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Q73HW7

- SYI_WOLPM

UniProt

Q73HW7 - SYI_WOLPM

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Wolbachia pipientis wMel
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (05 Jul 2004)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei648 – 6481ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciWEND163164:GJ8W-417-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:WD_0423
    OrganismiWolbachia pipientis wMel
    Taxonomic identifieri163164 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia
    ProteomesiUP000008215: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11111111Isoleucine--tRNA ligasePRO_0000098572Add
    BLAST

    Proteomic databases

    PRIDEiQ73HW7.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi163164.WD0423.

    Structurei

    3D structure databases

    ProteinModelPortaliQ73HW7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi52 – 6211"HIGH" regionAdd
    BLAST
    Motifi645 – 6495"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiENRDWFE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 3 hits.
    SSF50677. SSF50677. 2 hits.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q73HW7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSKHYPDTV SSPDFLSLEK EIIKFWQENK VFERSVEERS KDNCFVFYDG     50
    PPFANGLPHY GHLLTGFIKD AFARYQTMLQ KRVERRFGWD CHGLPAEMGA 100
    EKELGISGRT EIEKFGIDKF NDHCRTSVMK FSSEWEKYVN RQARWVDFHN 150
    DYKTMDKSFM ESVMWAFKQL YDKGLVYESV RVVPYSWACE TPLSNFETRL 200
    DNAYREKVSK AVTVAFELLE NPQQFKSVKR KCKLLAWTTT PWTLPSNLAL 250
    AIGKDIKYCA VSVHPLMSFQ RVTLESISGS QCLGTGMTGS SEGSSMNGEI 300
    YIFAESYLEK FISHSEQNNI PYENCNIKLK ANDLAGLSYK PLFDYFKDTK 350
    NAFRVFIADY VTEEDGTGVV HTAPGFGEED FYLCQSHDIP VICPIDNSGK 400
    FTAEVSDLAG VHVFDANDTV IKKLKGQGSW FKTEQYIHNY PHCWRTDTPL 450
    IYRTMPSWYV AVTKFKSRMV ELNKRVNWIP NHIRDGQFGK WLEGAHDWSI 500
    SRNRFWGTPI PVWKSDDARY PRVDVYGSIE ELERDFNVKI DDLHRPFIDT 550
    LTRLNPDDPT GKSVMRRVPD VFDCWFESGS MPFAQIHYPF ENKEWFESAD 600
    FITEYIAQTR GWFYTLFVLS TALFNREPFK NCICHGVVLD VKGQKLSKRL 650
    NNYADPMEVF DRYGSDALRF LMLSGSIICG GNLLLDKEGN SIRDVLKNVI 700
    KPIWNSYHFF TMYANADGIK AEVCKDYQST IDRYMISKCF EAVESIQASM 750
    NSYSSQEACK ILIDFFEVLN NWYIRRSRER FWKSDLDQDK TDAYNVLYTV 800
    FYYILRAAAP LLPLITENIW QGLKYEETSV HLANFPQLEK FDSQLIAKMD 850
    LVREVCNSAL SIRNTFNIRI RQPLGSMIIY HQSSCSFLEG EPLSVIPEFF 900
    PVIQVADTGI QCAADSNEYQ EMIKDEVNVK SLELVNRLEG IASLELKLNF 950
    PLLGKRIPDK IKKLVQYVKE GKWKQVENEQ IFLGDESENY IIEKGEYELL 1000
    LKANSEYSSV FDNNKGIVIL NTELNDELIL EGLARDVVRL IQETRKQADF 1050
    HISDRIRVII KTEEEKIKEA INTWFEYIKE QTLALSLDIN TEIGTNFYSK 1100
    EYQDLSVSIE R 1111
    Length:1,111
    Mass (Da):128,833
    Last modified:July 5, 2004 - v1
    Checksum:iA8673A51D27841F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017196 Genomic DNA. Translation: AAS14146.1.
    RefSeqiNP_966212.1. NC_002978.6.
    WP_010962579.1. NC_002978.6.

    Genome annotation databases

    EnsemblBacteriaiAAS14146; AAS14146; WD_0423.
    GeneIDi2738789.
    KEGGiwol:WD0423.
    PATRICi24029422. VBIWolEnd21207_0419.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE017196 Genomic DNA. Translation: AAS14146.1 .
    RefSeqi NP_966212.1. NC_002978.6.
    WP_010962579.1. NC_002978.6.

    3D structure databases

    ProteinModelPortali Q73HW7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 163164.WD0423.

    Proteomic databases

    PRIDEi Q73HW7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAS14146 ; AAS14146 ; WD_0423 .
    GeneIDi 2738789.
    KEGGi wol:WD0423.
    PATRICi 24029422. VBIWolEnd21207_0419.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi ENRDWFE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci WEND163164:GJ8W-417-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 2 hits.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 3 hits.
    SSF50677. SSF50677. 2 hits.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiSYI_WOLPM
    AccessioniPrimary (citable) accession number: Q73HW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: July 5, 2004
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3