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Q73IU2 (AMPA_WOLPM) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable cytosol aminopeptidase

EC=3.4.11.1
Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase
Gene names
Name:pepA
Ordered Locus Names:WD_0054
OrganismWolbachia pipientis wMel [Complete proteome] [HAMAP]
Taxonomic identifier163164 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP-Rule MF_00181

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP-Rule MF_00181

Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactor

Binds 2 manganese ions per subunit By similarity. HAMAP-Rule MF_00181

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00181.

Sequence similarities

Belongs to the peptidase M17 family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandManganese
Metal-binding
   Molecular functionAminopeptidase
Hydrolase
Protease
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionaminopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metalloexopeptidase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Probable cytosol aminopeptidase HAMAP-Rule MF_00181
PRO_0000165814

Sites

Active site2731 Potential
Active site3471 Potential
Metal binding2611Manganese 2 By similarity
Metal binding2661Manganese 1 By similarity
Metal binding2661Manganese 2 By similarity
Metal binding2841Manganese 2 By similarity
Metal binding3431Manganese 1 By similarity
Metal binding3451Manganese 1 By similarity
Metal binding3451Manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q73IU2 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: C82ECF4D51EFEFB5

FASTA50054,922
        10         20         30         40         50         60 
MYSLQLFATE IPAMKITISK ISPDFKTIVM GLFEDNETVN DGGVLQGKQV IDNIKQFSDF 

        70         80         90        100        110        120 
NGSFGEFFST ALPEEKNVIV VGLGKKDEWN ENKELNIGGK IYCELSRLKI KKAAVLIEGS 

       130        140        150        160        170        180 
AANVAYGAFL RSFKFDKYKT KKDEKITEVE EITVLVKDEQ LSNAERSFEH LRQEGESIFL 

       190        200        210        220        230        240 
ARSFITEPPN ILYPESYADH IKKELTKLGL EIEVLDKKQM EEKKMGALLG VAQGSSKEPK 

       250        260        270        280        290        300 
LVVIKWNGAS KEQKPIAFVG KGITFDTGGV SLKPSRGMES MKYDMAGSAT VVGVMHALAG 

       310        320        330        340        350        360 
RKAKVNAIGV VALAENAVGG NAQRPSDVVT SMSGQTIEVL NTDAEGRLIL ADALWYTQDR 

       370        380        390        400        410        420 
FSPKFMIDLA TLTGAIVVAL GNNEYAGLFS NNDELANRLI DAGNEVNEKL WRFPMNETYD 

       430        440        450        460        470        480 
KIIDSPIADV QNIAPAGSGG DSIMAAQFLQ RFVNETCWAH LDIAGTAWHE KGTDICPRGA 

       490        500 
VGFGVRLLNK LVEKYYEAND 

« Hide

References

[1]"Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements."
Wu M., Sun L.V., Vamathevan J.J., Riegler M., DeBoy R.T., Brownlie J.C., McGraw E.A., Martin W., Esser C., Ahmadinejad N., Wiegand C., Madupu R., Beanan M.J., Brinkac L.M., Daugherty S.C., Durkin A.S., Kolonay J.F., Nelson W.C. expand/collapse author list , Mohamoud Y., Lee P., Berry K.J., Young M.B., Utterback T.R., Weidman J.F., Nierman W.C., Paulsen I.T., Nelson K.E., Tettelin H., O'Neill S.L., Eisen J.A.
PLoS Biol. 2:327-341(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017196 Genomic DNA. Translation: AAS13818.1.
RefSeqNP_965884.1. NC_002978.6.

3D structure databases

ProteinModelPortalQ73IU2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING163164.WD0054.

Protein family/group databases

MEROPSM17.003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS13818; AAS13818; WD_0054.
GeneID2738311.
KEGGwol:WD0054.
PATRIC24028675. VBIWolEnd21207_0053.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0260.
HOGENOMHOG000243129.
KOK01255.
OMADSQFADM.
OrthoDBEOG6FV8B3.

Enzyme and pathway databases

BioCycWEND163164:GJ8W-55-MONOMER.

Family and domain databases

HAMAPMF_00181. Cytosol_peptidase_M17.
InterProIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSPR00481. LAMNOPPTDASE.
PROSITEPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPA_WOLPM
AccessionPrimary (citable) accession number: Q73IU2
Entry history
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: July 5, 2004
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries