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Q73IQ8 (DDL_WOLPM) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:WD_0095
OrganismWolbachia pipientis wMel [Complete proteome] [HAMAP]
Taxonomic identifier163164 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341195

Regions

Domain101 – 297197ATP-grasp
Nucleotide binding128 – 18154ATP By similarity

Sites

Metal binding2491Magnesium or manganese 1 By similarity
Metal binding2631Magnesium or manganese 1 By similarity
Metal binding2631Magnesium or manganese 2 By similarity
Metal binding2651Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q73IQ8 [UniParc].

Last modified July 5, 2004. Version 1.
Checksum: 87868BA2A031C118

FASTA31535,482
        10         20         30         40         50         60 
MIPTIAILSG GFSCEREISL MSGKAVKKAL DSLSYNAIEI DVDSNIAEKL KKINPGLAFI 

        70         80         90        100        110        120 
ALHGPYGEDG CIQGLLEILG IKYTHSGVMA SAVAINKVMS KHIFRSLNID TPKGYVISRE 

       130        140        150        160        170        180 
DVLKNNIKID YPYVLKPINE GSSIGVYIIF SHEDYLELKN NSSTIMEKMI VEEYIPGIEL 

       190        200        210        220        230        240 
HTAVLLDEAI GTIEVRPKNK FYDYEAKYTD GFAEHIFPAK IPDNIYKMTL EHALKIHQFL 

       250        260        270        280        290        300 
GCKTISRSDF RYNPKNNTLK MLEINTHPGF TELSLVPEIA KLAKGINFNE LVKIIIEDSL 

       310 
QHKNIRDLSH VEQYY 

« Hide

References

[1]"Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements."
Wu M., Sun L.V., Vamathevan J.J., Riegler M., DeBoy R.T., Brownlie J.C., McGraw E.A., Martin W., Esser C., Ahmadinejad N., Wiegand C., Madupu R., Beanan M.J., Brinkac L.M., Daugherty S.C., Durkin A.S., Kolonay J.F., Nelson W.C. expand/collapse author list , Mohamoud Y., Lee P., Berry K.J., Young M.B., Utterback T.R., Weidman J.F., Nierman W.C., Paulsen I.T., Nelson K.E., Tettelin H., O'Neill S.L., Eisen J.A.
PLoS Biol. 2:327-341(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE017196 Genomic DNA. Translation: AAS13853.1.
RefSeqNP_965919.1. NC_002978.6.

3D structure databases

ProteinModelPortalQ73IQ8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING163164.WD0095.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAS13853; AAS13853; WD_0095.
GeneID2738006.
KEGGwol:WD0095.
PATRIC24028761. VBIWolEnd21207_0095.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAEDPARES.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycWEND163164:GJ8W-94-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_WOLPM
AccessionPrimary (citable) accession number: Q73IQ8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: July 5, 2004
Last modified: May 14, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways