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Q73TS5 (TRMB_MYCPA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:MAP_3643c
OrganismMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10) [Complete proteome] [HAMAP]
Taxonomic identifier262316 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)

Protein attributes

Sequence length259 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 259259tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_0000171357

Regions

Region238 – 2414Substrate binding By similarity

Sites

Active site1661 By similarity
Binding site911S-adenosyl-L-methionine By similarity
Binding site1161S-adenosyl-L-methionine By similarity
Binding site1431S-adenosyl-L-methionine By similarity
Binding site1661S-adenosyl-L-methionine By similarity
Binding site1701Substrate By similarity
Binding site2021Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q73TS5 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 5DDA5DFA345BE461

FASTA25928,411
        10         20         30         40         50         60 
MGHHGQMHAQ HGVTMPADSP IGQPDRSEQR YFPATAFRTR RSTLSDAQRQ TWDRRWPELG 

        70         80         90        100        110        120 
LSVGPAEDPD RPGPPLDTDA WFGRKAPVVL EIGCGNGTST LAMAKEEPGV DVIAVEVYRR 

       130        140        150        160        170        180 
GLAQLLCAID RDNVTNIRLI RGNALDVLQR LIAPASLTGV RVFFPDPWPK ARHHKRRFLQ 

       190        200        210        220        230        240 
PGTVGLIADR LLPGGVLHVA TDHAGYAEHI ADVGAGEPRL RPANPDSPLP ISVARPTTKY 

       250 
ETKAQDAGSA VTEFIWLRR 

« Hide

References

[1]"The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-968 / K-10.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE016958 Genomic DNA. Translation: AAS06193.1.
RefSeqNP_962577.1. NC_002944.2.

3D structure databases

ProteinModelPortalQ73TS5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING262316.MAP3643c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS06193; AAS06193; MAP_3643c.
GeneID2719251.
KEGGmpa:MAP3643c.
PATRIC18000131. VBIMycAvi108102_3872.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
KOK03439.
OMATEILQDC.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycMAVI262316:GCQR-3687-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_MYCPA
AccessionPrimary (citable) accession number: Q73TS5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways