Q73N18 (ISPE_TREDE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	TDE_1338

Organism

Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222) [Complete proteome] [HAMAP]

Taxonomic identifier

243275 [NCBI]

Taxonomic lineage

Bacteria › Spirochaetes › Spirochaetales › Spirochaetaceae › Treponema

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000189281
Regions
	Nucleotide binding	96 – 106	11	ATP Potential
Sites
	Active site	13	1	By similarity
	Active site	138	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q73N18 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 24B74277A7ED8C07	FASTA	284	31,226
	10 20 30 40 50 60 MIKSAISLKA HAKINLHLEV LGKRSDGFHD IVSVFAPISL ADELLMQRKP DKKECKVLSP 70 80 90 100 110 120 LAELPAENTI TRAYEEFKNF AGISEGISVR ILKRIPEGAG LGGGSSDAAS VLRGLNDMFS 130 140 150 160 170 180 TGLAEEDLRA IALKIGSDVP FFLGNEAAVV RGRGETIKRV SVFSDYFGIL IYPEIKSATP 190 200 210 220 230 240 RAYSLLGRKE SEILNPAFNP ELFCGKDCRE WPFFNSFEDV LFTEYPAIKK AKQDLLTNGA 250 260 270 280 DFALMSGAGS SVFGLFKNEK TVKNAYSKLL AEYGRCFFFL LLAF

References

[1]

"Comparison of the genome of the oral pathogen Treponema denticola with other spirochete genomes." Seshadri R., Myers G.S.A., Tettelin H., Eisen J.A., Heidelberg J.F., Dodson R.J., Davidsen T.M., DeBoy R.T., Fouts D.E., Haft D.H., Selengut J., Ren Q., Brinkac L.M., Madupu R., Kolonay J.F., Durkin S.A., Daugherty S.C., Shetty J. , Shvartsbeyn A., Gebregeorgis E., Geer K., Tsegaye G., Malek J.A., Ayodeji B., Shatsman S., McLeod M.P., Smajs D., Howell J.K., Pal S., Amin A., Vashisth P., McNeill T.Z., Xiang Q., Sodergren E., Baca E., Weinstock G.M., Norris S.J., Fraser C.M., Paulsen I.T. Proc. Natl. Acad. Sci. U.S.A. 101:5646-5651(2004) [PubMed: 15064399] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35405 / CIP 103919 / DSM 14222.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017226 Genomic DNA. Translation: AAS11855.1.
RefSeq	NP_971944.1. NC_002967.9.
3D structure databases
ProteinModelPortal	Q73N18.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2741072.
GenomeReviews	Gene locus TDE_1338 in contig AE017226_GR.
KEGG	tde:TDE1338.
NMPDR	fig|243275.1.peg.1327.
PATRIC	20524866. VBITreDen445_1286.
TIGR	TDE_1338.
Phylogenomic databases
HOGENOM	HBG734593.
OMA	VSTAEVY.
ProtClustDB	CLSK218665.
Enzyme and pathway databases
BioCyc	TDEN243275:TDE_1338-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_TREDE

Accession

Primary (citable) accession number: Q73N18

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program