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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei629ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTDEN243275:G1G0Q-2712-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:TDE_2663
OrganismiTreponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222)
Taxonomic identifieri243275 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
Proteomesi
  • UP000008212 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985681 – 1100Isoleucine--tRNA ligaseAdd BLAST1100

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243275.TDE2663

Structurei

3D structure databases

ProteinModelPortaliQ73JB2
SMRiQ73JB2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi48 – 58"HIGH" regionAdd BLAST11
Motifi626 – 630"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit

Sequencei

Sequence statusi: Complete.

Q73JB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKPVDPKVD FAKQEEDVLK FWEKNDVFKK SVSSRDGRDN YIFFDGPPFA
60 70 80 90 100
TGLPHFGHFV PGTIKDIIPR YKTMKGFRVE RRFGWDCHGL PVENLIEKEL
110 120 130 140 150
GLNSKTDIEK YGIDKFNEAC RASVLRYVKE WKQTITRLGR WVDFENDYKT
160 170 180 190 200
MEPAFMESIW WVMKSLWEKG LLYEGYYILP YCPRCSTVLS NHELNLGGYK
210 220 230 240 250
DVHDPAITVR FKTLSPVKTS PAGKAFEGKN ALPSDTYLLA WTTTPWTLPS
260 270 280 290 300
NLGLAVGADI DYALIEYDGA HYIMAVPRLE AYFAKSGKEE AKEYKLIWTK
310 320 330 340 350
KGAELEGLRY EPLFPYFKNL AADENGKNAE AGQGAFRVLI GDFVTTEDGT
360 370 380 390 400
GIVHTAPGFG EDDNRIFKDT GVPTVCPVDA ECKFTHEVSD YQGLFVKDAD
410 420 430 440 450
KQIMERLKTE GKLFKKAQIL HSYPHCWRCS SPLIYRAVAS WFVSVTKIKD
460 470 480 490 500
KLLNANSKIN WQPDHIKTGR FGKWLEGARD WAISRNRYWG NPIPIWKCPD
510 520 530 540 550
CGETICVGSR EELKELSGVF PEDMHKHFVD KISIPCKKCG GTMKRVSEVL
560 570 580 590 600
DCWFESGSMP YAQQHYPFEN KEHFEKNFPA DFISEGLDQT RGWFYTLTIL
610 620 630 640 650
AAALFDEPAF KNCIVNGLVL AEDGKKMSKS LRNYTDPNEV IKQFGADALR
660 670 680 690 700
LFLMNSNVVK ADDLKYSDEG VRDVLKGILI PFWNSYSFYI TYANIDGVKP
710 720 730 740 750
PHNAKVDGKD EGVEEFLAKL NNPLDLWILS VTEKLVADVT EALDKYDLSQ
760 770 780 790 800
AIPPMVEYID LLNNWYIRRS RRRFWKSEND GDKSQAYETL YRALKKFSLV
810 820 830 840 850
AAPVVPFITE SIWQNLRTES DALSIHLADY PDYNEKIRNS ELEFKMKTVQ
860 870 880 890 900
KAVSMGRALR YQFNLKIRQP LKAVEIVTLN PEEKRVLLEM EESIIEELNV
910 920 930 940 950
KEVIFHEKED ELVEYSAKAN FKVLGKELGP LMKKAAAIIE QMNSSEIQNI
960 970 980 990 1000
MEGATLSIDI EGKSVEITAD KIVINRIEKA SLKIVNEGTL TVGLNTELTE
1010 1020 1030 1040 1050
ELLMEGYIRD LVRGIQTLRK ECGLDVTDRI KLYLSASQKN ADNKELEKAF
1060 1070 1080 1090 1100
ELFKDYVCDE TLTVQSSWLK TGELTKLGSI KTSLVEAGDY EWEIGIEKNN
Length:1,100
Mass (Da):125,760
Last modified:July 5, 2004 - v1
Checksum:i90A17F4C6F320E8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017226 Genomic DNA Translation: AAS13180.1
RefSeqiNP_973261.1, NC_002967.9
WP_002680710.1, NC_002967.9

Genome annotation databases

EnsemblBacteriaiAAS13180; AAS13180; TDE_2663
GeneIDi2741108
KEGGitde:TDE2663
PATRICifig|243275.7.peg.2517

Similar proteinsi

Entry informationi

Entry nameiSYI_TREDE
AccessioniPrimary (citable) accession number: Q73JB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: March 28, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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