Q73I23 (ISPE_WOLPM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	WD_0360

Organism

Wolbachia pipientis wMel [Complete proteome] [HAMAP]

Taxonomic identifier

163164 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000189290
Regions
	Nucleotide binding	100 – 110	11	ATP Potential
Sites
	Active site	11	1	By similarity
	Active site	140	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q73I23 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: B4F6A25679DD4134	FASTA	288	31,878
	10 20 30 40 50 60 MKSFCVKAPA KINLFLHVVD KKETGYHLIE GLFVFANLSN FLEIKVGEKD SRYDNSTVEF 70 80 90 100 110 120 INSESKINNQ YNTVMKAVNL LLRHAPVRTK VTVKVVKNIP IAAGLGSGSS DAGAVVRTLG 130 140 150 160 170 180 KLWEIDRTIL NEIALNVGAD VPASVDSKPV FVRGIGEELC HIKKFSLPTN VVLVKPKKRF 190 200 210 220 230 240 LSTPEVFSKH EGKFSEPIKW SDDAEKDLLK FLKETRNDLQ EIAISFVPEI KDVISTLKSQ 250 260 270 280 EGSILSRMSG SGVSCFGIFD SEENAKAAAV NIGKKQPEWW VCNTQLIV

References

[1]

"Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements." Wu M., Sun L.V., Vamathevan J.J., Riegler M., DeBoy R.T., Brownlie J.C., McGraw E.A., Martin W., Esser C., Ahmadinejad N., Wiegand C., Madupu R., Beanan M.J., Brinkac L.M., Daugherty S.C., Durkin A.S., Kolonay J.F., Nelson W.C. , Mohamoud Y., Lee P., Berry K.J., Young M.B., Utterback T.R., Weidman J.F., Nierman W.C., Paulsen I.T., Nelson K.E., Tettelin H., O'Neill S.L., Eisen J.A. PLoS Biol. 2:327-341(2004) [PubMed: 15024419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017196 Genomic DNA. Translation: AAS14089.1.
RefSeq	NP_966155.1. NC_002978.6.
3D structure databases
ProteinModelPortal	Q73I23.
ModBase	Search...
Protein-protein interaction databases
STRING	Q73I23.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBWOLT00000004503; EBWOLP00000004060; EBWOLG00000004503.
GeneID	2738159.
GenomeReviews	Gene locus WD_0360 in contig AE017196_GR.
KEGG	wol:WD0360.
PATRIC	24029306. VBIWolEnd21207_0362.
TIGR	WD_0360.
Phylogenomic databases
GeneTree	EBGT00050000030880.
HOGENOM	HBG734593.
OMA	CQLARMS.
PhylomeDB	Q73I23.
ProtClustDB	PRK14610.
Enzyme and pathway databases
BioCyc	WPIP955:WD_0360-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_WOLPM

Accession

Primary (citable) accession number: Q73I23

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program