Q73HV5 (SYE1_WOLPM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 1
Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	WD_0435

Organism

Wolbachia pipientis wMel [Complete proteome] [HAMAP]

Taxonomic identifier

163164 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Glutamate--tRNA ligase 1 HAMAP MF_00022_B		PRO_0000119697
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00022_B
	Motif	252 – 256	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	255	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q73HV5 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: AE03505EC7327E51	FASTA	473	53,968
	10 20 30 40 50 60 MPGIVTRFAP SPTGFLHIGG ARTALFNWLY AKHHGGRFLL RIEDTDRKRS TQEAIDAIIE 70 80 90 100 110 120 GLRWLGMSYD GEIVYQSKRI ERHKEVANLL VEKGRAYHCY CPEDEVAEKK AKAREEGKIY 130 140 150 160 170 180 KHKCTTKPPS CHPSSRHWKN MWSRAGMTSG VRSVVRFKVP DSQEIVIDDK IYGQIKVNSD 190 200 210 220 230 240 QLDDIVILRS DNTPTYIFAV VVDDHDAGIT DIIRGSDHLT NTFKHLLIYQ ALDFDIPRFA 250 260 270 280 290 300 HVPLIHGEDG NKLSKRHGAT SVCDYEKMGI LPKAMRNYLL RLGWSHGNDE IISDEQAIEW 310 320 330 340 350 360 FNLESIGRSP ARLDFKKLEH LNNHYISNMS NEDILTLMLR ENTLTDKKKG YLLQGLTELK 370 380 390 400 410 420 KRANYLTELL DLAKFYIQDP PLDLSEEAEQ IVKSNLDIIK LLASFLSKIG DENWNKGFLS 430 440 450 460 470 SQIKECAKLH DMKISDVYHS LRAPITGVMD APGIIDIMVI LGKDECIRRL QAI

References

[1]

"Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements." Wu M., Sun L.V., Vamathevan J.J., Riegler M., DeBoy R.T., Brownlie J.C., McGraw E.A., Martin W., Esser C., Ahmadinejad N., Wiegand C., Madupu R., Beanan M.J., Brinkac L.M., Daugherty S.C., Durkin A.S., Kolonay J.F., Nelson W.C. , Mohamoud Y., Lee P., Berry K.J., Young M.B., Utterback T.R., Weidman J.F., Nierman W.C., Paulsen I.T., Nelson K.E., Tettelin H., O'Neill S.L., Eisen J.A. PLoS Biol. 2:327-341(2004) [PubMed: 15024419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017196 Genomic DNA. Translation: AAS14158.1.
RefSeq	NP_966224.1. NC_002978.6.
3D structure databases
ProteinModelPortal	Q73HV5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q73HV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBWOLT00000004018; EBWOLP00000003575; EBWOLG00000004018.
GeneID	2738807.
GenomeReviews	Gene locus WD_0435 in contig AE017196_GR.
KEGG	wol:WD0435.
PATRIC	24029446. VBIWolEnd21207_0431.
TIGR	WD_0435.
Phylogenomic databases
GeneTree	EBGT00050000030976.
HOGENOM	HBG628189.
OMA	ANFNDES.
PhylomeDB	Q73HV5.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	WPIP955:WD_0435-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_WOLPM

Accession

Primary (citable) accession number: Q73HV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	July 5, 2004
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program