Q73VI4 (SYE_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	MAP_3029c

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	492 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 492	492	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000119606
Regions
	Motif	13 – 23	11	"HIGH" region HAMAP MF_00022_B
	Motif	257 – 261	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	260	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q73VI4 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4FFA432D41A83936	FASTA	492	54,019
	10 20 30 40 50 60 MTAPANVRVR FCPSPTGTPH VGMVRTALFN WAYARHTGGT FVFRIEDTDA ARDSEESYLA 70 80 90 100 110 120 LLDALRWLGL DWDEGPEVGG PYGPYRQSQR GEIYRDVVAK LVEAGEAYYA FSTPEEVEAR 130 140 150 160 170 180 HIAAGRNPKL GYDNFDRHLT DSQRAAFLAE GRQPVVRLRM PDEDLGWDDL VRGSTVFAAG 190 200 210 220 230 240 SVPDFALTRA NGDPLYTLVN PCDDALMKIT HVLRGEDLLP STPRQLALYQ ALIRIGVAER 250 260 270 280 290 300 IPQFAHLPTV LGEGTKKLSK RDPQSNLFAH RDRGFIPEGL LNYLALLGWA IADDRDVFSL 310 320 330 340 350 360 DEMVAAFDVV DVNSNPARFD QKKADALNAE HIRMLTAADF AARLRHYLDV HGHRLALDDA 370 380 390 400 410 420 AFATAAELVQ TRIVVLGDAW DLLKFLNDDE YAIDPKAAAK ELGPDGAAVL DAALPALDAV 430 440 450 460 470 480 TDWAAPQIEA ALKTALIDGL GLKPRKAFGP IRVGATGTTI SPPLFESLQL LGRDRSLRRL 490 RAARERAGQH SA

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS05577.1.
RefSeq	NP_961963.1. NC_002944.2.
3D structure databases
ProteinModelPortal	Q73VI4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000038150; EBMYCP00000036539; EBMYCG00000038145.
GeneID	2718032.
GenomeReviews	Gene locus MAP_3029c in contig AE016958_GR.
KEGG	mpa:MAP3029c.
NMPDR	fig|262316.1.peg.3029.
PATRIC	17998802. VBIMycAvi108102_3209.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016746.
HOGENOM	HBG628189.
OMA	MAHIPLI.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_MYCPA

Accession

Primary (citable) accession number: Q73VI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program