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Q73IT7 (SYL_WOLPM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:WD_0060
OrganismWolbachia pipientis wMel [Complete proteome] [HAMAP]
Taxonomic identifier163164 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia

Protein attributes

Sequence length838 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 838838Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152118

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00049
Motif611 – 6155"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6141ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q73IT7 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 81D296221BF255A8

FASTA83895,661
        10         20         30         40         50         60 
MKYDFKSVEK FYQDKWDFSV SKSGKQEKCY VLEMFPYPSG KIHMGHLRNY AIGDVIARYK 

        70         80         90        100        110        120 
RANGFEVLHP IGWDAFGLPA ENAARDNNIS PETWTKENID NMRTQLKSIG LSYNWERELS 

       130        140        150        160        170        180 
TCEPDYYKHE QKFFLDFLKH GLAYRKESWV NWDPVDQTVL ANEQVVDGKG WRSGAVVEKR 

       190        200        210        220        230        240 
KLSQWFLKIT DFAEDLLKCL QSLKNWPEKV KTMQERWIGK SEGATIEFEV VGLNKKLKVF 

       250        260        270        280        290        300 
TTYPHTLFGA SFCAVAAEHP IVQDLKNGSS VVIPVLDTGI QEIKSKREND EKIGVYTGLN 

       310        320        330        340        350        360 
VKHPFLDKEL PLYIANFVLM EYGEGAIFGC PAHDQRDFEF AQKYNLPIIP VISSAHLGVI 

       370        380        390        400        410        420 
PARDQNSYNG SQCQATQMTK EAYTGDGTMF NSEFLNGLMV SEAKEAIVKK FEEKKIGKKT 

       430        440        450        460        470        480 
INYRLHDWGV SRQRYWGCPI PIIYCKDCGT VPVPEKDLPV VLPIDVEFTS GGNPLDKHPT 

       490        500        510        520        530        540 
WKFVDCPKCG KQAERETDTF DTFFESSWYF AAFCSEDKSI DKDACNRFMP VDYYIGGIEH 

       550        560        570        580        590        600 
AILHLLYSRF FCRALTKCGY FDIKEPFSTL ITQGMVCHAT YKDENGKWLF PAEAKELIAR 

       610        620        630        640        650        660 
GAKVQVGKVE KMSKSKKNTV DPNFIIEKYG ADTARLFVLS DTPPEKDMEW SDDGVEGCSR 

       670        680        690        700        710        720 
YVNKLWRMVM QLRPVNIHYD NENVTGGLLE YRKKIHKLLH GLTDDLENCR LNCVVAKFRE 

       730        740        750        760        770        780 
MTNLIAEIDV KTGKSLIDEG ICILIRVIEP FIPHLAESLW QEIGGQPWPK ADESLLVDDT 

       790        800        810        820        830 
VTIAVQINGK LRTTIKVAIN LPQEELKKIA IDSVSSKIDQ SKVRTVYAVP NKIVNIVI 

« Hide

References

[1]"Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements."
Wu M., Sun L.V., Vamathevan J.J., Riegler M., DeBoy R.T., Brownlie J.C., McGraw E.A., Martin W., Esser C., Ahmadinejad N., Wiegand C., Madupu R., Beanan M.J., Brinkac L.M., Daugherty S.C., Durkin A.S., Kolonay J.F., Nelson W.C. expand/collapse author list , Mohamoud Y., Lee P., Berry K.J., Young M.B., Utterback T.R., Weidman J.F., Nierman W.C., Paulsen I.T., Nelson K.E., Tettelin H., O'Neill S.L., Eisen J.A.
PLoS Biol. 2:327-341(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017196 Genomic DNA. Translation: AAS13823.1.
RefSeqNP_965889.1. NC_002978.6.

3D structure databases

ProteinModelPortalQ73IT7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING163164.WD0060.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS13823; AAS13823; WD_0060.
GeneID2738664.
KEGGwol:WD0060.
PATRIC24028685. VBIWolEnd21207_0058.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycWEND163164:GJ8W-60-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_WOLPM
AccessionPrimary (citable) accession number: Q73IT7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries