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Q73G71

- TRMB_WOLPM

UniProt

Q73G71 - TRMB_WOLPM

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene
trmB, WD_1091
Organism
Wolbachia pipientis wMel
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511S-adenosyl-L-methionine By similarity
Binding sitei76 – 761S-adenosyl-L-methionine By similarity
Binding sitei103 – 1031S-adenosyl-L-methionine By similarity
Active sitei125 – 1251 By similarity
Binding sitei125 – 1251S-adenosyl-L-methionine By similarity
Binding sitei129 – 1291Substrate By similarity
Binding sitei161 – 1611Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciWEND163164:GJ8W-1077-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:WD_1091
OrganismiWolbachia pipientis wMel
Taxonomic identifieri163164 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia
ProteomesiUP000008215: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 221221tRNA (guanine-N(7)-)-methyltransferaseUniRule annotationPRO_0000171422Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi163164.WD1091.

Structurei

3D structure databases

ProteinModelPortaliQ73G71.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000063439.
KOiK03439.
OMAiAEWIASH.
OrthoDBiEOG6K6VBC.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q73G71-1 [UniParc]FASTAAdd to Basket

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MLFKNSKWIG SFSRRSRLKP DVDEILEKYS IQNSKESIEK IVNSQKRIWV    50
EIGFGNGENM LYQVLNEPDL LFIGCEPYLK GVSRLLTNIE IQNIKNILMW 100
TEDARELIAN FPDNSVERFF ILFPDPWPKR SHNKRRLINT EFLNLLAKKI 150
LITGEIFIAT DHQDYAEWIA SHIKQCNSLI YREDDFTSYT LTKYHRRALK 200
DQRKVRFFKV SVINNLQTMD Q 221
Length:221
Mass (Da):26,251
Last modified:July 5, 2004 - v1
Checksum:i4F3AC487C588A2B0
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017196 Genomic DNA. Translation: AAS14746.1.
RefSeqiNP_966812.1. NC_002978.6.
WP_010963037.1. NC_002978.6.

Genome annotation databases

EnsemblBacteriaiAAS14746; AAS14746; WD_1091.
GeneIDi2737760.
KEGGiwol:WD1091.
PATRICi24030782. VBIWolEnd21207_1083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE017196 Genomic DNA. Translation: AAS14746.1 .
RefSeqi NP_966812.1. NC_002978.6.
WP_010963037.1. NC_002978.6.

3D structure databases

ProteinModelPortali Q73G71.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 163164.WD1091.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAS14746 ; AAS14746 ; WD_1091 .
GeneIDi 2737760.
KEGGi wol:WD1091.
PATRICi 24030782. VBIWolEnd21207_1083.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000063439.
KOi K03439.
OMAi AEWIASH.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci WEND163164:GJ8W-1077-MONOMER.

Family and domain databases

HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiTRMB_WOLPM
AccessioniPrimary (citable) accession number: Q73G71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: September 3, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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