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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei381D-inositol 3-phosphateUniRule annotation1
Binding sitei52UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei1071D-inositol 3-phosphateUniRule annotation1
Binding sitei1401D-inositol 3-phosphateUniRule annotation1
Binding sitei1641D-inositol 3-phosphateUniRule annotation1
Binding sitei1841D-inositol 3-phosphateUniRule annotation1
Binding sitei258UDP-GlcNAcUniRule annotation1
Binding sitei263UDP-GlcNAcUniRule annotation1
Binding sitei316UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi325Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi326Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi328Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei338UDP-GlcNAcUniRule annotation1
Binding sitei346UDP-GlcNAcUniRule annotation1
Metal bindingi352MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:MAP_3979
OrganismiMycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
Taxonomic identifieri262316 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium avium complex (MAC)
Proteomesi
  • UP000000580 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004001371 – 441D-inositol 3-phosphate glycosyltransferaseAdd BLAST441

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi262316.MAP3979.

Structurei

3D structure databases

ProteinModelPortaliQ73SU4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni44 – 45UDP-GlcNAc bindingUniRule annotation2
Regioni49 – 541D-inositol 3-phosphate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q73SU4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCCALGPGGE PSCKDDRVRH DDVFRLNEPR RVAVLAVHTS PLAQPGTGDA
60 70 80 90 100
GGMNVYVLQT ALHLARRGIE VEIFTRATAS ADPPVQRVAP GVLVRNVVAG
110 120 130 140 150
PFEGLDKYDL PTQLCAFAAG VLRAEASHEP GYYDIVHSHY WLSGQVGWLA
160 170 180 190 200
RDRWAVPLVH TAHTLAAVKN AALAQGDSPE PPLRTVGEQQ VVDEADRLIV
210 220 230 240 250
NTDDEARQLI SIHHADPARI DVVHPGVDLD VFRPGDRRAA RAALGLPLDK
260 270 280 290 300
DIVAFVGRIQ PLKAPDIVLR AAAKLPGVRI VVAGGPSGTG LASPDGLARL
310 320 330 340 350
ADELGISARV TFLPPQSRPN LATLFQAANL VAVPSYSESF GLVALEAQAC
360 370 380 390 400
GTPVAAAAVG GLPVAVRDGV TGTLVAGHDV DHWADALAGL LRAPAPAAAA
410 420 430 440
MSRAAAAHAA TFSWDHTTDA LLASYRRAIR EYTTERRGVG A
Length:441
Mass (Da):46,334
Last modified:July 5, 2004 - v1
Checksum:i122EB4E502E4921D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS06529.1.

Genome annotation databases

EnsemblBacteriaiAAS06529; AAS06529; MAP_3979.
KEGGimpa:MAP_3979.
PATRICi18000852. VBIMycAvi108102_4232.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE016958 Genomic DNA. Translation: AAS06529.1.

3D structure databases

ProteinModelPortaliQ73SU4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi262316.MAP3979.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS06529; AAS06529; MAP_3979.
KEGGimpa:MAP_3979.
PATRICi18000852. VBIMycAvi108102_4232.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_MYCPA
AccessioniPrimary (citable) accession number: Q73SU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: July 5, 2004
Last modified: November 2, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.