDYVLPG GESAAVVMIE AVLRLLDGVL GNPASRHDDS HSPALDRRLE 190 200 210 220 230 GPSYTRPPSW RGLDVPEVLL SGDHARIAAW RREVSLQRTR ERRPELLADP VGPQDDPGR

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS05291.1.
RefSeq	NP_961908.1. NC_002944.2.
3D structure databases
ProteinModelPortal	Q73VN9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000039602; EBMYCP00000037991; EBMYCG00000039597.
GeneID	2719466.
GenomeReviews	Gene locus MAP_2974c in contig AE016958_GR.
KEGG	mpa:MAP2974c.
NMPDR	fig|262316.1.peg.2974.
PATRIC	17998682. VBIMycAvi108102_3151.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016420.
HOGENOM	HBG285805.
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_MYCPA

Accession

Primary (citable) accession number: Q73VN9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program