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Protein

Acetate kinase

Gene

ackA

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction.UniRule annotation

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. Phosphate acetyltransferase (pta)
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi14MagnesiumUniRule annotation1
Binding sitei21ATPUniRule annotation1
Binding sitei80SubstrateUniRule annotation1
Active sitei137Proton donor/acceptorUniRule annotation1
Sitei169Transition state stabilizerUniRule annotation1
Sitei230Transition state stabilizerUniRule annotation1
Metal bindingi373MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi197 – 201ATPUniRule annotation5
Nucleotide bindingi271 – 273ATPUniRule annotation3
Nucleotide bindingi319 – 323ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMAVI262316:G1G0E-4074-MONOMER.
UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Ordered Locus Names:MAP_3886
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi
  • UP000000580 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004219471 – 387Acetate kinaseAdd BLAST387

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi262316.MAP3886.

Structurei

Secondary structure

1387
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 15Combined sources7
Beta strandi20 – 26Combined sources7
Turni27 – 30Combined sources4
Beta strandi31 – 38Combined sources8
Helixi51 – 64Combined sources14
Turni69 – 73Combined sources5
Beta strandi74 – 82Combined sources9
Turni85 – 87Combined sources3
Helixi96 – 103Combined sources8
Helixi104 – 108Combined sources5
Turni110 – 112Combined sources3
Helixi113 – 126Combined sources14
Beta strandi130 – 136Combined sources7
Helixi139 – 141Combined sources3
Helixi146 – 149Combined sources4
Helixi155 – 160Combined sources6
Helixi170 – 183Combined sources14
Helixi188 – 190Combined sources3
Beta strandi192 – 208Combined sources17
Beta strandi211 – 216Combined sources6
Beta strandi223 – 225Combined sources3
Helixi237 – 245Combined sources9
Helixi250 – 259Combined sources10
Helixi262 – 267Combined sources6
Helixi273 – 280Combined sources8
Helixi284 – 308Combined sources25
Beta strandi313 – 317Combined sources5
Helixi318 – 323Combined sources6
Helixi325 – 332Combined sources8
Helixi336 – 338Combined sources3
Helixi344 – 347Combined sources4
Beta strandi353 – 356Combined sources4
Beta strandi363 – 368Combined sources6
Helixi373 – 384Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3R9PX-ray1.90A/B1-387[»]
ProteinModelPortaliQ73T33.
SMRiQ73T33.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ73T33.

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
KOiK00925.
OMAiWKALTGT.
OrthoDBiPOG091H02K4.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiView protein in InterPro
IPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiView protein in Pfam
PF00871. Acetate_kinase. 1 hit.
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiView protein in PROSITE
PS01075. ACETATE_KINASE_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q73T33-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGSDGARRV LVINSGSSSL KFQLVDPEFG VAASTGIVER IGEESSPVPD
60 70 80 90 100
HDAALRRAFD MLAGDGVDLN TAGLVAVGHR VVHGGNTFYR PTVLDDAVIA
110 120 130 140 150
RLHELSELAP LHNPPALQGI EVARRLLPDI AHVAVFDTGF FHDLPPAAAT
160 170 180 190 200
YAIDRELADR WQIRRYGFHG TSHRYVSEQA AAFLDRPLRG LKQIVLHLGN
210 220 230 240 250
GCSASAIAGT RPLDTSMGLT PLEGLVMGTR SGDIDPSIVS YLCHTAGMGV
260 270 280 290 300
DDVESMLNHR SGVVGLSGVR DFRRLRELIE SGDGAAQLAY SVFTHRLRKY
310 320 330 340 350
IGAYLAVLGH TDVISFTAGI GENDAAVRRD AVSGMEELGI VLDERRNLAG
360 370 380
GKGARQISAD DSPITVLVVP TNEELAIARD CVRVLGG
Length:387
Mass (Da):41,310
Last modified:July 5, 2004 - v1
Checksum:i3608EB4D41710C0F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS06436.1.
RefSeqiWP_003873824.1. NC_002944.2.

Genome annotation databases

EnsemblBacteriaiAAS06436; AAS06436; MAP_3886.
GeneIDi2721509.
KEGGimpa:MAP_3886.
PATRICifig|262316.17.peg.4137.

Similar proteinsi

Entry informationi

Entry nameiACKA_MYCPA
AccessioniPrimary (citable) accession number: Q73T33
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2013
Last sequence update: July 5, 2004
Last modified: February 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome