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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 3811D-inositol 3-phosphateUniRule annotation
Binding sitei52 – 521UDP-GlcNAc; via amide nitrogenUniRule annotation
Binding sitei107 – 10711D-inositol 3-phosphateUniRule annotation
Binding sitei140 – 14011D-inositol 3-phosphateUniRule annotation
Binding sitei164 – 16411D-inositol 3-phosphateUniRule annotation
Binding sitei184 – 18411D-inositol 3-phosphateUniRule annotation
Binding sitei258 – 2581UDP-GlcNAcUniRule annotation
Binding sitei263 – 2631UDP-GlcNAcUniRule annotation
Binding sitei316 – 3161UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation
Metal bindingi325 – 3251Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi326 – 3261Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi328 – 3281Magnesium; via carbonyl oxygenUniRule annotation
Binding sitei338 – 3381UDP-GlcNAcUniRule annotation
Binding sitei346 – 3461UDP-GlcNAcUniRule annotation
Metal bindingi352 – 3521MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMAVI262316:GCQR-4024-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:MAP_3979
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi
  • UP000000580 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 441441D-inositol 3-phosphate glycosyltransferasePRO_0000400137Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi262316.MAP3979.

Structurei

3D structure databases

ProteinModelPortaliQ73SU4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni44 – 452UDP-GlcNAc bindingUniRule annotation
Regioni49 – 5461D-inositol 3-phosphate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiEOG647TVR.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q73SU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCCALGPGGE PSCKDDRVRH DDVFRLNEPR RVAVLAVHTS PLAQPGTGDA
60 70 80 90 100
GGMNVYVLQT ALHLARRGIE VEIFTRATAS ADPPVQRVAP GVLVRNVVAG
110 120 130 140 150
PFEGLDKYDL PTQLCAFAAG VLRAEASHEP GYYDIVHSHY WLSGQVGWLA
160 170 180 190 200
RDRWAVPLVH TAHTLAAVKN AALAQGDSPE PPLRTVGEQQ VVDEADRLIV
210 220 230 240 250
NTDDEARQLI SIHHADPARI DVVHPGVDLD VFRPGDRRAA RAALGLPLDK
260 270 280 290 300
DIVAFVGRIQ PLKAPDIVLR AAAKLPGVRI VVAGGPSGTG LASPDGLARL
310 320 330 340 350
ADELGISARV TFLPPQSRPN LATLFQAANL VAVPSYSESF GLVALEAQAC
360 370 380 390 400
GTPVAAAAVG GLPVAVRDGV TGTLVAGHDV DHWADALAGL LRAPAPAAAA
410 420 430 440
MSRAAAAHAA TFSWDHTTDA LLASYRRAIR EYTTERRGVG A
Length:441
Mass (Da):46,334
Last modified:July 5, 2004 - v1
Checksum:i122EB4E502E4921D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS06529.1.

Genome annotation databases

EnsemblBacteriaiAAS06529; AAS06529; MAP_3979.
KEGGimpa:MAP_3979.
PATRICi18000852. VBIMycAvi108102_4232.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS06529.1.

3D structure databases

ProteinModelPortaliQ73SU4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262316.MAP3979.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS06529; AAS06529; MAP_3979.
KEGGimpa:MAP_3979.
PATRICi18000852. VBIMycAvi108102_4232.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiEOG647TVR.

Enzyme and pathway databases

BioCyciMAVI262316:GCQR-4024-MONOMER.

Family and domain databases

HAMAPiMF_01695. MshA.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The complete genome sequence of Mycobacterium avium subspecies paratuberculosis."
    Li L., Bannantine J.P., Zhang Q., Amonsin A., May B.J., Alt D., Banerji N., Kanjilal S., Kapur V.
    Proc. Natl. Acad. Sci. U.S.A. 102:12344-12349(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-968 / K-10.

Entry informationi

Entry nameiMSHA_MYCPA
AccessioniPrimary (citable) accession number: Q73SU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: July 5, 2004
Last modified: May 11, 2016
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.