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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Wolbachia pipientis wMel
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511S-adenosyl-L-methionineUniRule annotation
Binding sitei76 – 761S-adenosyl-L-methionineUniRule annotation
Binding sitei103 – 1031S-adenosyl-L-methionineUniRule annotation
Active sitei125 – 1251By similarity
Binding sitei125 – 1251S-adenosyl-L-methionineUniRule annotation
Binding sitei129 – 1291SubstrateUniRule annotation
Binding sitei161 – 1611SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciWEND163164:GJ8W-1077-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:WD_1091
OrganismiWolbachia pipientis wMel
Taxonomic identifieri163164 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia
ProteomesiUP000008215: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 221221tRNA (guanine-N(7)-)-methyltransferasePRO_0000171422Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi163164.WD1091.

Structurei

3D structure databases

ProteinModelPortaliQ73G71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000063439.
KOiK03439.
OMAiRLINTEF.
OrthoDBiEOG6K6VBC.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q73G71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFKNSKWIG SFSRRSRLKP DVDEILEKYS IQNSKESIEK IVNSQKRIWV
60 70 80 90 100
EIGFGNGENM LYQVLNEPDL LFIGCEPYLK GVSRLLTNIE IQNIKNILMW
110 120 130 140 150
TEDARELIAN FPDNSVERFF ILFPDPWPKR SHNKRRLINT EFLNLLAKKI
160 170 180 190 200
LITGEIFIAT DHQDYAEWIA SHIKQCNSLI YREDDFTSYT LTKYHRRALK
210 220
DQRKVRFFKV SVINNLQTMD Q
Length:221
Mass (Da):26,251
Last modified:July 5, 2004 - v1
Checksum:i4F3AC487C588A2B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017196 Genomic DNA. Translation: AAS14746.1.
RefSeqiNP_966812.1. NC_002978.6.
WP_010963037.1. NC_002978.6.

Genome annotation databases

EnsemblBacteriaiAAS14746; AAS14746; WD_1091.
GeneIDi2737760.
KEGGiwol:WD1091.
PATRICi24030782. VBIWolEnd21207_1083.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE017196 Genomic DNA. Translation: AAS14746.1.
RefSeqiNP_966812.1. NC_002978.6.
WP_010963037.1. NC_002978.6.

3D structure databases

ProteinModelPortaliQ73G71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi163164.WD1091.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS14746; AAS14746; WD_1091.
GeneIDi2737760.
KEGGiwol:WD1091.
PATRICi24030782. VBIWolEnd21207_1083.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000063439.
KOiK03439.
OMAiRLINTEF.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciWEND163164:GJ8W-1077-MONOMER.

Family and domain databases

HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiTRMB_WOLPM
AccessioniPrimary (citable) accession number: Q73G71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 5, 2004
Last modified: January 7, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.