Q73G71 (TRMB_WOLPM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	WD_1091

Organism

Wolbachia pipientis wMel [Complete proteome] [HAMAP]

Taxonomic identifier

163164 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000171422
Sites
	Binding site	51	1	S-adenosyl-L-methionine By similarity
	Binding site	76	1	S-adenosyl-L-methionine By similarity
	Binding site	103	1	S-adenosyl-L-methionine By similarity
	Binding site	125	1	S-adenosyl-L-methionine By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	161	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q73G71 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 4F3AC487C588A2B0	FASTA	221	26,251
	10 20 30 40 50 60 MLFKNSKWIG SFSRRSRLKP DVDEILEKYS IQNSKESIEK IVNSQKRIWV EIGFGNGENM 70 80 90 100 110 120 LYQVLNEPDL LFIGCEPYLK GVSRLLTNIE IQNIKNILMW TEDARELIAN FPDNSVERFF 130 140 150 160 170 180 ILFPDPWPKR SHNKRRLINT EFLNLLAKKI LITGEIFIAT DHQDYAEWIA SHIKQCNSLI 190 200 210 220 YREDDFTSYT LTKYHRRALK DQRKVRFFKV SVINNLQTMD Q

References

[1]

"Phylogenomics of the reproductive parasite Wolbachia pipientis wMel: a streamlined genome overrun by mobile genetic elements." Wu M., Sun L.V., Vamathevan J.J., Riegler M., DeBoy R.T., Brownlie J.C., McGraw E.A., Martin W., Esser C., Ahmadinejad N., Wiegand C., Madupu R., Beanan M.J., Brinkac L.M., Daugherty S.C., Durkin A.S., Kolonay J.F., Nelson W.C. , Mohamoud Y., Lee P., Berry K.J., Young M.B., Utterback T.R., Weidman J.F., Nierman W.C., Paulsen I.T., Nelson K.E., Tettelin H., O'Neill S.L., Eisen J.A. PLoS Biol. 2:327-341(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE017196 Genomic DNA. Translation: AAS14746.1.
RefSeq	NP_966812.1. NC_002978.6.
3D structure databases
ProteinModelPortal	Q73G71.
ModBase	Search...
Protein-protein interaction databases
STRING	163164.WD1091.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAS14746; AAS14746; WD_1091.
GeneID	2737760.
KEGG	wol:WD1091.
PATRIC	24030782. VBIWolEnd21207_1083.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000063439.
KO	K03439.
OMA	AEWIASH.
ProtClustDB	CLSK2306985.
Enzyme and pathway databases
BioCyc	WEND163164:GJ8W-1077-MONOMER.
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_WOLPM

Accession

Primary (citable) accession number: Q73G71

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 5, 2004
Last modified:	May 29, 2013
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program