Reviewed, UniProtKB/Swiss-Prot Q73U92 (DNAE2_MYCPA)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	MAP_3476c

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	1093 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1093	1093	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103384

Sequences

	Sequence		Length	Mass (Da)
	Q73U92-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 9AB2F22EFD7BFF35	FASTA	1,093	118,223
	10 20 30 40 50 60 MGWFNGPPSW AEMERVLDSK PRRAGESAAP EPDGPLSRGR ATYRPPDEGR AARSSVPYAE 70 80 90 100 110 120 LHAHSAFSFL DGASTPEEMV EEAARLDLRA LALTDHDGLY GAVRFAEAAA ELDVRTVFGA 130 140 150 160 170 180 ELSLGPSART EAPDPPGPHL LVLARGPEGY RRLSRQLAAA HLAGGEKGKP RYDLDALTEA 190 200 210 220 230 240 AGGHWHILTG CRKGHVRQAL SDGGPDAAAR ALADLVDRFG AARVSIELTR HGQPLDDERN 250 260 270 280 290 300 AALAALAPRF GVGVVATTGA HFAGPSRRRL AMAMGAIRAR ESLDSAAGWL APLGGSHLRS 310 320 330 340 350 360 GAEMARLFAW RPQAVTAAAE LGEQCAFGLA LIAPRLPPFD VPDGHTEDSW LRQLTMTGAR 370 380 390 400 410 420 DRYGSPEHAP RAYAQIEHEL KVIAQLQFPG YFLVVHDIAR FCRENNILCQ GRGSAANSAV 430 440 450 460 470 480 CYALGVTAVD PVANELLFER FLSPARDGPP DIDMDIESDQ REKVIQYVYD RYGRDYAAQV 490 500 510 520 530 540 ANVITYRGKI AVRDMARALG YSQGQQDAWS KQISSWSGPA DSPDVEGIPP QVIDLANQVR 550 560 570 580 590 600 NLPRHLGIHS GGMVICDRPI ADVCPVEWAR MENRSVLQWD KDDCAAIGLV KFDLLGLGML 610 620 630 640 650 660 SALHYAIDLV AEHKGIEVDL ARLDLSEPAV YEMLARADSV GVFQVESRAQ MATLPRLKPR 670 680 690 700 710 720 VFYDLVVEVA LIRPGPIQGG SVHPYIRRRN GVDPVLYDHP SMEPALRKTL GVPLFQEQLM 730 740 750 760 770 780 QLAVDCAGFS AAEADQLRRA MGSKRSTERM RRLRSRFYDG MRALHGAPDE VIDRTYEKLE 790 800 810 820 830 840 AFANFGFPES HALSFASLVF YSSWFKLHHP AAFCAALLRA QPMGFYSPQS LVADARRHGV 850 860 870 880 890 900 TVHGPDVNAS LAHATLENAG TEVRLGLGAV RHIGDDLAEK LVQERKANGP FTSLLDLTAR 910 920 930 940 950 960 LQLSVQQTEA LATAGAFGCF GMSRREALWA AGAAATQRPD RLPGVGSSSH IPALPGMSEL 970 980 990 1000 1010 1020 ELAAADVWAT GISPDSYPTQ FLRDDLDAMG VVPAARLGSV PDGDRVLIAG AVTHRQRPGT 1030 1040 1050 1060 1070 1080 AQGVTFLNLE DETGMVNVLC TPGVWARHRK LANTAPALLV RGQVQNASGA ITVVAERLGR 1090 ITLAVGSRSR DFR

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE016958 Genomic DNA. Translation: AAS06026.1.
RefSeq	NP_962410.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2721197.
GenomeReviews	Gene locus MAP_3476c in contig AE016958_GR.
KEGG	mpa:MAP3476c.
NMPDR	fig|262316.1.peg.3476.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q73U92.
OMA	Q73U92. MGFYSPD.
Enzyme and pathway databases
BRENDA	2.7.7.7. 189774.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_MYCPA

Accession

Primary (citable) accession number: Q73U92

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	July 5, 2004
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)