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Protein

Putative glutamate--cysteine ligase 2

Gene

MAP_3922

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

ATP-dependent carboxylate-amine ligase which exhibits weak glutamate--cysteine ligase activity.UniRule annotation

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMAVI262316:GCQR-3967-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative glutamate--cysteine ligase 2UniRule annotation (EC:6.3.2.2UniRule annotation)
Alternative name(s):
Gamma-glutamylcysteine synthetase 2UniRule annotation
Short name:
GCS 2UniRule annotation
Short name:
Gamma-GCS 2UniRule annotation
Gene namesi
Ordered Locus Names:MAP_3922
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi
  • UP000000580 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 376376Putative glutamate--cysteine ligase 2PRO_0000218207Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi262316.MAP3922.

Structurei

3D structure databases

ProteinModelPortaliQ73T00.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glutamate--cysteine ligase type 2 family. YbdK subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106KXD. Bacteria.
COG2170. LUCA.
KOiK06048.
OMAiLIFGLHV.
OrthoDBiEOG6P336Z.

Family and domain databases

HAMAPiMF_01609. Glu_cys_ligase_2.
InterProiIPR006336. GCS2.
IPR011793. YbdK.
[Graphical view]
PfamiPF04107. GCS2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02050. gshA_cyan_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

Q73T00-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPARRGDAHI DFARSPRPTI GVEWEFALVD AQTRDLSNEA TAVIAEIGEN
60 70 80 90 100
PRVHKELLRN TVEVVSGICR TVPEAMEDLR QTLGPARRIV RDRGMELFCA
110 120 130 140 150
GAHPFAQWTT QKLTDAPRYA ELIKRTQWWG RQMLIWGVHV HVGISSPNKV
160 170 180 190 200
MPIMTSLLNY YPHLLALSAS SPWWTGVDTG YASNRAMMFQ QLPTAGLPFQ
210 220 230 240 250
FQTWAEFEGF VYDQKKTGII DHVDEVRWDI RPSPHLGTLE MRICDGVSNL
260 270 280 290 300
HELAALVALT HCLVVDLDRR LEADESLPTM PPWHHQENKW RAARYGLDAV
310 320 330 340 350
IILDADSNER LVTEDLDDVL NRLEPVARKL QCADELAAVA DIPRHGASYQ
360 370
RQRRVAEEHD GDLRAVVDAL VAELEI
Length:376
Mass (Da):42,552
Last modified:April 26, 2005 - v2
Checksum:iC7089754BB14E87F
GO

Sequence cautioni

The sequence AAS06472.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS06472.1. Different initiation.
RefSeqiWP_015632730.1. NC_002944.2.

Genome annotation databases

EnsemblBacteriaiAAS06472; AAS06472; MAP_3922.
GeneIDi2719338.
KEGGimpa:MAP_3922.
PATRICi18000736. VBIMycAvi108102_4174.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS06472.1. Different initiation.
RefSeqiWP_015632730.1. NC_002944.2.

3D structure databases

ProteinModelPortaliQ73T00.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262316.MAP3922.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS06472; AAS06472; MAP_3922.
GeneIDi2719338.
KEGGimpa:MAP_3922.
PATRICi18000736. VBIMycAvi108102_4174.

Phylogenomic databases

eggNOGiENOG4106KXD. Bacteria.
COG2170. LUCA.
KOiK06048.
OMAiLIFGLHV.
OrthoDBiEOG6P336Z.

Enzyme and pathway databases

BioCyciMAVI262316:GCQR-3967-MONOMER.

Family and domain databases

HAMAPiMF_01609. Glu_cys_ligase_2.
InterProiIPR006336. GCS2.
IPR011793. YbdK.
[Graphical view]
PfamiPF04107. GCS2. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02050. gshA_cyan_rel. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
    Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
    Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-968 / K-10.

Entry informationi

Entry nameiGCS2_MYCPA
AccessioniPrimary (citable) accession number: Q73T00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: March 16, 2016
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.