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Q73HW7

- SYI_WOLPM

UniProt

Q73HW7 - SYI_WOLPM

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Wolbachia pipientis wMel
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei648 – 6481ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciWEND163164:GJ8W-417-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:WD_0423
OrganismiWolbachia pipientis wMel
Taxonomic identifieri163164 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia
ProteomesiUP000008215: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11111111Isoleucine--tRNA ligasePRO_0000098572Add
BLAST

Proteomic databases

PRIDEiQ73HW7.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi163164.WD0423.

Structurei

3D structure databases

ProteinModelPortaliQ73HW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi52 – 6211"HIGH" regionAdd
BLAST
Motifi645 – 6495"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiENRDWFE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 3 hits.
SSF50677. SSF50677. 2 hits.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q73HW7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSKHYPDTV SSPDFLSLEK EIIKFWQENK VFERSVEERS KDNCFVFYDG
60 70 80 90 100
PPFANGLPHY GHLLTGFIKD AFARYQTMLQ KRVERRFGWD CHGLPAEMGA
110 120 130 140 150
EKELGISGRT EIEKFGIDKF NDHCRTSVMK FSSEWEKYVN RQARWVDFHN
160 170 180 190 200
DYKTMDKSFM ESVMWAFKQL YDKGLVYESV RVVPYSWACE TPLSNFETRL
210 220 230 240 250
DNAYREKVSK AVTVAFELLE NPQQFKSVKR KCKLLAWTTT PWTLPSNLAL
260 270 280 290 300
AIGKDIKYCA VSVHPLMSFQ RVTLESISGS QCLGTGMTGS SEGSSMNGEI
310 320 330 340 350
YIFAESYLEK FISHSEQNNI PYENCNIKLK ANDLAGLSYK PLFDYFKDTK
360 370 380 390 400
NAFRVFIADY VTEEDGTGVV HTAPGFGEED FYLCQSHDIP VICPIDNSGK
410 420 430 440 450
FTAEVSDLAG VHVFDANDTV IKKLKGQGSW FKTEQYIHNY PHCWRTDTPL
460 470 480 490 500
IYRTMPSWYV AVTKFKSRMV ELNKRVNWIP NHIRDGQFGK WLEGAHDWSI
510 520 530 540 550
SRNRFWGTPI PVWKSDDARY PRVDVYGSIE ELERDFNVKI DDLHRPFIDT
560 570 580 590 600
LTRLNPDDPT GKSVMRRVPD VFDCWFESGS MPFAQIHYPF ENKEWFESAD
610 620 630 640 650
FITEYIAQTR GWFYTLFVLS TALFNREPFK NCICHGVVLD VKGQKLSKRL
660 670 680 690 700
NNYADPMEVF DRYGSDALRF LMLSGSIICG GNLLLDKEGN SIRDVLKNVI
710 720 730 740 750
KPIWNSYHFF TMYANADGIK AEVCKDYQST IDRYMISKCF EAVESIQASM
760 770 780 790 800
NSYSSQEACK ILIDFFEVLN NWYIRRSRER FWKSDLDQDK TDAYNVLYTV
810 820 830 840 850
FYYILRAAAP LLPLITENIW QGLKYEETSV HLANFPQLEK FDSQLIAKMD
860 870 880 890 900
LVREVCNSAL SIRNTFNIRI RQPLGSMIIY HQSSCSFLEG EPLSVIPEFF
910 920 930 940 950
PVIQVADTGI QCAADSNEYQ EMIKDEVNVK SLELVNRLEG IASLELKLNF
960 970 980 990 1000
PLLGKRIPDK IKKLVQYVKE GKWKQVENEQ IFLGDESENY IIEKGEYELL
1010 1020 1030 1040 1050
LKANSEYSSV FDNNKGIVIL NTELNDELIL EGLARDVVRL IQETRKQADF
1060 1070 1080 1090 1100
HISDRIRVII KTEEEKIKEA INTWFEYIKE QTLALSLDIN TEIGTNFYSK
1110
EYQDLSVSIE R
Length:1,111
Mass (Da):128,833
Last modified:July 5, 2004 - v1
Checksum:iA8673A51D27841F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017196 Genomic DNA. Translation: AAS14146.1.
RefSeqiNP_966212.1. NC_002978.6.
WP_010962579.1. NC_002978.6.

Genome annotation databases

EnsemblBacteriaiAAS14146; AAS14146; WD_0423.
GeneIDi2738789.
KEGGiwol:WD0423.
PATRICi24029422. VBIWolEnd21207_0419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017196 Genomic DNA. Translation: AAS14146.1 .
RefSeqi NP_966212.1. NC_002978.6.
WP_010962579.1. NC_002978.6.

3D structure databases

ProteinModelPortali Q73HW7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 163164.WD0423.

Proteomic databases

PRIDEi Q73HW7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS14146 ; AAS14146 ; WD_0423 .
GeneIDi 2738789.
KEGGi wol:WD0423.
PATRICi 24029422. VBIWolEnd21207_0419.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ENRDWFE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci WEND163164:GJ8W-417-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 2 hits.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 3 hits.
SSF50677. SSF50677. 2 hits.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiSYI_WOLPM
AccessioniPrimary (citable) accession number: Q73HW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: July 5, 2004
Last modified: November 26, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3