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Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Wolbachia pipientis wMel
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi261 – 2611Manganese 2UniRule annotation
Metal bindingi266 – 2661Manganese 1UniRule annotation
Metal bindingi266 – 2661Manganese 2UniRule annotation
Active sitei273 – 2731UniRule annotation
Metal bindingi284 – 2841Manganese 2UniRule annotation
Metal bindingi343 – 3431Manganese 1UniRule annotation
Metal bindingi345 – 3451Manganese 1UniRule annotation
Metal bindingi345 – 3451Manganese 2UniRule annotation
Active sitei347 – 3471UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciWEND163164:GJ8W-55-MONOMER.

Protein family/group databases

MEROPSiM17.950.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
Alternative name(s):
Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
Short name:
LAPUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:WD_0054
OrganismiWolbachia pipientis wMel
Taxonomic identifieri163164 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia
ProteomesiUP000008215 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Probable cytosol aminopeptidasePRO_0000165814Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi163164.WD0054.

Structurei

3D structure databases

ProteinModelPortaliQ73IU2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243129.
KOiK01255.
OMAiEINRSWG.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q73IU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSLQLFATE IPAMKITISK ISPDFKTIVM GLFEDNETVN DGGVLQGKQV
60 70 80 90 100
IDNIKQFSDF NGSFGEFFST ALPEEKNVIV VGLGKKDEWN ENKELNIGGK
110 120 130 140 150
IYCELSRLKI KKAAVLIEGS AANVAYGAFL RSFKFDKYKT KKDEKITEVE
160 170 180 190 200
EITVLVKDEQ LSNAERSFEH LRQEGESIFL ARSFITEPPN ILYPESYADH
210 220 230 240 250
IKKELTKLGL EIEVLDKKQM EEKKMGALLG VAQGSSKEPK LVVIKWNGAS
260 270 280 290 300
KEQKPIAFVG KGITFDTGGV SLKPSRGMES MKYDMAGSAT VVGVMHALAG
310 320 330 340 350
RKAKVNAIGV VALAENAVGG NAQRPSDVVT SMSGQTIEVL NTDAEGRLIL
360 370 380 390 400
ADALWYTQDR FSPKFMIDLA TLTGAIVVAL GNNEYAGLFS NNDELANRLI
410 420 430 440 450
DAGNEVNEKL WRFPMNETYD KIIDSPIADV QNIAPAGSGG DSIMAAQFLQ
460 470 480 490 500
RFVNETCWAH LDIAGTAWHE KGTDICPRGA VGFGVRLLNK LVEKYYEAND
Length:500
Mass (Da):54,922
Last modified:July 5, 2004 - v1
Checksum:iC82ECF4D51EFEFB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017196 Genomic DNA. Translation: AAS13818.1.

Genome annotation databases

EnsemblBacteriaiAAS13818; AAS13818; WD_0054.
KEGGiwol:WD0054.
PATRICi24028675. VBIWolEnd21207_0053.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017196 Genomic DNA. Translation: AAS13818.1.

3D structure databases

ProteinModelPortaliQ73IU2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi163164.WD0054.

Protein family/group databases

MEROPSiM17.950.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS13818; AAS13818; WD_0054.
KEGGiwol:WD0054.
PATRICi24028675. VBIWolEnd21207_0053.

Phylogenomic databases

eggNOGiCOG0260.
HOGENOMiHOG000243129.
KOiK01255.
OMAiEINRSWG.
OrthoDBiEOG6FV8B3.

Enzyme and pathway databases

BioCyciWEND163164:GJ8W-55-MONOMER.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiAMPA_WOLPM
AccessioniPrimary (citable) accession number: Q73IU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: July 5, 2004
Last modified: July 22, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.