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Q73HW7

- SYI_WOLPM

UniProt

Q73HW7 - SYI_WOLPM

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Protein

Isoleucine--tRNA ligase

Gene
ileS, WD_0423
Organism
Wolbachia pipientis wMel
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei648 – 6481ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciWEND163164:GJ8W-417-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:WD_0423
OrganismiWolbachia pipientis wMel
Taxonomic identifieri163164 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia
ProteomesiUP000008215: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11111111Isoleucine--tRNA ligaseUniRule annotationPRO_0000098572Add
BLAST

Proteomic databases

PRIDEiQ73HW7.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi163164.WD0423.

Structurei

3D structure databases

ProteinModelPortaliQ73HW7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi52 – 6211"HIGH" regionUniRule annotationAdd
BLAST
Motifi645 – 6495"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 3 hits.
SSF50677. SSF50677. 2 hits.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q73HW7-1 [UniParc]FASTAAdd to Basket

« Hide

MKSKHYPDTV SSPDFLSLEK EIIKFWQENK VFERSVEERS KDNCFVFYDG     50
PPFANGLPHY GHLLTGFIKD AFARYQTMLQ KRVERRFGWD CHGLPAEMGA 100
EKELGISGRT EIEKFGIDKF NDHCRTSVMK FSSEWEKYVN RQARWVDFHN 150
DYKTMDKSFM ESVMWAFKQL YDKGLVYESV RVVPYSWACE TPLSNFETRL 200
DNAYREKVSK AVTVAFELLE NPQQFKSVKR KCKLLAWTTT PWTLPSNLAL 250
AIGKDIKYCA VSVHPLMSFQ RVTLESISGS QCLGTGMTGS SEGSSMNGEI 300
YIFAESYLEK FISHSEQNNI PYENCNIKLK ANDLAGLSYK PLFDYFKDTK 350
NAFRVFIADY VTEEDGTGVV HTAPGFGEED FYLCQSHDIP VICPIDNSGK 400
FTAEVSDLAG VHVFDANDTV IKKLKGQGSW FKTEQYIHNY PHCWRTDTPL 450
IYRTMPSWYV AVTKFKSRMV ELNKRVNWIP NHIRDGQFGK WLEGAHDWSI 500
SRNRFWGTPI PVWKSDDARY PRVDVYGSIE ELERDFNVKI DDLHRPFIDT 550
LTRLNPDDPT GKSVMRRVPD VFDCWFESGS MPFAQIHYPF ENKEWFESAD 600
FITEYIAQTR GWFYTLFVLS TALFNREPFK NCICHGVVLD VKGQKLSKRL 650
NNYADPMEVF DRYGSDALRF LMLSGSIICG GNLLLDKEGN SIRDVLKNVI 700
KPIWNSYHFF TMYANADGIK AEVCKDYQST IDRYMISKCF EAVESIQASM 750
NSYSSQEACK ILIDFFEVLN NWYIRRSRER FWKSDLDQDK TDAYNVLYTV 800
FYYILRAAAP LLPLITENIW QGLKYEETSV HLANFPQLEK FDSQLIAKMD 850
LVREVCNSAL SIRNTFNIRI RQPLGSMIIY HQSSCSFLEG EPLSVIPEFF 900
PVIQVADTGI QCAADSNEYQ EMIKDEVNVK SLELVNRLEG IASLELKLNF 950
PLLGKRIPDK IKKLVQYVKE GKWKQVENEQ IFLGDESENY IIEKGEYELL 1000
LKANSEYSSV FDNNKGIVIL NTELNDELIL EGLARDVVRL IQETRKQADF 1050
HISDRIRVII KTEEEKIKEA INTWFEYIKE QTLALSLDIN TEIGTNFYSK 1100
EYQDLSVSIE R 1111
Length:1,111
Mass (Da):128,833
Last modified:July 5, 2004 - v1
Checksum:iA8673A51D27841F7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017196 Genomic DNA. Translation: AAS14146.1.
RefSeqiNP_966212.1. NC_002978.6.
WP_010962579.1. NC_002978.6.

Genome annotation databases

EnsemblBacteriaiAAS14146; AAS14146; WD_0423.
GeneIDi2738789.
KEGGiwol:WD0423.
PATRICi24029422. VBIWolEnd21207_0419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017196 Genomic DNA. Translation: AAS14146.1 .
RefSeqi NP_966212.1. NC_002978.6.
WP_010962579.1. NC_002978.6.

3D structure databases

ProteinModelPortali Q73HW7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 163164.WD0423.

Proteomic databases

PRIDEi Q73HW7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS14146 ; AAS14146 ; WD_0423 .
GeneIDi 2738789.
KEGGi wol:WD0423.
PATRICi 24029422. VBIWolEnd21207_0419.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci WEND163164:GJ8W-417-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 3 hits.
SSF50677. SSF50677. 2 hits.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSYI_WOLPM
AccessioniPrimary (citable) accession number: Q73HW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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