Q73TT7 (ILVD_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	MAP_3631c

Organism

Mycobacterium paratuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1770 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium avium complex (MAC)

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 575	575	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000225397
Sites
	Metal binding	137	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	214	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q73TT7 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 0F590CF6C13364FF	FASTA	575	59,561
	10 20 30 40 50 60 MPTTDSARAA DIKQPDIKPR SRDVTDGLEK AAARGMLRAV GMGDEDFAKP QIGVASSWNE 70 80 90 100 110 120 ITPCNLSLDR LAKAVKEGVF AAGGYPLEFG TISVSDGISM GHEGMHFSLV SREVIADSVE 130 140 150 160 170 180 TVMQAERLDG SVLLAGCDKS LPGMLMAAAR LDLASVFLYA GSILPGVAKL SDGSEREVTI 190 200 210 220 230 240 IDAFEAVGAC ARGLMPREDV DAIERAICPG EGACGGMYTA NTMASAAEAL GMSLPGSAAP 250 260 270 280 290 300 PATDRRRDGF ARRSGQAVVE LLRRGITARD ILTKEAFENA IAVVMAFGGS TNAVLHLLAI 310 320 330 340 350 360 AHEADVALSL DDFSRIGSKV PHLADVKPFG RHVMTDVDHI GGVPVMMKAL LDAGLLNGDC 370 380 390 400 410 420 LTVTGATVAQ NLAAIAPPDP DGKVLRALSD PLHPTGGITI LRGSLAPEGA VVKSAGFDSD 430 440 450 460 470 480 VFEGTARVFD GERAALDALE DGTITKGDAV VIRYEGPKGG PGMREMLAIT GAIKGAGLGK 490 500 510 520 530 540 DVLLLTDGRF SGGTTGLCVG HIAPEAVDAG PIAFLRDGDR IRLDVANRVL DVLVDPAEFD 550 560 570 SRRTGFTPPP PRYKTGVLAK YVKLVGSAAI GAVCG

References

[1]

"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis." Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V. Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed: 16116077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE016958 Genomic DNA. Translation: AAS06181.1.
RefSeq	NP_962565.1. NC_002944.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000037928; EBMYCP00000036317; EBMYCG00000037923.
GeneID	2720233.
GenomeReviews	Gene locus MAP_3631c in contig AE016958_GR.
KEGG	mpa:MAP3631c.
NMPDR	fig|262316.1.peg.3631.
PATRIC	18000109. VBIMycAvi108102_3861.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000016366.
HOGENOM	HBG671001.
OMA	NMPGAMI.
ProtClustDB	PRK00911.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_MYCPA

Accession

Primary (citable) accession number: Q73TT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program