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Q73JB2

- SYI_TREDE

UniProt

Q73JB2 - SYI_TREDE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei629 – 6291ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTDEN243275:GJ7G-2712-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:TDE_2663
OrganismiTreponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222)
Taxonomic identifieri243275 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
ProteomesiUP000008212: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11001100Isoleucine--tRNA ligasePRO_0000098568Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243275.TDE2663.

Structurei

3D structure databases

ProteinModelPortaliQ73JB2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionAdd
BLAST
Motifi626 – 6305"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q73JB2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYKPVDPKVD FAKQEEDVLK FWEKNDVFKK SVSSRDGRDN YIFFDGPPFA
60 70 80 90 100
TGLPHFGHFV PGTIKDIIPR YKTMKGFRVE RRFGWDCHGL PVENLIEKEL
110 120 130 140 150
GLNSKTDIEK YGIDKFNEAC RASVLRYVKE WKQTITRLGR WVDFENDYKT
160 170 180 190 200
MEPAFMESIW WVMKSLWEKG LLYEGYYILP YCPRCSTVLS NHELNLGGYK
210 220 230 240 250
DVHDPAITVR FKTLSPVKTS PAGKAFEGKN ALPSDTYLLA WTTTPWTLPS
260 270 280 290 300
NLGLAVGADI DYALIEYDGA HYIMAVPRLE AYFAKSGKEE AKEYKLIWTK
310 320 330 340 350
KGAELEGLRY EPLFPYFKNL AADENGKNAE AGQGAFRVLI GDFVTTEDGT
360 370 380 390 400
GIVHTAPGFG EDDNRIFKDT GVPTVCPVDA ECKFTHEVSD YQGLFVKDAD
410 420 430 440 450
KQIMERLKTE GKLFKKAQIL HSYPHCWRCS SPLIYRAVAS WFVSVTKIKD
460 470 480 490 500
KLLNANSKIN WQPDHIKTGR FGKWLEGARD WAISRNRYWG NPIPIWKCPD
510 520 530 540 550
CGETICVGSR EELKELSGVF PEDMHKHFVD KISIPCKKCG GTMKRVSEVL
560 570 580 590 600
DCWFESGSMP YAQQHYPFEN KEHFEKNFPA DFISEGLDQT RGWFYTLTIL
610 620 630 640 650
AAALFDEPAF KNCIVNGLVL AEDGKKMSKS LRNYTDPNEV IKQFGADALR
660 670 680 690 700
LFLMNSNVVK ADDLKYSDEG VRDVLKGILI PFWNSYSFYI TYANIDGVKP
710 720 730 740 750
PHNAKVDGKD EGVEEFLAKL NNPLDLWILS VTEKLVADVT EALDKYDLSQ
760 770 780 790 800
AIPPMVEYID LLNNWYIRRS RRRFWKSEND GDKSQAYETL YRALKKFSLV
810 820 830 840 850
AAPVVPFITE SIWQNLRTES DALSIHLADY PDYNEKIRNS ELEFKMKTVQ
860 870 880 890 900
KAVSMGRALR YQFNLKIRQP LKAVEIVTLN PEEKRVLLEM EESIIEELNV
910 920 930 940 950
KEVIFHEKED ELVEYSAKAN FKVLGKELGP LMKKAAAIIE QMNSSEIQNI
960 970 980 990 1000
MEGATLSIDI EGKSVEITAD KIVINRIEKA SLKIVNEGTL TVGLNTELTE
1010 1020 1030 1040 1050
ELLMEGYIRD LVRGIQTLRK ECGLDVTDRI KLYLSASQKN ADNKELEKAF
1060 1070 1080 1090 1100
ELFKDYVCDE TLTVQSSWLK TGELTKLGSI KTSLVEAGDY EWEIGIEKNN
Length:1,100
Mass (Da):125,760
Last modified:July 5, 2004 - v1
Checksum:i90A17F4C6F320E8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017226 Genomic DNA. Translation: AAS13180.1.
RefSeqiNP_973261.1. NC_002967.9.

Genome annotation databases

EnsemblBacteriaiAAS13180; AAS13180; TDE_2663.
GeneIDi2741108.
KEGGitde:TDE2663.
PATRICi20527384. VBITreDen445_2517.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017226 Genomic DNA. Translation: AAS13180.1 .
RefSeqi NP_973261.1. NC_002967.9.

3D structure databases

ProteinModelPortali Q73JB2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243275.TDE2663.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS13180 ; AAS13180 ; TDE_2663 .
GeneIDi 2741108.
KEGGi tde:TDE2663.
PATRICi 20527384. VBITreDen445_2517.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TDEN243275:GJ7G-2712-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35405 / CIP 103919 / DSM 14222.

Entry informationi

Entry nameiSYI_TREDE
AccessioniPrimary (citable) accession number: Q73JB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3