Reviewed, UniProtKB/Swiss-Prot Q73E50 (SYP2_BACC1)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase 2
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase 2
      Short name=ProRS 2

Gene names

Name:	proS2
Ordered Locus Names:	BCE_0510

Organism

Bacillus cereus (strain ATCC 10987) [Complete proteome] [HAMAP]

Taxonomic identifier

222523 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	Prolyl-tRNA synthetase 2 HAMAP MF_01571		PRO_0000249117

Sequences

	Sequence		Length	Mass (Da)
	Q73E50-1 [UniParc]. Last modified July 5, 2004. Version 1. Checksum: 5E2CD5A71EB4B9BE	FASTA	476	54,749
	10 20 30 40 50 60 MAKEQVQAIT KMEEDFAQWY TDIVKKAELV DYSSVKGCMI LRPYGYALWE NMQKVMDEKL 70 80 90 100 110 120 KATGHENVYM PMFIPESLLQ KEKDHVEGFA PEVAWVTHGG DEKLAERLCI RPTSETLFCE 130 140 150 160 170 180 HFSKIVQSYN DLPKLYNQWC SVVRWEKTTR PFLRTTEFLW QEGHTIHETA EESQAETLNI 190 200 210 220 230 240 LNLYASFCED YLAIPVIKGQ KTEKEKFAGA KATYTIESLM HDGKALQTGT SHNFGTNFSE 250 260 270 280 290 300 AFDIKFLDRN GKWQYVHQTS WGVSARMIGG LIMVHSDNNG LVMPPKVAPV QVVIVPIAQH 310 320 330 340 350 360 KEGVLAKATE LQEHIQKVAR VKIDASNKTP GWKFNEYEMK GIPIRLEVGP KDIEKNQVVL 370 380 390 400 410 420 VRRDTKEKEF IAMEQLEERI PALLEEIHNS LFNKAKVFRD ENTYSVTKFE EMKKVADEKQ 430 440 450 460 470 GFIKAMWCGE LACEEKLKEE VGVSSRCMPF EQEHLANECI CCGKEAKQMV YWGKAY

References

[1]

"The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1." Rasko D.A., Ravel J., Oekstad O.A., Helgason E., Cer R.Z., Jiang L., Shores K.A., Fouts D.E., Tourasse N.J., Angiuoli S.V., Kolonay J.F., Nelson W.C., Kolstoe A.-B., Fraser C.M., Read T.D. Nucleic Acids Res. 32:977-988(2004) [PubMed: 14960714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE017194 Genomic DNA. Translation: AAS39445.1.
RefSeq	NP_976837.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q73E50.
Genome annotation databases
GeneID	2750613.
GenomeReviews	Gene locus BCE_0510 in contig AE017194_GR.
KEGG	bca:BCE_0510.
NMPDR	fig|222523.1.peg.509.
TIGR	BCE_0510.
Phylogenomic databases
HOGENOM	Q73E50.
OMA	CIEAMMQ.
Family and domain databases
HAMAP	MF_01571. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR004499. Pro-tRNA-synth_IIa_pro-type. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00408. proS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP2_BACC1

Accession

Primary (citable) accession number: Q73E50

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	July 5, 2004
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)