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Q73JB2

- SYI_TREDE

UniProt

Q73JB2 - SYI_TREDE

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Protein
Isoleucine--tRNA ligase
Gene
ileS, TDE_2663
Organism
Treponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei629 – 6291ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciTDEN243275:GJ7G-2712-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:TDE_2663
OrganismiTreponema denticola (strain ATCC 35405 / CIP 103919 / DSM 14222)
Taxonomic identifieri243275 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesSpirochaetaceaeTreponema
ProteomesiUP000008212: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11001100Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098568Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi243275.TDE2663.

Structurei

3D structure databases

ProteinModelPortaliQ73JB2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi48 – 5811"HIGH" regionUniRule annotation
Add
BLAST
Motifi626 – 6305"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
KOiK01870.
OMAiKWIISEI.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q73JB2-1 [UniParc]FASTAAdd to Basket

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MYKPVDPKVD FAKQEEDVLK FWEKNDVFKK SVSSRDGRDN YIFFDGPPFA     50
TGLPHFGHFV PGTIKDIIPR YKTMKGFRVE RRFGWDCHGL PVENLIEKEL 100
GLNSKTDIEK YGIDKFNEAC RASVLRYVKE WKQTITRLGR WVDFENDYKT 150
MEPAFMESIW WVMKSLWEKG LLYEGYYILP YCPRCSTVLS NHELNLGGYK 200
DVHDPAITVR FKTLSPVKTS PAGKAFEGKN ALPSDTYLLA WTTTPWTLPS 250
NLGLAVGADI DYALIEYDGA HYIMAVPRLE AYFAKSGKEE AKEYKLIWTK 300
KGAELEGLRY EPLFPYFKNL AADENGKNAE AGQGAFRVLI GDFVTTEDGT 350
GIVHTAPGFG EDDNRIFKDT GVPTVCPVDA ECKFTHEVSD YQGLFVKDAD 400
KQIMERLKTE GKLFKKAQIL HSYPHCWRCS SPLIYRAVAS WFVSVTKIKD 450
KLLNANSKIN WQPDHIKTGR FGKWLEGARD WAISRNRYWG NPIPIWKCPD 500
CGETICVGSR EELKELSGVF PEDMHKHFVD KISIPCKKCG GTMKRVSEVL 550
DCWFESGSMP YAQQHYPFEN KEHFEKNFPA DFISEGLDQT RGWFYTLTIL 600
AAALFDEPAF KNCIVNGLVL AEDGKKMSKS LRNYTDPNEV IKQFGADALR 650
LFLMNSNVVK ADDLKYSDEG VRDVLKGILI PFWNSYSFYI TYANIDGVKP 700
PHNAKVDGKD EGVEEFLAKL NNPLDLWILS VTEKLVADVT EALDKYDLSQ 750
AIPPMVEYID LLNNWYIRRS RRRFWKSEND GDKSQAYETL YRALKKFSLV 800
AAPVVPFITE SIWQNLRTES DALSIHLADY PDYNEKIRNS ELEFKMKTVQ 850
KAVSMGRALR YQFNLKIRQP LKAVEIVTLN PEEKRVLLEM EESIIEELNV 900
KEVIFHEKED ELVEYSAKAN FKVLGKELGP LMKKAAAIIE QMNSSEIQNI 950
MEGATLSIDI EGKSVEITAD KIVINRIEKA SLKIVNEGTL TVGLNTELTE 1000
ELLMEGYIRD LVRGIQTLRK ECGLDVTDRI KLYLSASQKN ADNKELEKAF 1050
ELFKDYVCDE TLTVQSSWLK TGELTKLGSI KTSLVEAGDY EWEIGIEKNN 1100
Length:1,100
Mass (Da):125,760
Last modified:July 5, 2004 - v1
Checksum:i90A17F4C6F320E8D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017226 Genomic DNA. Translation: AAS13180.1.
RefSeqiNP_973261.1. NC_002967.9.

Genome annotation databases

EnsemblBacteriaiAAS13180; AAS13180; TDE_2663.
GeneIDi2741108.
KEGGitde:TDE2663.
PATRICi20527384. VBITreDen445_2517.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017226 Genomic DNA. Translation: AAS13180.1 .
RefSeqi NP_973261.1. NC_002967.9.

3D structure databases

ProteinModelPortali Q73JB2.
ModBasei Search...

Protein-protein interaction databases

STRINGi 243275.TDE2663.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS13180 ; AAS13180 ; TDE_2663 .
GeneIDi 2741108.
KEGGi tde:TDE2663.
PATRICi 20527384. VBITreDen445_2517.

Phylogenomic databases

eggNOGi COG0060.
KOi K01870.
OMAi KWIISEI.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci TDEN243275:GJ7G-2712-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 35405 / CIP 103919 / DSM 14222.

Entry informationi

Entry nameiSYI_TREDE
AccessioniPrimary (citable) accession number: Q73JB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi